Evaluation of UVLED disinfection for biofouling control during distribution of wastewater effluent
A commercially available UVLED flow-through device, operating at 40 mJ/cm2, was examined for biofilm control on irrigation pipe material fed by wastewater effluent. Biofouling was monitored through total coliform counts, crystal violet (CV) staining, and ATP analyses. A UV fluence of 40 mJ/cm2 at 28...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
IWA Publishing
2024-03-01
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| Series: | Water Reuse |
| Subjects: | |
| Online Access: | http://wreuse.iwaponline.com/content/14/1/80 |
| _version_ | 1797200653404078080 |
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| author | Tara Randall Inbar Shlomo Emma Wells Breanna Real Ben Ma Yarrow Linden Jasmine Gamboa Eran Friedler Karl G. Linden |
| author_facet | Tara Randall Inbar Shlomo Emma Wells Breanna Real Ben Ma Yarrow Linden Jasmine Gamboa Eran Friedler Karl G. Linden |
| author_sort | Tara Randall |
| collection | DOAJ |
| description | A commercially available UVLED flow-through device, operating at 40 mJ/cm2, was examined for biofilm control on irrigation pipe material fed by wastewater effluent. Biofouling was monitored through total coliform counts, crystal violet (CV) staining, and ATP analyses. A UV fluence of 40 mJ/cm2 at 280 nm retarded biofilm formation; however, complete biofilm prevention by UV treatment was not achieved despite a high inactivation of planktonic cells. After 5 days of the study, the total coliform and CV biofilm quantification assays between the UV-treated and control bioreactor coupons were not statistically different. The total coliform counts indicated a stable biofilm cell concentration was reached; the CV assay showed biofilm biomass accumulation with time. The ATP results revealed higher coupon ATP on the UV-treated coupons than the control coupons by day 5. The results provoke an interesting discussion surrounding the contribution of viable cells, represented by total coliforms, and extracellular polymeric substance (EPS) to total biofilm biomass. This study also highlighted a need for further investigation into the relationship between ATP responses and complex UV-stress responses of diverse microbial communities as opposed to pure bacteria cultures.
HIGHLIGHTS
A UV fluence of 40 mJ/cm2 at 280 nm slows biofilm formation on irrigation pipe material.;
Bioreactor effluent total coliform concentrations and crystal violet coupon accumulation were not statistically different between UV-treated and control bioreactors after being fed wastewater effluent for five days.;
ATP results revealed higher ATP on UV-treated coupons than control coupons after 5 days in bioreactors fed by wastewater effluent.; |
| first_indexed | 2024-04-24T07:35:04Z |
| format | Article |
| id | doaj.art-57062dc6c3d7409196331d6e792031f7 |
| institution | Directory Open Access Journal |
| issn | 2709-6092 2709-6106 |
| language | English |
| last_indexed | 2024-04-24T07:35:04Z |
| publishDate | 2024-03-01 |
| publisher | IWA Publishing |
| record_format | Article |
| series | Water Reuse |
| spelling | doaj.art-57062dc6c3d7409196331d6e792031f72024-04-20T06:52:33ZengIWA PublishingWater Reuse2709-60922709-61062024-03-01141809410.2166/wrd.2024.131131Evaluation of UVLED disinfection for biofouling control during distribution of wastewater effluentTara Randall0Inbar Shlomo1Emma Wells2Breanna Real3Ben Ma4Yarrow Linden5Jasmine Gamboa6Eran Friedler7Karl G. Linden8 Department of Civil, Environmental, and Architectural Engineering, University of Colorado Boulder, UCB 428, Boulder, CO 80309, USA Department of Environmental, Water & Agricultural Engineering, Faculty of Civil and Environmental Engineering, Technion–Israel Institute of Technology, Haifa 32000, Israel Department of Civil, Environmental, and Architectural Engineering, University of Colorado Boulder, UCB 428, Boulder, CO 80309, USA Department of Civil, Environmental, and Architectural Engineering, University of Colorado Boulder, UCB 428, Boulder, CO 80309, USA Department of Civil, Environmental, and Architectural Engineering, University of Colorado Boulder, UCB 428, Boulder, CO 80309, USA Department of Civil, Environmental, and Architectural Engineering, University of Colorado Boulder, UCB 428, Boulder, CO 80309, USA Department of Civil, Environmental, and Architectural Engineering, University of Colorado Boulder, UCB 428, Boulder, CO 80309, USA Department of Environmental, Water & Agricultural Engineering, Faculty of Civil and Environmental Engineering, Technion–Israel Institute of Technology, Haifa 32000, Israel Department of Civil, Environmental, and Architectural Engineering, University of Colorado Boulder, UCB 428, Boulder, CO 80309, USA A commercially available UVLED flow-through device, operating at 40 mJ/cm2, was examined for biofilm control on irrigation pipe material fed by wastewater effluent. Biofouling was monitored through total coliform counts, crystal violet (CV) staining, and ATP analyses. A UV fluence of 40 mJ/cm2 at 280 nm retarded biofilm formation; however, complete biofilm prevention by UV treatment was not achieved despite a high inactivation of planktonic cells. After 5 days of the study, the total coliform and CV biofilm quantification assays between the UV-treated and control bioreactor coupons were not statistically different. The total coliform counts indicated a stable biofilm cell concentration was reached; the CV assay showed biofilm biomass accumulation with time. The ATP results revealed higher coupon ATP on the UV-treated coupons than the control coupons by day 5. The results provoke an interesting discussion surrounding the contribution of viable cells, represented by total coliforms, and extracellular polymeric substance (EPS) to total biofilm biomass. This study also highlighted a need for further investigation into the relationship between ATP responses and complex UV-stress responses of diverse microbial communities as opposed to pure bacteria cultures. HIGHLIGHTS A UV fluence of 40 mJ/cm2 at 280 nm slows biofilm formation on irrigation pipe material.; Bioreactor effluent total coliform concentrations and crystal violet coupon accumulation were not statistically different between UV-treated and control bioreactors after being fed wastewater effluent for five days.; ATP results revealed higher ATP on UV-treated coupons than control coupons after 5 days in bioreactors fed by wastewater effluent.;http://wreuse.iwaponline.com/content/14/1/80biofilmbioreactorirrigationreuseuvwastewater |
| spellingShingle | Tara Randall Inbar Shlomo Emma Wells Breanna Real Ben Ma Yarrow Linden Jasmine Gamboa Eran Friedler Karl G. Linden Evaluation of UVLED disinfection for biofouling control during distribution of wastewater effluent Water Reuse biofilm bioreactor irrigation reuse uv wastewater |
| title | Evaluation of UVLED disinfection for biofouling control during distribution of wastewater effluent |
| title_full | Evaluation of UVLED disinfection for biofouling control during distribution of wastewater effluent |
| title_fullStr | Evaluation of UVLED disinfection for biofouling control during distribution of wastewater effluent |
| title_full_unstemmed | Evaluation of UVLED disinfection for biofouling control during distribution of wastewater effluent |
| title_short | Evaluation of UVLED disinfection for biofouling control during distribution of wastewater effluent |
| title_sort | evaluation of uvled disinfection for biofouling control during distribution of wastewater effluent |
| topic | biofilm bioreactor irrigation reuse uv wastewater |
| url | http://wreuse.iwaponline.com/content/14/1/80 |
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