Customizable 3D printed perfusion bioreactor for the engineering of stem cell microenvironments
Faithful modeling of tissues and organs requires the development of systems reflecting their dynamic 3D cellular architecture and organization. Current technologies suffer from a lack of design flexibility and complex prototyping, preventing their broad adoption by the scientific community. To make...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2022.1081145/full |
| _version_ | 1797957596446982144 |
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| author | Steven J. Dupard Steven J. Dupard Alejandro Garcia Garcia Alejandro Garcia Garcia Paul E. Bourgine Paul E. Bourgine |
| author_facet | Steven J. Dupard Steven J. Dupard Alejandro Garcia Garcia Alejandro Garcia Garcia Paul E. Bourgine Paul E. Bourgine |
| author_sort | Steven J. Dupard |
| collection | DOAJ |
| description | Faithful modeling of tissues and organs requires the development of systems reflecting their dynamic 3D cellular architecture and organization. Current technologies suffer from a lack of design flexibility and complex prototyping, preventing their broad adoption by the scientific community. To make 3D cell culture more available and adaptable we here describe the use of the fused deposition modeling (FDM) technology to rapid-prototype 3D printed perfusion bioreactors. Our 3D printed bioreactors are made of polylactic acid resulting in reusable systems customizable in size and shape. Following design confirmation, our bioreactors were biologically validated for the culture of human mesenchymal stromal cells under perfusion for up to 2 weeks on collagen scaffolds. Microenvironments of various size/volume (6–12 mm in diameter) could be engineered, by modulating the 3D printed bioreactor design. Metabolic assay and confocal microscopy confirmed the homogenous mesenchymal cell distribution throughout the material pores. The resulting human microenvironments were further exploited for the maintenance of human hematopoietic stem cells. Following 1 week of stromal coculture, we report the recapitulation of 3D interactions between the mesenchymal and hematopoietic fractions, associated with a phenotypic expansion of the blood stem cell populations.Our data confirm that perfusion bioreactors fit for cell culture can be generated using a 3D printing technology and exploited for the 3D modeling of complex stem cell systems. Our approach opens the gates for a more faithful investigation of cellular processes in relation to a dynamic 3D microenvironment. |
| first_indexed | 2024-04-11T00:07:36Z |
| format | Article |
| id | doaj.art-570792b68da74b259b7c7e7d3722b42b |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-11T00:07:36Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-570792b68da74b259b7c7e7d3722b42b2023-01-09T10:46:08ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852023-01-011010.3389/fbioe.2022.10811451081145Customizable 3D printed perfusion bioreactor for the engineering of stem cell microenvironmentsSteven J. Dupard0Steven J. Dupard1Alejandro Garcia Garcia2Alejandro Garcia Garcia3Paul E. Bourgine4Paul E. Bourgine5Cell, Tissue and Organ engineering laboratory, Biomedical Centre (BMC), Department of Clinical Sciences Lund, Stem Cell Centre, Lund University, Lund, SwedenWallenberg Centre for Molecular Medicine, Lund University, Lund, SwedenCell, Tissue and Organ engineering laboratory, Biomedical Centre (BMC), Department of Clinical Sciences Lund, Stem Cell Centre, Lund University, Lund, SwedenWallenberg Centre for Molecular Medicine, Lund University, Lund, SwedenCell, Tissue and Organ engineering laboratory, Biomedical Centre (BMC), Department of Clinical Sciences Lund, Stem Cell Centre, Lund University, Lund, SwedenWallenberg Centre for Molecular Medicine, Lund University, Lund, SwedenFaithful modeling of tissues and organs requires the development of systems reflecting their dynamic 3D cellular architecture and organization. Current technologies suffer from a lack of design flexibility and complex prototyping, preventing their broad adoption by the scientific community. To make 3D cell culture more available and adaptable we here describe the use of the fused deposition modeling (FDM) technology to rapid-prototype 3D printed perfusion bioreactors. Our 3D printed bioreactors are made of polylactic acid resulting in reusable systems customizable in size and shape. Following design confirmation, our bioreactors were biologically validated for the culture of human mesenchymal stromal cells under perfusion for up to 2 weeks on collagen scaffolds. Microenvironments of various size/volume (6–12 mm in diameter) could be engineered, by modulating the 3D printed bioreactor design. Metabolic assay and confocal microscopy confirmed the homogenous mesenchymal cell distribution throughout the material pores. The resulting human microenvironments were further exploited for the maintenance of human hematopoietic stem cells. Following 1 week of stromal coculture, we report the recapitulation of 3D interactions between the mesenchymal and hematopoietic fractions, associated with a phenotypic expansion of the blood stem cell populations.Our data confirm that perfusion bioreactors fit for cell culture can be generated using a 3D printing technology and exploited for the 3D modeling of complex stem cell systems. Our approach opens the gates for a more faithful investigation of cellular processes in relation to a dynamic 3D microenvironment.https://www.frontiersin.org/articles/10.3389/fbioe.2022.1081145/full3D printingpolylactic acidbioreactormesenchymal nichehematopoiesiscollagen scaffold |
| spellingShingle | Steven J. Dupard Steven J. Dupard Alejandro Garcia Garcia Alejandro Garcia Garcia Paul E. Bourgine Paul E. Bourgine Customizable 3D printed perfusion bioreactor for the engineering of stem cell microenvironments Frontiers in Bioengineering and Biotechnology 3D printing polylactic acid bioreactor mesenchymal niche hematopoiesis collagen scaffold |
| title | Customizable 3D printed perfusion bioreactor for the engineering of stem cell microenvironments |
| title_full | Customizable 3D printed perfusion bioreactor for the engineering of stem cell microenvironments |
| title_fullStr | Customizable 3D printed perfusion bioreactor for the engineering of stem cell microenvironments |
| title_full_unstemmed | Customizable 3D printed perfusion bioreactor for the engineering of stem cell microenvironments |
| title_short | Customizable 3D printed perfusion bioreactor for the engineering of stem cell microenvironments |
| title_sort | customizable 3d printed perfusion bioreactor for the engineering of stem cell microenvironments |
| topic | 3D printing polylactic acid bioreactor mesenchymal niche hematopoiesis collagen scaffold |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2022.1081145/full |
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