Gefitinib-loaded DSPE-PEG2000 nanomicelles with CD133 aptamers target lung cancer stem cells
Abstract Background Lung cancer stem cells (CSCs) are considered to be the seed of lung cancer, and CD133 is a marker of lung CSCs. Here, we developed gefitinib-loaded poly(ethylene glycol) 2000-distearoylphosphatidylethanolamine nanomicelles with CD133 aptamers (M-Gef-CD133) to eliminate CD133+ lun...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2017-08-01
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| Series: | World Journal of Surgical Oncology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12957-017-1230-4 |
| _version_ | 1798043761233625088 |
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| author | Xiaolong Huang Jingsong Huang Dewen Leng Shuo Yang Qi Yao Jin Sun Jun Hu |
| author_facet | Xiaolong Huang Jingsong Huang Dewen Leng Shuo Yang Qi Yao Jin Sun Jun Hu |
| author_sort | Xiaolong Huang |
| collection | DOAJ |
| description | Abstract Background Lung cancer stem cells (CSCs) are considered to be the seed of lung cancer, and CD133 is a marker of lung CSCs. Here, we developed gefitinib-loaded poly(ethylene glycol) 2000-distearoylphosphatidylethanolamine nanomicelles with CD133 aptamers (M-Gef-CD133) to eliminate CD133+ lung CSCs. Methods M-Gef-CD133 was prepared using a lipid-film-based approach. The targeting and activity of M-Gef-CD133 towards lung CSCs were evaluated. Results M-Gef-CD133 were small (25 nm) and showed enhanced cytotoxic effect towards CD133+ lung CSCs compared with non-targeted M-Gef and gefitinib. Notably, M-Gef-CD133 could significantly reduce tumor sphere formation and the percentage of CD133+ lung CSCs, indicating that it possesses selective toxicity against CD133+ lung CSCs. Conclusions The interaction of CD133 aptamers and CD133 shows promise in the delivery of gefitinib to CD133+ lung CSCs, and M-Gef-CD133 represents a promising treatment to target lung CSCs. |
| first_indexed | 2024-04-11T22:53:35Z |
| format | Article |
| id | doaj.art-570addf388f64151a3c3af5e9a00c332 |
| institution | Directory Open Access Journal |
| issn | 1477-7819 |
| language | English |
| last_indexed | 2024-04-11T22:53:35Z |
| publishDate | 2017-08-01 |
| publisher | BMC |
| record_format | Article |
| series | World Journal of Surgical Oncology |
| spelling | doaj.art-570addf388f64151a3c3af5e9a00c3322022-12-22T03:58:30ZengBMCWorld Journal of Surgical Oncology1477-78192017-08-0115111010.1186/s12957-017-1230-4Gefitinib-loaded DSPE-PEG2000 nanomicelles with CD133 aptamers target lung cancer stem cellsXiaolong Huang0Jingsong Huang1Dewen Leng2Shuo Yang3Qi Yao4Jin Sun5Jun Hu6Department of Respiratory Medicine, Wuhan NO. 1 HospitalLaboratory Medicine, Third Hubei Provincial People’s HospitalDepartment of Critical Care Medicine, Wuhan NO. 1 HospitalDepartment of Respiratory Medicine, Wuhan NO. 1 HospitalDepartment of Otolaryngology, Wuhan NO. 1 HospitalDepartment of Pharmacy, Second Military Medical UniversityDepartment of Physical examination, Wuhan Hospital for Occupational Disease Prevention and TreatmentAbstract Background Lung cancer stem cells (CSCs) are considered to be the seed of lung cancer, and CD133 is a marker of lung CSCs. Here, we developed gefitinib-loaded poly(ethylene glycol) 2000-distearoylphosphatidylethanolamine nanomicelles with CD133 aptamers (M-Gef-CD133) to eliminate CD133+ lung CSCs. Methods M-Gef-CD133 was prepared using a lipid-film-based approach. The targeting and activity of M-Gef-CD133 towards lung CSCs were evaluated. Results M-Gef-CD133 were small (25 nm) and showed enhanced cytotoxic effect towards CD133+ lung CSCs compared with non-targeted M-Gef and gefitinib. Notably, M-Gef-CD133 could significantly reduce tumor sphere formation and the percentage of CD133+ lung CSCs, indicating that it possesses selective toxicity against CD133+ lung CSCs. Conclusions The interaction of CD133 aptamers and CD133 shows promise in the delivery of gefitinib to CD133+ lung CSCs, and M-Gef-CD133 represents a promising treatment to target lung CSCs.http://link.springer.com/article/10.1186/s12957-017-1230-4NanomicellesLung cancerGefitinibCancer stem cellsCD133 |
| spellingShingle | Xiaolong Huang Jingsong Huang Dewen Leng Shuo Yang Qi Yao Jin Sun Jun Hu Gefitinib-loaded DSPE-PEG2000 nanomicelles with CD133 aptamers target lung cancer stem cells World Journal of Surgical Oncology Nanomicelles Lung cancer Gefitinib Cancer stem cells CD133 |
| title | Gefitinib-loaded DSPE-PEG2000 nanomicelles with CD133 aptamers target lung cancer stem cells |
| title_full | Gefitinib-loaded DSPE-PEG2000 nanomicelles with CD133 aptamers target lung cancer stem cells |
| title_fullStr | Gefitinib-loaded DSPE-PEG2000 nanomicelles with CD133 aptamers target lung cancer stem cells |
| title_full_unstemmed | Gefitinib-loaded DSPE-PEG2000 nanomicelles with CD133 aptamers target lung cancer stem cells |
| title_short | Gefitinib-loaded DSPE-PEG2000 nanomicelles with CD133 aptamers target lung cancer stem cells |
| title_sort | gefitinib loaded dspe peg2000 nanomicelles with cd133 aptamers target lung cancer stem cells |
| topic | Nanomicelles Lung cancer Gefitinib Cancer stem cells CD133 |
| url | http://link.springer.com/article/10.1186/s12957-017-1230-4 |
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