Paracapillaria (Ophidiocapillaria) siamensis sp. nov. (Nematoda: Trichuroidea): a new nematode in Naja kaouthia from Thailand
A comprehensive investigation, incorporating both morphological and molecular analyses, has unveiled the existence of a hitherto unknown nematode species, Paracapillaria (Ophidiocapillaria) siamensis sp. nov., residing in the intestine of the monocled cobra, Naja kaouthia, in the central region of T...
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| Format: | Article |
| Language: | English |
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Cambridge University Press
2024-04-01
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| Series: | Parasitology |
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| Online Access: | https://www.cambridge.org/core/product/identifier/S0031182024000404/type/journal_article |
| _version_ | 1827249857935441920 |
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| author | Vachirapong Charoennitiwat Kittipong Chaisiri Tapanee Kanjanapruthipong Sumate Ampawong Lawan Chanhome Taksa Vasaruchapong Urusa Thaenkham Napat Ratnarathorn |
| author_facet | Vachirapong Charoennitiwat Kittipong Chaisiri Tapanee Kanjanapruthipong Sumate Ampawong Lawan Chanhome Taksa Vasaruchapong Urusa Thaenkham Napat Ratnarathorn |
| author_sort | Vachirapong Charoennitiwat |
| collection | DOAJ |
| description | A comprehensive investigation, incorporating both morphological and molecular analyses, has unveiled the existence of a hitherto unknown nematode species, Paracapillaria (Ophidiocapillaria) siamensis sp. nov., residing in the intestine of the monocled cobra, Naja kaouthia, in the central region of Thailand. This study integrates morphological characteristics, morphometric examination, scanning electron microscopy and molecular phylogenetic analysis (COI, 18S rRNA and ITS1 genes). The findings place the newly described species within the subgenus Ophidiocapillaria, elucidating its distinctive characteristics, including a frame-like proximal spicule shape, approximate lengths of 19 000 and 22 500 μm with approximate widths of 90 and 130 μm for males and females, 39‒45 stichocytes, elevated lips without protrusion, a dorsal bacillary band stripe with an irregular pattern of bacillary cells and evidence of intestinal infection. These features serve to differentiate it from other species within the same subgenus, notably Paracapillaria (Ophidiocapillaria) najae De, 1998, a species coexisting P. siamensis sp. nov. in the monocled cobra from the same locality. This study addresses the co-infection of the novel species and P. najae within the same snake host, marking the second documented instance of a paracapillariid species in the monocled cobra within the family Elapidae. The genetic characterization supports the formal recognition of P. siamensis sp. nov. as a distinct species, thereby underscoring its taxonomic differentiation within the Capillariidae family. This research identifies and characterizes the new nematode species, contributing valuable insights into the taxonomy of this nematode. |
| first_indexed | 2024-04-24T13:47:04Z |
| format | Article |
| id | doaj.art-570da3f435ac4da7838ae3ced2fabb23 |
| institution | Directory Open Access Journal |
| issn | 0031-1820 1469-8161 |
| language | English |
| last_indexed | 2025-03-21T23:54:19Z |
| publishDate | 2024-04-01 |
| publisher | Cambridge University Press |
| record_format | Article |
| series | Parasitology |
| spelling | doaj.art-570da3f435ac4da7838ae3ced2fabb232024-05-17T10:08:15ZengCambridge University PressParasitology0031-18201469-81612024-04-0115152953810.1017/S0031182024000404Paracapillaria (Ophidiocapillaria) siamensis sp. nov. (Nematoda: Trichuroidea): a new nematode in Naja kaouthia from ThailandVachirapong Charoennitiwat0https://orcid.org/0000-0002-6367-6687Kittipong Chaisiri1https://orcid.org/0000-0001-6795-3541Tapanee Kanjanapruthipong2Sumate Ampawong3https://orcid.org/0000-0002-5378-2996Lawan Chanhome4https://orcid.org/0000-0003-2425-5000Taksa Vasaruchapong5https://orcid.org/0000-0001-8197-0356Urusa Thaenkham6https://orcid.org/0000-0003-0688-2422Napat Ratnarathorn7https://orcid.org/0000-0002-4894-5148Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok, ThailandDepartment of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok, ThailandDepartment of Tropical Pathology, Faculty of Tropical Medicine, Mahidol University, Bangkok, ThailandDepartment of Tropical Pathology, Faculty of Tropical Medicine, Mahidol University, Bangkok, ThailandSnake Farm, Queen Saovabha Memorial Institute, The Thai Red Cross Society, Bangkok, ThailandSnake Farm, Queen Saovabha Memorial Institute, The Thai Red Cross Society, Bangkok, ThailandDepartment of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok, ThailandAnimal Systematics & Molecular Ecology Laboratory and Applied Animal Science Laboratory, Department of Biology, Faculty of Science, Mahidol University, Bangkok, ThailandA comprehensive investigation, incorporating both morphological and molecular analyses, has unveiled the existence of a hitherto unknown nematode species, Paracapillaria (Ophidiocapillaria) siamensis sp. nov., residing in the intestine of the monocled cobra, Naja kaouthia, in the central region of Thailand. This study integrates morphological characteristics, morphometric examination, scanning electron microscopy and molecular phylogenetic analysis (COI, 18S rRNA and ITS1 genes). The findings place the newly described species within the subgenus Ophidiocapillaria, elucidating its distinctive characteristics, including a frame-like proximal spicule shape, approximate lengths of 19 000 and 22 500 μm with approximate widths of 90 and 130 μm for males and females, 39‒45 stichocytes, elevated lips without protrusion, a dorsal bacillary band stripe with an irregular pattern of bacillary cells and evidence of intestinal infection. These features serve to differentiate it from other species within the same subgenus, notably Paracapillaria (Ophidiocapillaria) najae De, 1998, a species coexisting P. siamensis sp. nov. in the monocled cobra from the same locality. This study addresses the co-infection of the novel species and P. najae within the same snake host, marking the second documented instance of a paracapillariid species in the monocled cobra within the family Elapidae. The genetic characterization supports the formal recognition of P. siamensis sp. nov. as a distinct species, thereby underscoring its taxonomic differentiation within the Capillariidae family. This research identifies and characterizes the new nematode species, contributing valuable insights into the taxonomy of this nematode.https://www.cambridge.org/core/product/identifier/S0031182024000404/type/journal_articlegastrointestinal helminthmonocled cobramorphological and molecular analysisNaja kaouthiaOphidiocapillariaParacapillaria siamensis |
| spellingShingle | Vachirapong Charoennitiwat Kittipong Chaisiri Tapanee Kanjanapruthipong Sumate Ampawong Lawan Chanhome Taksa Vasaruchapong Urusa Thaenkham Napat Ratnarathorn Paracapillaria (Ophidiocapillaria) siamensis sp. nov. (Nematoda: Trichuroidea): a new nematode in Naja kaouthia from Thailand Parasitology gastrointestinal helminth monocled cobra morphological and molecular analysis Naja kaouthia Ophidiocapillaria Paracapillaria siamensis |
| title | Paracapillaria (Ophidiocapillaria) siamensis sp. nov. (Nematoda: Trichuroidea): a new nematode in Naja kaouthia from Thailand |
| title_full | Paracapillaria (Ophidiocapillaria) siamensis sp. nov. (Nematoda: Trichuroidea): a new nematode in Naja kaouthia from Thailand |
| title_fullStr | Paracapillaria (Ophidiocapillaria) siamensis sp. nov. (Nematoda: Trichuroidea): a new nematode in Naja kaouthia from Thailand |
| title_full_unstemmed | Paracapillaria (Ophidiocapillaria) siamensis sp. nov. (Nematoda: Trichuroidea): a new nematode in Naja kaouthia from Thailand |
| title_short | Paracapillaria (Ophidiocapillaria) siamensis sp. nov. (Nematoda: Trichuroidea): a new nematode in Naja kaouthia from Thailand |
| title_sort | paracapillaria ophidiocapillaria siamensis sp nov nematoda trichuroidea a new nematode in naja kaouthia from thailand |
| topic | gastrointestinal helminth monocled cobra morphological and molecular analysis Naja kaouthia Ophidiocapillaria Paracapillaria siamensis |
| url | https://www.cambridge.org/core/product/identifier/S0031182024000404/type/journal_article |
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