Polyhydroxyalkanoates (PHAs) Production Through Conversion of Glycerol by Selected Strains of Pseudomonas Mediterranea and Pseudomonas Corrugata

Polyhydroxyalkanoates (PHAs) are polyesters synthesized by numerous bacteria as an intracellular carbon and energy storage compounds in the cytoplasm of cells. To cut down the cost of production and optimize the PHA/dried cell weight different concentrations (1, 2 and 5 %) of refined glycerol and a...

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Main Authors: R. Palmeri, F. Pappalardo, M. Fragala, M. Tomasello, A. Damigella, A. Catara
Format: Article
Language:English
Published: AIDIC Servizi S.r.l. 2012-06-01
Series:Chemical Engineering Transactions
Online Access:https://www.cetjournal.it/index.php/cet/article/view/7336
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author R. Palmeri
F. Pappalardo
M. Fragala
M. Tomasello
A. Damigella
A. Catara
author_facet R. Palmeri
F. Pappalardo
M. Fragala
M. Tomasello
A. Damigella
A. Catara
author_sort R. Palmeri
collection DOAJ
description Polyhydroxyalkanoates (PHAs) are polyesters synthesized by numerous bacteria as an intracellular carbon and energy storage compounds in the cytoplasm of cells. To cut down the cost of production and optimize the PHA/dried cell weight different concentrations (1, 2 and 5 %) of refined glycerol and a crude glycerol from biodiesel process, were used as carbon source in a batch fermentation process. Pseudomonas mediterranea 9.1 as well as Pseudomonas corrugata 388 and A1 reference strains, grew and synthesized a medium-chain-length poly(3-hy-droxyalkanoates) elastomer both on crude and refined glycerol carbon sources. The mcl-PHA produced by P. mediterranea 9.1 grown on refined glycerol was apparently different as a result of a different metabolic pathway. Gas chromatography PHA’s Different monomeric units of side chains, which ranged from C12 to C19 in length in mcl-PHA(CG) and from C5 to C16 in mcl-PHA(RG) were obtained.
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spelling doaj.art-5712d419e97b4e0b97870a8e0a45f1382022-12-21T20:33:19ZengAIDIC Servizi S.r.l.Chemical Engineering Transactions2283-92162012-06-012710.3303/CET1227021Polyhydroxyalkanoates (PHAs) Production Through Conversion of Glycerol by Selected Strains of Pseudomonas Mediterranea and Pseudomonas CorrugataR. PalmeriF. PappalardoM. FragalaM. TomaselloA. DamigellaA. CataraPolyhydroxyalkanoates (PHAs) are polyesters synthesized by numerous bacteria as an intracellular carbon and energy storage compounds in the cytoplasm of cells. To cut down the cost of production and optimize the PHA/dried cell weight different concentrations (1, 2 and 5 %) of refined glycerol and a crude glycerol from biodiesel process, were used as carbon source in a batch fermentation process. Pseudomonas mediterranea 9.1 as well as Pseudomonas corrugata 388 and A1 reference strains, grew and synthesized a medium-chain-length poly(3-hy-droxyalkanoates) elastomer both on crude and refined glycerol carbon sources. The mcl-PHA produced by P. mediterranea 9.1 grown on refined glycerol was apparently different as a result of a different metabolic pathway. Gas chromatography PHA’s Different monomeric units of side chains, which ranged from C12 to C19 in length in mcl-PHA(CG) and from C5 to C16 in mcl-PHA(RG) were obtained.https://www.cetjournal.it/index.php/cet/article/view/7336
spellingShingle R. Palmeri
F. Pappalardo
M. Fragala
M. Tomasello
A. Damigella
A. Catara
Polyhydroxyalkanoates (PHAs) Production Through Conversion of Glycerol by Selected Strains of Pseudomonas Mediterranea and Pseudomonas Corrugata
Chemical Engineering Transactions
title Polyhydroxyalkanoates (PHAs) Production Through Conversion of Glycerol by Selected Strains of Pseudomonas Mediterranea and Pseudomonas Corrugata
title_full Polyhydroxyalkanoates (PHAs) Production Through Conversion of Glycerol by Selected Strains of Pseudomonas Mediterranea and Pseudomonas Corrugata
title_fullStr Polyhydroxyalkanoates (PHAs) Production Through Conversion of Glycerol by Selected Strains of Pseudomonas Mediterranea and Pseudomonas Corrugata
title_full_unstemmed Polyhydroxyalkanoates (PHAs) Production Through Conversion of Glycerol by Selected Strains of Pseudomonas Mediterranea and Pseudomonas Corrugata
title_short Polyhydroxyalkanoates (PHAs) Production Through Conversion of Glycerol by Selected Strains of Pseudomonas Mediterranea and Pseudomonas Corrugata
title_sort polyhydroxyalkanoates phas production through conversion of glycerol by selected strains of pseudomonas mediterranea and pseudomonas corrugata
url https://www.cetjournal.it/index.php/cet/article/view/7336
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