Modular Assembly of Cell-targeting Devices Based on an Uncommon G-quadruplex Aptamer
Aptamers are valuable tools that provide great potential to develop cost-effective diagnostics and therapies in the biomedical field. Here, we report a novel DNA aptamer that folds into an unconventional G-quadruplex structure able to recognize and enter specifically into human Burkitt's lympho...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2015-01-01
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| Series: | Molecular Therapy: Nucleic Acids |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2162253116300361 |
| _version_ | 1828420560346939392 |
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| author | Felipe Opazo Laura Eiden Line Hansen Falk Rohrbach Jesper Wengel Jørgen Kjems Günter Mayer |
| author_facet | Felipe Opazo Laura Eiden Line Hansen Falk Rohrbach Jesper Wengel Jørgen Kjems Günter Mayer |
| author_sort | Felipe Opazo |
| collection | DOAJ |
| description | Aptamers are valuable tools that provide great potential to develop cost-effective diagnostics and therapies in the biomedical field. Here, we report a novel DNA aptamer that folds into an unconventional G-quadruplex structure able to recognize and enter specifically into human Burkitt's lymphoma cells. We further optimized this aptamer to a highly versatile and stable minimized version. The minimized aptamer can be easily equipped with different functionalities like quantum dots, organic dyes, or even a second different aptamer domain yielding a bi-paratopic aptamer. Although the target molecule of the aptamer remains unknown, our microscopy and pharmacological studies revealed that the aptamer hijacks the clathrin-mediated endocytosis pathway for its cellular internalization. We conclude that this novel class of aptamers can be used as a modular tool to specifically deliver different cargoes into malignant cells. This work provides a thorough characterization of the aptamer and we expect that our strategy will pave the path for future therapeutic applications. |
| first_indexed | 2024-12-10T15:14:09Z |
| format | Article |
| id | doaj.art-57184635e6e44f0cab1bd65a29a26d76 |
| institution | Directory Open Access Journal |
| issn | 2162-2531 |
| language | English |
| last_indexed | 2024-12-10T15:14:09Z |
| publishDate | 2015-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Molecular Therapy: Nucleic Acids |
| spelling | doaj.art-57184635e6e44f0cab1bd65a29a26d762022-12-22T01:43:50ZengElsevierMolecular Therapy: Nucleic Acids2162-25312015-01-014C10.1038/mtna.2015.25Modular Assembly of Cell-targeting Devices Based on an Uncommon G-quadruplex AptamerFelipe Opazo0Laura Eiden1Line Hansen2Falk Rohrbach3Jesper Wengel4Jørgen Kjems5Günter Mayer6Department of Neuro- and Sensory Physiology, University of Göttingen Medical Center, Göttingen, GermanyLife and Medical Sciences Institute, University of Bonn, Bonn, GermanyInterdisciplinary Nanoscience Center (iNANO), Aarhus University, Aarhus C, DenmarkLife and Medical Sciences Institute, University of Bonn, Bonn, GermanyNucleic Acid Center, University of Southern Denmark, Odense M, DenmarkInterdisciplinary Nanoscience Center (iNANO), Aarhus University, Aarhus C, DenmarkLife and Medical Sciences Institute, University of Bonn, Bonn, GermanyAptamers are valuable tools that provide great potential to develop cost-effective diagnostics and therapies in the biomedical field. Here, we report a novel DNA aptamer that folds into an unconventional G-quadruplex structure able to recognize and enter specifically into human Burkitt's lymphoma cells. We further optimized this aptamer to a highly versatile and stable minimized version. The minimized aptamer can be easily equipped with different functionalities like quantum dots, organic dyes, or even a second different aptamer domain yielding a bi-paratopic aptamer. Although the target molecule of the aptamer remains unknown, our microscopy and pharmacological studies revealed that the aptamer hijacks the clathrin-mediated endocytosis pathway for its cellular internalization. We conclude that this novel class of aptamers can be used as a modular tool to specifically deliver different cargoes into malignant cells. This work provides a thorough characterization of the aptamer and we expect that our strategy will pave the path for future therapeutic applications.http://www.sciencedirect.com/science/article/pii/S2162253116300361AptamersG-cuadruplexBurkitt´s lymphomaRAMOSbi-paratopic |
| spellingShingle | Felipe Opazo Laura Eiden Line Hansen Falk Rohrbach Jesper Wengel Jørgen Kjems Günter Mayer Modular Assembly of Cell-targeting Devices Based on an Uncommon G-quadruplex Aptamer Molecular Therapy: Nucleic Acids Aptamers G-cuadruplex Burkitt´s lymphoma RAMOS bi-paratopic |
| title | Modular Assembly of Cell-targeting Devices Based on an Uncommon G-quadruplex Aptamer |
| title_full | Modular Assembly of Cell-targeting Devices Based on an Uncommon G-quadruplex Aptamer |
| title_fullStr | Modular Assembly of Cell-targeting Devices Based on an Uncommon G-quadruplex Aptamer |
| title_full_unstemmed | Modular Assembly of Cell-targeting Devices Based on an Uncommon G-quadruplex Aptamer |
| title_short | Modular Assembly of Cell-targeting Devices Based on an Uncommon G-quadruplex Aptamer |
| title_sort | modular assembly of cell targeting devices based on an uncommon g quadruplex aptamer |
| topic | Aptamers G-cuadruplex Burkitt´s lymphoma RAMOS bi-paratopic |
| url | http://www.sciencedirect.com/science/article/pii/S2162253116300361 |
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