Typing of the Gut Microbiota Community in Japanese Subjects
Gut microbiota are involved in both host health and disease and can be stratified based on bacteriological composition. However, gut microbiota clustering data are limited for Asians. In this study, fecal microbiota of 1803 Japanese subjects, including 283 healthy individuals, were analyzed by 16S r...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/3/664 |
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| author | Tomohisa Takagi Ryo Inoue Akira Oshima Hiroshi Sakazume Kenta Ogawa Tomo Tominaga Yoichi Mihara Takeshi Sugaya Katsura Mizushima Kazuhiko Uchiyama Yoshito Itoh Yuji Naito |
| author_facet | Tomohisa Takagi Ryo Inoue Akira Oshima Hiroshi Sakazume Kenta Ogawa Tomo Tominaga Yoichi Mihara Takeshi Sugaya Katsura Mizushima Kazuhiko Uchiyama Yoshito Itoh Yuji Naito |
| author_sort | Tomohisa Takagi |
| collection | DOAJ |
| description | Gut microbiota are involved in both host health and disease and can be stratified based on bacteriological composition. However, gut microbiota clustering data are limited for Asians. In this study, fecal microbiota of 1803 Japanese subjects, including 283 healthy individuals, were analyzed by 16S rRNA sequencing and clustered using two models. The association of various diseases with each community type was also assessed. Five and fifteen communities were identified using partitioning around medoids (PAM) and the Dirichlet multinominal mixtures model, respectively. Bacteria exhibiting characteristically high abundance among the PAM-identified types were of the family <i>Ruminococcaceae</i> (Type A) and genera <i>Bacteroides</i>, <i>Blautia</i>, and <i>Faecalibacterium</i> (Type B); <i>Bacteroides</i>, <i>Fusobacterium</i>, and <i>Proteus</i> (Type C); and <i>Bifidobacterium</i> (Type D), and <i>Prevotella</i> (Type E). The most noteworthy community found in the Japanese subjects was the <i>Bifidobacterium</i>-rich community. The odds ratio based on type E, which had the largest population of healthy subjects, revealed that other types (especially types A, C, and D) were highly associated with various diseases, including inflammatory bowel disease, functional gastrointestinal disorder, and lifestyle-related diseases. Gut microbiota community typing reproducibly identified organisms that may represent enterotypes peculiar to Japanese individuals and that are partly different from those of indivuals from Western countries. |
| first_indexed | 2024-03-09T13:14:28Z |
| format | Article |
| id | doaj.art-5718e06d79cc4afab74793ccb0e1c348 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T13:14:28Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-5718e06d79cc4afab74793ccb0e1c3482023-11-30T21:37:45ZengMDPI AGMicroorganisms2076-26072022-03-0110366410.3390/microorganisms10030664Typing of the Gut Microbiota Community in Japanese SubjectsTomohisa Takagi0Ryo Inoue1Akira Oshima2Hiroshi Sakazume3Kenta Ogawa4Tomo Tominaga5Yoichi Mihara6Takeshi Sugaya7Katsura Mizushima8Kazuhiko Uchiyama9Yoshito Itoh10Yuji Naito11Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, JapanLaboratory of Animal Science, Setsunan University, Osaka 573-0101, JapanDepartment of Research and Development, PreMedica Inc., Tokyo 105-0011, JapanDepartment of Research and Development, PreMedica Inc., Tokyo 105-0011, JapanDepartment of Research and Development, PreMedica Inc., Tokyo 105-0011, JapanDepartment of Research and Development, PreMedica Inc., Tokyo 105-0011, JapanDepartment of Research and Development, PreMedica Inc., Tokyo 105-0011, JapanMolecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, JapanDepartment of Human Immunology and Nutrition Science, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, JapanMolecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, JapanMolecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, JapanDepartment of Human Immunology and Nutrition Science, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, JapanGut microbiota are involved in both host health and disease and can be stratified based on bacteriological composition. However, gut microbiota clustering data are limited for Asians. In this study, fecal microbiota of 1803 Japanese subjects, including 283 healthy individuals, were analyzed by 16S rRNA sequencing and clustered using two models. The association of various diseases with each community type was also assessed. Five and fifteen communities were identified using partitioning around medoids (PAM) and the Dirichlet multinominal mixtures model, respectively. Bacteria exhibiting characteristically high abundance among the PAM-identified types were of the family <i>Ruminococcaceae</i> (Type A) and genera <i>Bacteroides</i>, <i>Blautia</i>, and <i>Faecalibacterium</i> (Type B); <i>Bacteroides</i>, <i>Fusobacterium</i>, and <i>Proteus</i> (Type C); and <i>Bifidobacterium</i> (Type D), and <i>Prevotella</i> (Type E). The most noteworthy community found in the Japanese subjects was the <i>Bifidobacterium</i>-rich community. The odds ratio based on type E, which had the largest population of healthy subjects, revealed that other types (especially types A, C, and D) were highly associated with various diseases, including inflammatory bowel disease, functional gastrointestinal disorder, and lifestyle-related diseases. Gut microbiota community typing reproducibly identified organisms that may represent enterotypes peculiar to Japanese individuals and that are partly different from those of indivuals from Western countries.https://www.mdpi.com/2076-2607/10/3/664gut microbiota communitypartitioning around medoids (PAM) modelDirichlet multinominal mixtures (DMM) model<i>Bifidobacterium</i>enterotype |
| spellingShingle | Tomohisa Takagi Ryo Inoue Akira Oshima Hiroshi Sakazume Kenta Ogawa Tomo Tominaga Yoichi Mihara Takeshi Sugaya Katsura Mizushima Kazuhiko Uchiyama Yoshito Itoh Yuji Naito Typing of the Gut Microbiota Community in Japanese Subjects Microorganisms gut microbiota community partitioning around medoids (PAM) model Dirichlet multinominal mixtures (DMM) model <i>Bifidobacterium</i> enterotype |
| title | Typing of the Gut Microbiota Community in Japanese Subjects |
| title_full | Typing of the Gut Microbiota Community in Japanese Subjects |
| title_fullStr | Typing of the Gut Microbiota Community in Japanese Subjects |
| title_full_unstemmed | Typing of the Gut Microbiota Community in Japanese Subjects |
| title_short | Typing of the Gut Microbiota Community in Japanese Subjects |
| title_sort | typing of the gut microbiota community in japanese subjects |
| topic | gut microbiota community partitioning around medoids (PAM) model Dirichlet multinominal mixtures (DMM) model <i>Bifidobacterium</i> enterotype |
| url | https://www.mdpi.com/2076-2607/10/3/664 |
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