Isolation, characterization and performance of extra cellular polymer substances (EPS) producing bacteria from biofloc culture water of Nile tilapia using distillery spentwash as carbon source
The present study aimed to isolate and characterize the Extracellular polymeric substance (EPS) producing bacteria from biofloc reared Nile tilapia (Chitralada) ponds. Distillery spentwash was used as a carbon source to maintain the C: N ratio at 10: 1 in the fish culture ponds and screening of bact...
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| Format: | Article |
| Language: | English |
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Indian Council of Agricultural Research
2020-09-01
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| Series: | Indian Journal of Animal Sciences |
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| Online Access: | https://epubs.icar.org.in/index.php/IJAnS/article/view/104640 |
| _version_ | 1797896810676617216 |
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| author | M MENAGA S FELIX C MOHANASUNDARI M CHARULATHA |
| author_facet | M MENAGA S FELIX C MOHANASUNDARI M CHARULATHA |
| author_sort | M MENAGA |
| collection | DOAJ |
| description | The present study aimed to isolate and characterize the Extracellular polymeric substance (EPS) producing bacteria from biofloc reared Nile tilapia (Chitralada) ponds. Distillery spentwash was used as a carbon source to maintain the C: N ratio at 10: 1 in the fish culture ponds and screening of bacteria were done fortnightly in 180 days culture. Out of 38 bacterial isolates, 7 isolates were found to produce EPS. Based on 16s rRNA sequence analysis the isolates were identified as Bacillus subtilis, B. megaterium, B. infantis, B. cereus, Pseudomonas balearica, P. mendocina and P. alcaligenes. The highest production of EPS was recorded in B. cereus (1.25 g/L). EPS extracted from Bacillus cereus was reported to have higher protein (89 μg/ml) and B. subtilis possessed higher carbohydrate (753.75 μg/ml). Maximum flocculating ability of 40.18% in B. cereus and higher emulsifying activity of 63.53% was observed in B. megaterium. The EPS extracted from B. infantis showed lower sludge volume index on its treatment with aquaculture sludge (15.38 ml/g). Absorption band in the range of 4,000/cm to 450/cm using FTIR analysis confirmed the presence of characteristic functional bands arising from polysaccharides, nucleic acids and proteins. The results indicated the presence of EPS producing bacteria in biofloc based Nile tilapia aquaculture systems. |
| first_indexed | 2024-04-10T07:48:44Z |
| format | Article |
| id | doaj.art-571a200bd8154f1c91c9d96623e5a539 |
| institution | Directory Open Access Journal |
| issn | 0367-8318 2394-3327 |
| language | English |
| last_indexed | 2024-04-10T07:48:44Z |
| publishDate | 2020-09-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | Indian Journal of Animal Sciences |
| spelling | doaj.art-571a200bd8154f1c91c9d96623e5a5392023-02-23T10:24:09ZengIndian Council of Agricultural ResearchIndian Journal of Animal Sciences0367-83182394-33272020-09-0190510.56093/ijans.v90i5.104640Isolation, characterization and performance of extra cellular polymer substances (EPS) producing bacteria from biofloc culture water of Nile tilapia using distillery spentwash as carbon sourceM MENAGA0S FELIX1C MOHANASUNDARI2M CHARULATHA3Dr MGR Fisheries College and Research Institute, Madhavaram Milk Colony, Chennai, Tamil Nadu 600 051 IndiaDr MGR Fisheries College and Research Institute, Madhavaram Milk Colony, Chennai, Tamil Nadu 600 051 IndiaDr MGR Fisheries College and Research Institute, Madhavaram Milk Colony, Chennai, Tamil Nadu 600 051 IndiaDr MGR Fisheries College and Research Institute, Madhavaram Milk Colony, Chennai, Tamil Nadu 600 051 IndiaThe present study aimed to isolate and characterize the Extracellular polymeric substance (EPS) producing bacteria from biofloc reared Nile tilapia (Chitralada) ponds. Distillery spentwash was used as a carbon source to maintain the C: N ratio at 10: 1 in the fish culture ponds and screening of bacteria were done fortnightly in 180 days culture. Out of 38 bacterial isolates, 7 isolates were found to produce EPS. Based on 16s rRNA sequence analysis the isolates were identified as Bacillus subtilis, B. megaterium, B. infantis, B. cereus, Pseudomonas balearica, P. mendocina and P. alcaligenes. The highest production of EPS was recorded in B. cereus (1.25 g/L). EPS extracted from Bacillus cereus was reported to have higher protein (89 μg/ml) and B. subtilis possessed higher carbohydrate (753.75 μg/ml). Maximum flocculating ability of 40.18% in B. cereus and higher emulsifying activity of 63.53% was observed in B. megaterium. The EPS extracted from B. infantis showed lower sludge volume index on its treatment with aquaculture sludge (15.38 ml/g). Absorption band in the range of 4,000/cm to 450/cm using FTIR analysis confirmed the presence of characteristic functional bands arising from polysaccharides, nucleic acids and proteins. The results indicated the presence of EPS producing bacteria in biofloc based Nile tilapia aquaculture systems.https://epubs.icar.org.in/index.php/IJAnS/article/view/104640BioflocExtracellular polymeric substancesFlocculationFTIR |
| spellingShingle | M MENAGA S FELIX C MOHANASUNDARI M CHARULATHA Isolation, characterization and performance of extra cellular polymer substances (EPS) producing bacteria from biofloc culture water of Nile tilapia using distillery spentwash as carbon source Indian Journal of Animal Sciences Biofloc Extracellular polymeric substances Flocculation FTIR |
| title | Isolation, characterization and performance of extra cellular polymer substances (EPS) producing bacteria from biofloc culture water of Nile tilapia using distillery spentwash as carbon source |
| title_full | Isolation, characterization and performance of extra cellular polymer substances (EPS) producing bacteria from biofloc culture water of Nile tilapia using distillery spentwash as carbon source |
| title_fullStr | Isolation, characterization and performance of extra cellular polymer substances (EPS) producing bacteria from biofloc culture water of Nile tilapia using distillery spentwash as carbon source |
| title_full_unstemmed | Isolation, characterization and performance of extra cellular polymer substances (EPS) producing bacteria from biofloc culture water of Nile tilapia using distillery spentwash as carbon source |
| title_short | Isolation, characterization and performance of extra cellular polymer substances (EPS) producing bacteria from biofloc culture water of Nile tilapia using distillery spentwash as carbon source |
| title_sort | isolation characterization and performance of extra cellular polymer substances eps producing bacteria from biofloc culture water of nile tilapia using distillery spentwash as carbon source |
| topic | Biofloc Extracellular polymeric substances Flocculation FTIR |
| url | https://epubs.icar.org.in/index.php/IJAnS/article/view/104640 |
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