Coupled Adsorption and Biodegradation of Trichloroethylene on Biochar from Pine Wood Wastes: A Combined Approach for a Sustainable Bioremediation Strategy
Towards chlorinated solvents, the effectiveness of the remediation strategy can be improved by combining a biological approach (e.g., anaerobic reductive dechlorination) with chemical/physical treatments (e.g., adsorption). A coupled adsorption and biodegradation (CAB) process for trichloroethylene...
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MDPI AG
2022-01-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/1/101 |
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| author | Marta M. Rossi Bruna Matturro Neda Amanat Simona Rossetti Marco Petrangeli Papini |
| author_facet | Marta M. Rossi Bruna Matturro Neda Amanat Simona Rossetti Marco Petrangeli Papini |
| author_sort | Marta M. Rossi |
| collection | DOAJ |
| description | Towards chlorinated solvents, the effectiveness of the remediation strategy can be improved by combining a biological approach (e.g., anaerobic reductive dechlorination) with chemical/physical treatments (e.g., adsorption). A coupled adsorption and biodegradation (CAB) process for trichloroethylene (TCE) removal is proposed in a biofilm–biochar reactor (BBR) to assess whether biochar from pine wood (PWB) can support a dechlorinating biofilm by combining the TCE (100 µM) adsorption. The BBR operated for eight months in parallel with a biofilm reactor (BR)—no PWB (biological process alone), and with an abiotic biochar reactor (ABR)—no dechlorinating biofilm (only an adsorption mechanism). Two flow rates were investigated. Compared to the BR, which resulted in a TCE removal of 86.9 ± 11.9% and 78.73 ± 19.79%, the BBR demonstrated that PWB effectively adsorbs TCE and slows down the release of its intermediates. The elimination of TCE was quantitative, with 99.61 ± 0.79% and 99.87 ± 0.51% TCE removal. Interestingly, the biomarker of the reductive dechlorination process, <i>Dehalococcoides mccartyi</i>, was found in the BRR (9.2 × 10<sup>5</sup> 16S rRNA gene copies/g), together with the specific genes <i>tceA</i>, <i>bvcA</i>, and <i>vcrA</i> (8.16 × 10<sup>6</sup>, 1.28 × 10<sup>5</sup>, and 8.01 × 10<sup>3</sup> gene copies/g, respectively). This study suggests the feasibility of biochar to support the reductive dechlorination of <i>D. mccartyi</i>, opening new frontiers for field-scale applications. |
| first_indexed | 2024-03-10T00:53:44Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T00:53:44Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-571d6800b80a49b0b018991c8c25f7c92023-11-23T14:47:20ZengMDPI AGMicroorganisms2076-26072022-01-0110110110.3390/microorganisms10010101Coupled Adsorption and Biodegradation of Trichloroethylene on Biochar from Pine Wood Wastes: A Combined Approach for a Sustainable Bioremediation StrategyMarta M. Rossi0Bruna Matturro1Neda Amanat2Simona Rossetti3Marco Petrangeli Papini4Department of Chemistry, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, ItalyWater Research Institute (IRSA—CNR), Via Salaria km 29.300, 00015 Monterotondo, ItalyDepartment of Chemistry, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, ItalyWater Research Institute (IRSA—CNR), Via Salaria km 29.300, 00015 Monterotondo, ItalyDepartment of Chemistry, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, ItalyTowards chlorinated solvents, the effectiveness of the remediation strategy can be improved by combining a biological approach (e.g., anaerobic reductive dechlorination) with chemical/physical treatments (e.g., adsorption). A coupled adsorption and biodegradation (CAB) process for trichloroethylene (TCE) removal is proposed in a biofilm–biochar reactor (BBR) to assess whether biochar from pine wood (PWB) can support a dechlorinating biofilm by combining the TCE (100 µM) adsorption. The BBR operated for eight months in parallel with a biofilm reactor (BR)—no PWB (biological process alone), and with an abiotic biochar reactor (ABR)—no dechlorinating biofilm (only an adsorption mechanism). Two flow rates were investigated. Compared to the BR, which resulted in a TCE removal of 86.9 ± 11.9% and 78.73 ± 19.79%, the BBR demonstrated that PWB effectively adsorbs TCE and slows down the release of its intermediates. The elimination of TCE was quantitative, with 99.61 ± 0.79% and 99.87 ± 0.51% TCE removal. Interestingly, the biomarker of the reductive dechlorination process, <i>Dehalococcoides mccartyi</i>, was found in the BRR (9.2 × 10<sup>5</sup> 16S rRNA gene copies/g), together with the specific genes <i>tceA</i>, <i>bvcA</i>, and <i>vcrA</i> (8.16 × 10<sup>6</sup>, 1.28 × 10<sup>5</sup>, and 8.01 × 10<sup>3</sup> gene copies/g, respectively). This study suggests the feasibility of biochar to support the reductive dechlorination of <i>D. mccartyi</i>, opening new frontiers for field-scale applications.https://www.mdpi.com/2076-2607/10/1/101bioremediationadsorptionbiocharbiodegradationreductive dechlorination<i>Dehalococcoides mccartyi</i> |
| spellingShingle | Marta M. Rossi Bruna Matturro Neda Amanat Simona Rossetti Marco Petrangeli Papini Coupled Adsorption and Biodegradation of Trichloroethylene on Biochar from Pine Wood Wastes: A Combined Approach for a Sustainable Bioremediation Strategy Microorganisms bioremediation adsorption biochar biodegradation reductive dechlorination <i>Dehalococcoides mccartyi</i> |
| title | Coupled Adsorption and Biodegradation of Trichloroethylene on Biochar from Pine Wood Wastes: A Combined Approach for a Sustainable Bioremediation Strategy |
| title_full | Coupled Adsorption and Biodegradation of Trichloroethylene on Biochar from Pine Wood Wastes: A Combined Approach for a Sustainable Bioremediation Strategy |
| title_fullStr | Coupled Adsorption and Biodegradation of Trichloroethylene on Biochar from Pine Wood Wastes: A Combined Approach for a Sustainable Bioremediation Strategy |
| title_full_unstemmed | Coupled Adsorption and Biodegradation of Trichloroethylene on Biochar from Pine Wood Wastes: A Combined Approach for a Sustainable Bioremediation Strategy |
| title_short | Coupled Adsorption and Biodegradation of Trichloroethylene on Biochar from Pine Wood Wastes: A Combined Approach for a Sustainable Bioremediation Strategy |
| title_sort | coupled adsorption and biodegradation of trichloroethylene on biochar from pine wood wastes a combined approach for a sustainable bioremediation strategy |
| topic | bioremediation adsorption biochar biodegradation reductive dechlorination <i>Dehalococcoides mccartyi</i> |
| url | https://www.mdpi.com/2076-2607/10/1/101 |
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