Comparative Study on the Sand Bioconsolidation through Calcium Carbonate Precipitation by Sporosarcina pasteurii and Bacillus subtilis
To investigate potential implications of microbial activity on sand bioconsolidation and subsurface environments, two ureolytic strains, Sporosarcina pasteurii and Bacillus subtilis were tested for the production of calcium carbonate (CaCO3). Laboratory experiments with monoculture S. pasteurii (col...
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MDPI AG
2018-04-01
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| Series: | Crystals |
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| Online Access: | http://www.mdpi.com/2073-4352/8/5/189 |
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| author | Chun-Mei Hsu Yi-Hsun Huang Vanita Roshan Nimje Wen-Chien Lee How-Ji Chen Yi-Hao Kuo Chung-Ho Huang Chien-Cheng Chen Chien-Yen Chen |
| author_facet | Chun-Mei Hsu Yi-Hsun Huang Vanita Roshan Nimje Wen-Chien Lee How-Ji Chen Yi-Hao Kuo Chung-Ho Huang Chien-Cheng Chen Chien-Yen Chen |
| author_sort | Chun-Mei Hsu |
| collection | DOAJ |
| description | To investigate potential implications of microbial activity on sand bioconsolidation and subsurface environments, two ureolytic strains, Sporosarcina pasteurii and Bacillus subtilis were tested for the production of calcium carbonate (CaCO3). Laboratory experiments with monoculture S. pasteurii (column 1) and coculture S. pasteurii-B. subtilis (column 2) were conducted to determine urea and calcium chloride reactivity and volumetric carbonate formation. Both columns were able to consolidate sand, whereas, column 1 induced greater CaCO3 precipitation. X-ray diffraction (XRD) and scanning electron microscopy (SEM) showed two columns with different mineralogy with calcite, and vaterite formation. Column 1 showed rhombohedral and trigonal crystals morphology, whereas column 2 developed the prismatic calcite and the spherulite vaterite crystals might be due to the differences of the micro-environment caused by the urease expression of these bacterial species. These results indicate the possibility of using those crystals to cement loose sand whereas, highlighted the importance of combining these techniques to understand the geomicrobiology found in the subsurface environments. |
| first_indexed | 2024-04-11T12:08:23Z |
| format | Article |
| id | doaj.art-571daac2ce30421f93143e77c2455c33 |
| institution | Directory Open Access Journal |
| issn | 2073-4352 |
| language | English |
| last_indexed | 2024-04-11T12:08:23Z |
| publishDate | 2018-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Crystals |
| spelling | doaj.art-571daac2ce30421f93143e77c2455c332022-12-22T04:24:40ZengMDPI AGCrystals2073-43522018-04-018518910.3390/cryst8050189cryst8050189Comparative Study on the Sand Bioconsolidation through Calcium Carbonate Precipitation by Sporosarcina pasteurii and Bacillus subtilisChun-Mei Hsu0Yi-Hsun Huang1Vanita Roshan Nimje2Wen-Chien Lee3How-Ji Chen4Yi-Hao Kuo5Chung-Ho Huang6Chien-Cheng Chen7Chien-Yen Chen8Department of Chemical Engineering, National Chung Cheng University, Chiayi 62102, TaiwanDepartment of Civil Engineering, National Chung-Hsing University, Taichung 40227, TaiwanDepartment of Biomedical Sciences, National Chung Cheng University, Chiayi 62102, TaiwanDepartment of Chemical Engineering, National Chung Cheng University, Chiayi 62102, TaiwanDepartment of Civil Engineering, National Chung-Hsing University, Taichung 40227, TaiwanDepartment of Civil Engineering, National Chung-Hsing University, Taichung 40227, TaiwanDepartment of Civil Engineering, National Taipei University of Technology, Taipei 10655, TaiwanDepartment of Biotechnology, National Kaohsiung Normal University, Kaohsiung 82444, TaiwanDepartment of Earth and Environmental Sciences, National Chung Cheng University, Chiayi 62102, TaiwanTo investigate potential implications of microbial activity on sand bioconsolidation and subsurface environments, two ureolytic strains, Sporosarcina pasteurii and Bacillus subtilis were tested for the production of calcium carbonate (CaCO3). Laboratory experiments with monoculture S. pasteurii (column 1) and coculture S. pasteurii-B. subtilis (column 2) were conducted to determine urea and calcium chloride reactivity and volumetric carbonate formation. Both columns were able to consolidate sand, whereas, column 1 induced greater CaCO3 precipitation. X-ray diffraction (XRD) and scanning electron microscopy (SEM) showed two columns with different mineralogy with calcite, and vaterite formation. Column 1 showed rhombohedral and trigonal crystals morphology, whereas column 2 developed the prismatic calcite and the spherulite vaterite crystals might be due to the differences of the micro-environment caused by the urease expression of these bacterial species. These results indicate the possibility of using those crystals to cement loose sand whereas, highlighted the importance of combining these techniques to understand the geomicrobiology found in the subsurface environments.http://www.mdpi.com/2073-4352/8/5/189MICPSporosarcina pasteuriiBacillus subtilisbacterial extracellular secretionurease |
| spellingShingle | Chun-Mei Hsu Yi-Hsun Huang Vanita Roshan Nimje Wen-Chien Lee How-Ji Chen Yi-Hao Kuo Chung-Ho Huang Chien-Cheng Chen Chien-Yen Chen Comparative Study on the Sand Bioconsolidation through Calcium Carbonate Precipitation by Sporosarcina pasteurii and Bacillus subtilis Crystals MICP Sporosarcina pasteurii Bacillus subtilis bacterial extracellular secretion urease |
| title | Comparative Study on the Sand Bioconsolidation through Calcium Carbonate Precipitation by Sporosarcina pasteurii and Bacillus subtilis |
| title_full | Comparative Study on the Sand Bioconsolidation through Calcium Carbonate Precipitation by Sporosarcina pasteurii and Bacillus subtilis |
| title_fullStr | Comparative Study on the Sand Bioconsolidation through Calcium Carbonate Precipitation by Sporosarcina pasteurii and Bacillus subtilis |
| title_full_unstemmed | Comparative Study on the Sand Bioconsolidation through Calcium Carbonate Precipitation by Sporosarcina pasteurii and Bacillus subtilis |
| title_short | Comparative Study on the Sand Bioconsolidation through Calcium Carbonate Precipitation by Sporosarcina pasteurii and Bacillus subtilis |
| title_sort | comparative study on the sand bioconsolidation through calcium carbonate precipitation by sporosarcina pasteurii and bacillus subtilis |
| topic | MICP Sporosarcina pasteurii Bacillus subtilis bacterial extracellular secretion urease |
| url | http://www.mdpi.com/2073-4352/8/5/189 |
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