Cell wall disruption, membrane damage, and decrease in the expression of Yrp1 virulence factor in Yersinia ruckeri by propolis ethanol extract
Background and Objectives: Instead of antibiotics, propolis is a promising alternative for treating bacterial diseases. The aim of this study was to evaluate the effect of propolis ethanol extract (PEE) on Yersinia ruckeri (Y. ruckeri), a fish pathogen, by examining its impact on the cell wall, cyt...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2023-08-01
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| Series: | Iranian Journal of Microbiology |
| Subjects: | |
| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/4179 |
| _version_ | 1797688891942109184 |
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| author | Fardin Sheydai Amir Tukmechi |
| author_facet | Fardin Sheydai Amir Tukmechi |
| author_sort | Fardin Sheydai |
| collection | DOAJ |
| description |
Background and Objectives: Instead of antibiotics, propolis is a promising alternative for treating bacterial diseases. The aim of this study was to evaluate the effect of propolis ethanol extract (PEE) on Yersinia ruckeri (Y. ruckeri), a fish pathogen, by examining its impact on the cell wall, cytoplasmic membrane, and gene expression.
Materials and Methods: The effect of propolis on the bacterial cell wall, membrane, and DNA using scanning electron microscopy (SEM) was investigated. Its effect on the NAD+/NADH ratio, reactive oxygen species (ROS) production, as well as the expression of a virulence factor (yrp1) was also determined.
Results: It was demonstrated that PEE has multiple antibacterial mechanisms against Y. ruckeri involving cell wall damage, membrane lysis, and a decrease in gene expression.
Conclusion: The obtained results indicated that the mode of propolis action against Y. ruckeri is both structural and functional, while others showed propolis only could inactivate bacteria in a structural way.
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| first_indexed | 2024-03-12T01:37:07Z |
| format | Article |
| id | doaj.art-571e6ab7ce3e4c25b2addd3a90b33bd0 |
| institution | Directory Open Access Journal |
| issn | 2008-3289 2008-4447 |
| language | English |
| last_indexed | 2024-03-12T01:37:07Z |
| publishDate | 2023-08-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj.art-571e6ab7ce3e4c25b2addd3a90b33bd02023-09-11T05:04:04ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472023-08-0115410.18502/ijm.v15i4.13507Cell wall disruption, membrane damage, and decrease in the expression of Yrp1 virulence factor in Yersinia ruckeri by propolis ethanol extractFardin Sheydai0Amir Tukmechi1Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, IranDepartment of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran Background and Objectives: Instead of antibiotics, propolis is a promising alternative for treating bacterial diseases. The aim of this study was to evaluate the effect of propolis ethanol extract (PEE) on Yersinia ruckeri (Y. ruckeri), a fish pathogen, by examining its impact on the cell wall, cytoplasmic membrane, and gene expression. Materials and Methods: The effect of propolis on the bacterial cell wall, membrane, and DNA using scanning electron microscopy (SEM) was investigated. Its effect on the NAD+/NADH ratio, reactive oxygen species (ROS) production, as well as the expression of a virulence factor (yrp1) was also determined. Results: It was demonstrated that PEE has multiple antibacterial mechanisms against Y. ruckeri involving cell wall damage, membrane lysis, and a decrease in gene expression. Conclusion: The obtained results indicated that the mode of propolis action against Y. ruckeri is both structural and functional, while others showed propolis only could inactivate bacteria in a structural way. https://ijm.tums.ac.ir/index.php/ijm/article/view/4179Animals;Yersinia ruckeri;Propolis;Virulence factor;Anti-bacterial agents;Gene expression |
| spellingShingle | Fardin Sheydai Amir Tukmechi Cell wall disruption, membrane damage, and decrease in the expression of Yrp1 virulence factor in Yersinia ruckeri by propolis ethanol extract Iranian Journal of Microbiology Animals; Yersinia ruckeri; Propolis; Virulence factor; Anti-bacterial agents; Gene expression |
| title | Cell wall disruption, membrane damage, and decrease in the expression of Yrp1 virulence factor in Yersinia ruckeri by propolis ethanol extract |
| title_full | Cell wall disruption, membrane damage, and decrease in the expression of Yrp1 virulence factor in Yersinia ruckeri by propolis ethanol extract |
| title_fullStr | Cell wall disruption, membrane damage, and decrease in the expression of Yrp1 virulence factor in Yersinia ruckeri by propolis ethanol extract |
| title_full_unstemmed | Cell wall disruption, membrane damage, and decrease in the expression of Yrp1 virulence factor in Yersinia ruckeri by propolis ethanol extract |
| title_short | Cell wall disruption, membrane damage, and decrease in the expression of Yrp1 virulence factor in Yersinia ruckeri by propolis ethanol extract |
| title_sort | cell wall disruption membrane damage and decrease in the expression of yrp1 virulence factor in yersinia ruckeri by propolis ethanol extract |
| topic | Animals; Yersinia ruckeri; Propolis; Virulence factor; Anti-bacterial agents; Gene expression |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/4179 |
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