Determination of Asymptomatic Chlamydia Trachomatis Infections by Omp1 Gene Based -PCR
Objective: The objective of this research was to determine the prevalenceof genital C. trachomatis infection in asymptomatic women by using highlysensitive nested-polymerase chain reaction (PCR) in urine sample.Materials and Methods: One hundred-forty asymptomatic women wererandomly selected from th...
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| Format: | Article |
| Language: | English |
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Royan Institute (ACECR), Tehran
2008-01-01
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| Series: | Cell Journal |
| Subjects: | |
| Online Access: | http://celljournal.org/library/upload/article/Nazer.pdf |
| _version_ | 1828347639203102720 |
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| author | Masoumeh Nazer Jamileh Nowroozi Akbar Mirsalehian Bahram Kazemi Tahereh Mousavi Abolfazl Mehdizadeh |
| author_facet | Masoumeh Nazer Jamileh Nowroozi Akbar Mirsalehian Bahram Kazemi Tahereh Mousavi Abolfazl Mehdizadeh |
| author_sort | Masoumeh Nazer |
| collection | DOAJ |
| description | Objective: The objective of this research was to determine the prevalenceof genital C. trachomatis infection in asymptomatic women by using highlysensitive nested-polymerase chain reaction (PCR) in urine sample.Materials and Methods: One hundred-forty asymptomatic women wererandomly selected from those who attended gynecology out patient departmentof Hazraat e Rasool Hospital in Tehran. First catch urine specimen were collectedfrom all the participants. DNA extraction was performed by means of HighPure PCR Template Preparation Kit (HPPTP) according to the manufacture’sinstructions. Extracted DNA was tested by omp1 gene based nested-PCR,using sets of primers to amplify C. trachomatis omp1 gene. Visualizationof a 1027 bp fragment from omp1 gene in agarose gel electrophoresis wasconsidered as a positive result.Results: In total, 140 urines were tested for determination of C. trachomatisinfection. C. trachomatis omp-1 was detected in 22.1% of cases (31/140). Theoverall prevalence rates of C. trachomatis in the urine sample as determined byomp1 based nested-PCR were 4.3% in group I (age, <25 years), 12.1% in groupII (age, 25-34 years), 5.0% in group III (age, 35-44 years) and 0.7% in group IV(>44 years). The highest prevalence of C. trachomatis infection (12.1%) wasseen in women aged 25-34 years. This finding was not statistically significant(p=0.710). Also, there was not relation between C. trachomatis infection andsome probable risk factors such as young age (<25 years), STD history andmissing use of barrier contraceptive in this study.Conclusion: The prevalence of C.trachomatis infection in the women notseeking health care warrants more comprehensive study using high sensitiveomp1 based nested- PCR to identify and treat a large number of infectedwomen in Iran. |
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| format | Article |
| id | doaj.art-571f63f9b34f4cd99daaa22d4cfc1197 |
| institution | Directory Open Access Journal |
| issn | 2228-5806 2228-5814 |
| language | English |
| last_indexed | 2024-04-14T00:42:17Z |
| publishDate | 2008-01-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | Cell Journal |
| spelling | doaj.art-571f63f9b34f4cd99daaa22d4cfc11972022-12-22T02:22:08ZengRoyan Institute (ACECR), TehranCell Journal2228-58062228-58142008-01-011014146Determination of Asymptomatic Chlamydia Trachomatis Infections by Omp1 Gene Based -PCRMasoumeh NazerJamileh NowrooziAkbar MirsalehianBahram KazemiTahereh MousaviAbolfazl MehdizadehObjective: The objective of this research was to determine the prevalenceof genital C. trachomatis infection in asymptomatic women by using highlysensitive nested-polymerase chain reaction (PCR) in urine sample.Materials and Methods: One hundred-forty asymptomatic women wererandomly selected from those who attended gynecology out patient departmentof Hazraat e Rasool Hospital in Tehran. First catch urine specimen were collectedfrom all the participants. DNA extraction was performed by means of HighPure PCR Template Preparation Kit (HPPTP) according to the manufacture’sinstructions. Extracted DNA was tested by omp1 gene based nested-PCR,using sets of primers to amplify C. trachomatis omp1 gene. Visualizationof a 1027 bp fragment from omp1 gene in agarose gel electrophoresis wasconsidered as a positive result.Results: In total, 140 urines were tested for determination of C. trachomatisinfection. C. trachomatis omp-1 was detected in 22.1% of cases (31/140). Theoverall prevalence rates of C. trachomatis in the urine sample as determined byomp1 based nested-PCR were 4.3% in group I (age, <25 years), 12.1% in groupII (age, 25-34 years), 5.0% in group III (age, 35-44 years) and 0.7% in group IV(>44 years). The highest prevalence of C. trachomatis infection (12.1%) wasseen in women aged 25-34 years. This finding was not statistically significant(p=0.710). Also, there was not relation between C. trachomatis infection andsome probable risk factors such as young age (<25 years), STD history andmissing use of barrier contraceptive in this study.Conclusion: The prevalence of C.trachomatis infection in the women notseeking health care warrants more comprehensive study using high sensitiveomp1 based nested- PCR to identify and treat a large number of infectedwomen in Iran.http://celljournal.org/library/upload/article/Nazer.pdfChlamydia TrachomatisAsymptomatic Infectionomp1 GeneNested-PCRUrine Sample |
| spellingShingle | Masoumeh Nazer Jamileh Nowroozi Akbar Mirsalehian Bahram Kazemi Tahereh Mousavi Abolfazl Mehdizadeh Determination of Asymptomatic Chlamydia Trachomatis Infections by Omp1 Gene Based -PCR Cell Journal Chlamydia Trachomatis Asymptomatic Infection omp1 Gene Nested-PCR Urine Sample |
| title | Determination of Asymptomatic Chlamydia Trachomatis Infections by Omp1 Gene Based -PCR |
| title_full | Determination of Asymptomatic Chlamydia Trachomatis Infections by Omp1 Gene Based -PCR |
| title_fullStr | Determination of Asymptomatic Chlamydia Trachomatis Infections by Omp1 Gene Based -PCR |
| title_full_unstemmed | Determination of Asymptomatic Chlamydia Trachomatis Infections by Omp1 Gene Based -PCR |
| title_short | Determination of Asymptomatic Chlamydia Trachomatis Infections by Omp1 Gene Based -PCR |
| title_sort | determination of asymptomatic chlamydia trachomatis infections by omp1 gene based pcr |
| topic | Chlamydia Trachomatis Asymptomatic Infection omp1 Gene Nested-PCR Urine Sample |
| url | http://celljournal.org/library/upload/article/Nazer.pdf |
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