Integrated bioinformatics analysis reveals the function and prognostic value of OSBPL3 in hepatocellular carcinoma
Background: Liver hepatocellular carcinoma (LIHC), a variety of highly-aggressive malignancy, has been the major cause of cancer-related mortality. Recent studies have shown that oxysterol-binding protein-like 3 (OSBPL3) plays a crucial role in human cancers. Nevertheless, the specific functional ro...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023044316 |
| _version_ | 1827923821939654656 |
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| author | Yuanshuai Su Chen Xue Xinyu Gu Yu Sun Renfang Zhang Lanjuan Li |
| author_facet | Yuanshuai Su Chen Xue Xinyu Gu Yu Sun Renfang Zhang Lanjuan Li |
| author_sort | Yuanshuai Su |
| collection | DOAJ |
| description | Background: Liver hepatocellular carcinoma (LIHC), a variety of highly-aggressive malignancy, has been the major cause of cancer-related mortality. Recent studies have shown that oxysterol-binding protein-like 3 (OSBPL3) plays a crucial role in human cancers. Nevertheless, the specific functional roles and potential clinical values of OSBPL3 in LIHC are not completely known. Methods: Multiple web portals and publicly available tools were used in this study. Comprehensive expression files of OSBPL3 in pan-cancers and the relationship between OSBPL3 expression and clinical traits of patients with LIHC were investigated using TCGA database through UALCAN platform. TIMER database was used to investigate the effect of OSBPL3 on the tumor immune infiltration status in LIHC. Moreover, LinkedOmics, STRING databases, and Gene Ontology analysis were utilized to select OSBPL3-related differentially expressed genes (DEGs) and construct a protein–protein interaction (PPI) network. Results: Upregulated OSBPL3 was observed in LIHC tumor tissues compared with that in normal controls, especially in patients with higher grades and more advanced stages. Furthermore, overexpressed OSBPL3 was closely associated with poor clinical outcomes of patients with LIHC. Six hub genes were selected from the PPI network, which were significantly increased in LIHC and closely associated with poor prognosis. Pathway enrichment showed that OSBPL3-related DEGs were primarily enriched in protein binding, mitotic cytokinesis, inorganic anion transport, and I-kappaB kinase/NF-kappaB signaling processes. Conclusions: OSBPL3 exerts critical functions in hepatocarcinogenesis and it could serve as an available biomarker and effective treatment target for LIHC. |
| first_indexed | 2024-03-13T05:01:40Z |
| format | Article |
| id | doaj.art-572136f9b9904f5f813ed8905370b356 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-13T05:01:40Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
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| series | Heliyon |
| spelling | doaj.art-572136f9b9904f5f813ed8905370b3562023-06-17T05:19:32ZengElsevierHeliyon2405-84402023-06-0196e17223Integrated bioinformatics analysis reveals the function and prognostic value of OSBPL3 in hepatocellular carcinomaYuanshuai Su0Chen Xue1Xinyu Gu2Yu Sun3Renfang Zhang4Lanjuan Li5State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, National Medical Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, 79 Qingchun Rd., Hangzhou, 310003, ChinaState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, National Medical Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, 79 Qingchun Rd., Hangzhou, 310003, ChinaState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, National Medical Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, 79 Qingchun Rd., Hangzhou, 310003, ChinaState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, National Medical Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, 79 Qingchun Rd., Hangzhou, 310003, ChinaState Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, National Medical Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, 79 Qingchun Rd., Hangzhou, 310003, ChinaCorresponding author.; State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, National Medical Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, 79 Qingchun Rd., Hangzhou, 310003, ChinaBackground: Liver hepatocellular carcinoma (LIHC), a variety of highly-aggressive malignancy, has been the major cause of cancer-related mortality. Recent studies have shown that oxysterol-binding protein-like 3 (OSBPL3) plays a crucial role in human cancers. Nevertheless, the specific functional roles and potential clinical values of OSBPL3 in LIHC are not completely known. Methods: Multiple web portals and publicly available tools were used in this study. Comprehensive expression files of OSBPL3 in pan-cancers and the relationship between OSBPL3 expression and clinical traits of patients with LIHC were investigated using TCGA database through UALCAN platform. TIMER database was used to investigate the effect of OSBPL3 on the tumor immune infiltration status in LIHC. Moreover, LinkedOmics, STRING databases, and Gene Ontology analysis were utilized to select OSBPL3-related differentially expressed genes (DEGs) and construct a protein–protein interaction (PPI) network. Results: Upregulated OSBPL3 was observed in LIHC tumor tissues compared with that in normal controls, especially in patients with higher grades and more advanced stages. Furthermore, overexpressed OSBPL3 was closely associated with poor clinical outcomes of patients with LIHC. Six hub genes were selected from the PPI network, which were significantly increased in LIHC and closely associated with poor prognosis. Pathway enrichment showed that OSBPL3-related DEGs were primarily enriched in protein binding, mitotic cytokinesis, inorganic anion transport, and I-kappaB kinase/NF-kappaB signaling processes. Conclusions: OSBPL3 exerts critical functions in hepatocarcinogenesis and it could serve as an available biomarker and effective treatment target for LIHC.http://www.sciencedirect.com/science/article/pii/S2405844023044316OSBPL3Hepatocellular carcinomaPrognosisImmune infiltrationHub gene |
| spellingShingle | Yuanshuai Su Chen Xue Xinyu Gu Yu Sun Renfang Zhang Lanjuan Li Integrated bioinformatics analysis reveals the function and prognostic value of OSBPL3 in hepatocellular carcinoma Heliyon OSBPL3 Hepatocellular carcinoma Prognosis Immune infiltration Hub gene |
| title | Integrated bioinformatics analysis reveals the function and prognostic value of OSBPL3 in hepatocellular carcinoma |
| title_full | Integrated bioinformatics analysis reveals the function and prognostic value of OSBPL3 in hepatocellular carcinoma |
| title_fullStr | Integrated bioinformatics analysis reveals the function and prognostic value of OSBPL3 in hepatocellular carcinoma |
| title_full_unstemmed | Integrated bioinformatics analysis reveals the function and prognostic value of OSBPL3 in hepatocellular carcinoma |
| title_short | Integrated bioinformatics analysis reveals the function and prognostic value of OSBPL3 in hepatocellular carcinoma |
| title_sort | integrated bioinformatics analysis reveals the function and prognostic value of osbpl3 in hepatocellular carcinoma |
| topic | OSBPL3 Hepatocellular carcinoma Prognosis Immune infiltration Hub gene |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023044316 |
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