Heat Release by Isolated Mouse Brain Mitochondria Detected with Diamond Thermometer
The production of heat by mitochondria is critical for maintaining body temperature, regulating metabolic rate, and preventing oxidative damage to mitochondria and cells. Until the present, mitochondrial heat production has been characterized only by methods based on fluorescent probes, which are se...
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MDPI AG
2022-12-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/13/1/98 |
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| author | Alexey M. Romshin Alexander A. Osypov Irina Yu. Popova Vadim E. Zeeb Andrey G. Sinogeykin Igor I. Vlasov |
| author_facet | Alexey M. Romshin Alexander A. Osypov Irina Yu. Popova Vadim E. Zeeb Andrey G. Sinogeykin Igor I. Vlasov |
| author_sort | Alexey M. Romshin |
| collection | DOAJ |
| description | The production of heat by mitochondria is critical for maintaining body temperature, regulating metabolic rate, and preventing oxidative damage to mitochondria and cells. Until the present, mitochondrial heat production has been characterized only by methods based on fluorescent probes, which are sensitive to environmental variations (viscosity, pH, ionic strength, quenching, etc.). Here, for the first time, the heat release of isolated mitochondria was unambiguously measured by a diamond thermometer (DT), which is absolutely indifferent to external non-thermal parameters. We show that during total uncoupling of transmembrane potential by CCCP application, the temperature near the mitochondria rises by 4–22 °C above the ambient temperature with an absolute maximum of 45 °C. Such a broad variation in the temperature response is associated with the heterogeneity of the mitochondria themselves as well as their aggregations in the isolated suspension. Spontaneous temperature bursts with comparable amplitude were also detected prior to CCCP application, which may reflect involvement of some mitochondria to ATP synthesis or membrane potential leaking to avoid hyperproduction of reactive oxygen species. The results obtained with the diamond temperature sensor shed light on the “hot mitochondria” paradox. |
| first_indexed | 2024-03-09T09:43:34Z |
| format | Article |
| id | doaj.art-5723cb8ea3674f838d69201ee3b30ee3 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-09T09:43:34Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-5723cb8ea3674f838d69201ee3b30ee32023-12-02T00:44:21ZengMDPI AGNanomaterials2079-49912022-12-011319810.3390/nano13010098Heat Release by Isolated Mouse Brain Mitochondria Detected with Diamond ThermometerAlexey M. Romshin0Alexander A. Osypov1Irina Yu. Popova2Vadim E. Zeeb3Andrey G. Sinogeykin4Igor I. Vlasov5Prokhorov General Physics Institute of the Russian Academy of Sciences, 119991 Moscow, RussiaInstitute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences, 142292 Moscow, RussiaInstitute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences, 142292 Moscow, RussiaInstitute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences, 142292 Moscow, RussiaWonder Technologies LLC, 143026 Moscow, RussiaProkhorov General Physics Institute of the Russian Academy of Sciences, 119991 Moscow, RussiaThe production of heat by mitochondria is critical for maintaining body temperature, regulating metabolic rate, and preventing oxidative damage to mitochondria and cells. Until the present, mitochondrial heat production has been characterized only by methods based on fluorescent probes, which are sensitive to environmental variations (viscosity, pH, ionic strength, quenching, etc.). Here, for the first time, the heat release of isolated mitochondria was unambiguously measured by a diamond thermometer (DT), which is absolutely indifferent to external non-thermal parameters. We show that during total uncoupling of transmembrane potential by CCCP application, the temperature near the mitochondria rises by 4–22 °C above the ambient temperature with an absolute maximum of 45 °C. Such a broad variation in the temperature response is associated with the heterogeneity of the mitochondria themselves as well as their aggregations in the isolated suspension. Spontaneous temperature bursts with comparable amplitude were also detected prior to CCCP application, which may reflect involvement of some mitochondria to ATP synthesis or membrane potential leaking to avoid hyperproduction of reactive oxygen species. The results obtained with the diamond temperature sensor shed light on the “hot mitochondria” paradox.https://www.mdpi.com/2079-4991/13/1/98diamond thermometermitochondriacell thermodynamics |
| spellingShingle | Alexey M. Romshin Alexander A. Osypov Irina Yu. Popova Vadim E. Zeeb Andrey G. Sinogeykin Igor I. Vlasov Heat Release by Isolated Mouse Brain Mitochondria Detected with Diamond Thermometer Nanomaterials diamond thermometer mitochondria cell thermodynamics |
| title | Heat Release by Isolated Mouse Brain Mitochondria Detected with Diamond Thermometer |
| title_full | Heat Release by Isolated Mouse Brain Mitochondria Detected with Diamond Thermometer |
| title_fullStr | Heat Release by Isolated Mouse Brain Mitochondria Detected with Diamond Thermometer |
| title_full_unstemmed | Heat Release by Isolated Mouse Brain Mitochondria Detected with Diamond Thermometer |
| title_short | Heat Release by Isolated Mouse Brain Mitochondria Detected with Diamond Thermometer |
| title_sort | heat release by isolated mouse brain mitochondria detected with diamond thermometer |
| topic | diamond thermometer mitochondria cell thermodynamics |
| url | https://www.mdpi.com/2079-4991/13/1/98 |
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