Optically gated beating-heart imaging

The constant motion of the beating heart presents an obstacle to clear optical imaging, especially 3D imaging, in small animals where direct optical imaging would otherwise be possible. Gating techniques exploit the periodic motion of the heart to computationally freeze this movement and overcome mo...

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Main Author: Jonathan M Taylor
Format: Article
Language:English
Published: Frontiers Media S.A. 2014-12-01
Series:Frontiers in Physiology
Subjects:
Online Access:http://journal.frontiersin.org/Journal/10.3389/fphys.2014.00481/full
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author Jonathan M Taylor
author_facet Jonathan M Taylor
author_sort Jonathan M Taylor
collection DOAJ
description The constant motion of the beating heart presents an obstacle to clear optical imaging, especially 3D imaging, in small animals where direct optical imaging would otherwise be possible. Gating techniques exploit the periodic motion of the heart to computationally freeze this movement and overcome motion artefacts. Optically gated imaging represents a recent development of this, where image analysis is used to synchronize acquisition with the heartbeat in a completely non-invasive manner. This article will explain the concept of optical gating, discuss a range of different implementation strategies and their strengths and weaknesses. Finally we will illustrate the usefulness of the technique by discussing applications where optical gating has facilitated novel biological findings by allowing 3D in vivo imaging of cardiac myocytes in their natural environment of the beating heart.
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spelling doaj.art-57250941b1ce40429274f414f8cc78382022-12-22T01:46:52ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2014-12-01510.3389/fphys.2014.00481112309Optically gated beating-heart imagingJonathan M Taylor0Glasgow UniversityThe constant motion of the beating heart presents an obstacle to clear optical imaging, especially 3D imaging, in small animals where direct optical imaging would otherwise be possible. Gating techniques exploit the periodic motion of the heart to computationally freeze this movement and overcome motion artefacts. Optically gated imaging represents a recent development of this, where image analysis is used to synchronize acquisition with the heartbeat in a completely non-invasive manner. This article will explain the concept of optical gating, discuss a range of different implementation strategies and their strengths and weaknesses. Finally we will illustrate the usefulness of the technique by discussing applications where optical gating has facilitated novel biological findings by allowing 3D in vivo imaging of cardiac myocytes in their natural environment of the beating heart.http://journal.frontiersin.org/Journal/10.3389/fphys.2014.00481/full3D imagingoptical gatingheart imagingcardiac imagingheart synchronizationmotion compensation
spellingShingle Jonathan M Taylor
Optically gated beating-heart imaging
Frontiers in Physiology
3D imaging
optical gating
heart imaging
cardiac imaging
heart synchronization
motion compensation
title Optically gated beating-heart imaging
title_full Optically gated beating-heart imaging
title_fullStr Optically gated beating-heart imaging
title_full_unstemmed Optically gated beating-heart imaging
title_short Optically gated beating-heart imaging
title_sort optically gated beating heart imaging
topic 3D imaging
optical gating
heart imaging
cardiac imaging
heart synchronization
motion compensation
url http://journal.frontiersin.org/Journal/10.3389/fphys.2014.00481/full
work_keys_str_mv AT jonathanmtaylor opticallygatedbeatingheartimaging