Optically gated beating-heart imaging
The constant motion of the beating heart presents an obstacle to clear optical imaging, especially 3D imaging, in small animals where direct optical imaging would otherwise be possible. Gating techniques exploit the periodic motion of the heart to computationally freeze this movement and overcome mo...
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Format: | Article |
Language: | English |
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Frontiers Media S.A.
2014-12-01
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Series: | Frontiers in Physiology |
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Online Access: | http://journal.frontiersin.org/Journal/10.3389/fphys.2014.00481/full |
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author | Jonathan M Taylor |
author_facet | Jonathan M Taylor |
author_sort | Jonathan M Taylor |
collection | DOAJ |
description | The constant motion of the beating heart presents an obstacle to clear optical imaging, especially 3D imaging, in small animals where direct optical imaging would otherwise be possible. Gating techniques exploit the periodic motion of the heart to computationally freeze this movement and overcome motion artefacts. Optically gated imaging represents a recent development of this, where image analysis is used to synchronize acquisition with the heartbeat in a completely non-invasive manner. This article will explain the concept of optical gating, discuss a range of different implementation strategies and their strengths and weaknesses. Finally we will illustrate the usefulness of the technique by discussing applications where optical gating has facilitated novel biological findings by allowing 3D in vivo imaging of cardiac myocytes in their natural environment of the beating heart. |
first_indexed | 2024-12-10T13:34:42Z |
format | Article |
id | doaj.art-57250941b1ce40429274f414f8cc7838 |
institution | Directory Open Access Journal |
issn | 1664-042X |
language | English |
last_indexed | 2024-12-10T13:34:42Z |
publishDate | 2014-12-01 |
publisher | Frontiers Media S.A. |
record_format | Article |
series | Frontiers in Physiology |
spelling | doaj.art-57250941b1ce40429274f414f8cc78382022-12-22T01:46:52ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2014-12-01510.3389/fphys.2014.00481112309Optically gated beating-heart imagingJonathan M Taylor0Glasgow UniversityThe constant motion of the beating heart presents an obstacle to clear optical imaging, especially 3D imaging, in small animals where direct optical imaging would otherwise be possible. Gating techniques exploit the periodic motion of the heart to computationally freeze this movement and overcome motion artefacts. Optically gated imaging represents a recent development of this, where image analysis is used to synchronize acquisition with the heartbeat in a completely non-invasive manner. This article will explain the concept of optical gating, discuss a range of different implementation strategies and their strengths and weaknesses. Finally we will illustrate the usefulness of the technique by discussing applications where optical gating has facilitated novel biological findings by allowing 3D in vivo imaging of cardiac myocytes in their natural environment of the beating heart.http://journal.frontiersin.org/Journal/10.3389/fphys.2014.00481/full3D imagingoptical gatingheart imagingcardiac imagingheart synchronizationmotion compensation |
spellingShingle | Jonathan M Taylor Optically gated beating-heart imaging Frontiers in Physiology 3D imaging optical gating heart imaging cardiac imaging heart synchronization motion compensation |
title | Optically gated beating-heart imaging |
title_full | Optically gated beating-heart imaging |
title_fullStr | Optically gated beating-heart imaging |
title_full_unstemmed | Optically gated beating-heart imaging |
title_short | Optically gated beating-heart imaging |
title_sort | optically gated beating heart imaging |
topic | 3D imaging optical gating heart imaging cardiac imaging heart synchronization motion compensation |
url | http://journal.frontiersin.org/Journal/10.3389/fphys.2014.00481/full |
work_keys_str_mv | AT jonathanmtaylor opticallygatedbeatingheartimaging |