Wheat straw lignin degradation induction to aromatics by por Aspergillus spp. and Penicillium chrysogenum
Wheat straw is a recalcitrant agricultural waste; incineration of this material represents an important environmental impact. Different reports have been made regarding the use of the structural components of wheat straw, i.e. cellulose, hemicellulose and lignin; however, lignin has been less exploi...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | Spanish |
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Selva Andina Research Society
2016-02-01
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| Series: | Journal of the Selva Andina Research Society |
| Online Access: | http://www.scielo.org.bo/scielo.php?script=sci_arttext&pid=S2072-92942016000100003&lng=es&nrm=iso&tlng=es |
| _version_ | 1818017907999768576 |
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| author | Baltierra-Trejo Eduardo Silva-Espino Eliseo Márquez-Benavides Liliana Sánchez-Yáñez Juan Manuel |
| author_facet | Baltierra-Trejo Eduardo Silva-Espino Eliseo Márquez-Benavides Liliana Sánchez-Yáñez Juan Manuel |
| author_sort | Baltierra-Trejo Eduardo |
| collection | DOAJ |
| description | Wheat straw is a recalcitrant agricultural waste; incineration of this material represents an important environmental impact. Different reports have been made regarding the use of the structural components of wheat straw, i.e. cellulose, hemicellulose and lignin; however, lignin has been less exploited because it is largely considered the recalcitrant part. Residual wheat straw lignin (REWSLI) has a potential biotech-nological value if depolymerization is attained to produce aromatics. Ligninolytic mitosporic fungus represent an alternative where very little research has been done, even though they are capable of depol-ymerize REWSLI in simple nutritional conditions in relatively short periods, when compared to basidio-mycetes. The aim of this research was to study the depolymerization activity of Aspergillus spp and Penicillium spp on semipurified REWSLI as the sole carbon source to produce aromatics. The depoly-merization capacity was determined by the activity of the laccase, lignin peroxidase and manganese peroxidase enzymes. The generated aromatics derived from the REWSLI depolymerization were identi-fied by gas chromatography. Obtained results revealed that Penicillium chrysogenum depolymerized the lignin material by 34.8% during the 28-day experimentation period. Laccase activity showed the largest activity with 111 U L-1 in a seven-day period, this enzyme induction was detected in a smaller period than that required by basidiomycetes to induce it. Moreover, the enzymatic activity was produced with-out the addition of an extra carbon source as metabolic inductor. Aspergillus spp and Penicillium spp generated guaiacol, vanillin, and hydroxybenzoic, vanillinic, syringic and ferulic acid with a maximum weekly production of 3.5, 3.3, 3.2, 3.3, 10.1 and 21.9 mg mL-1, respectively. |
| first_indexed | 2024-04-14T07:32:51Z |
| format | Article |
| id | doaj.art-57254c11052a42b0bd0ae2e5cf4ee737 |
| institution | Directory Open Access Journal |
| issn | 2072-9294 2072-9308 |
| language | Spanish |
| last_indexed | 2024-04-14T07:32:51Z |
| publishDate | 2016-02-01 |
| publisher | Selva Andina Research Society |
| record_format | Article |
| series | Journal of the Selva Andina Research Society |
| spelling | doaj.art-57254c11052a42b0bd0ae2e5cf4ee7372022-12-22T02:05:48ZspaSelva Andina Research SocietyJournal of the Selva Andina Research Society2072-92942072-93082016-02-01711019Wheat straw lignin degradation induction to aromatics by por Aspergillus spp. and Penicillium chrysogenumBaltierra-Trejo EduardoSilva-Espino EliseoMárquez-Benavides LilianaSánchez-Yáñez Juan ManuelWheat straw is a recalcitrant agricultural waste; incineration of this material represents an important environmental impact. Different reports have been made regarding the use of the structural components of wheat straw, i.e. cellulose, hemicellulose and lignin; however, lignin has been less exploited because it is largely considered the recalcitrant part. Residual wheat straw lignin (REWSLI) has a potential biotech-nological value if depolymerization is attained to produce aromatics. Ligninolytic mitosporic fungus represent an alternative where very little research has been done, even though they are capable of depol-ymerize REWSLI in simple nutritional conditions in relatively short periods, when compared to basidio-mycetes. The aim of this research was to study the depolymerization activity of Aspergillus spp and Penicillium spp on semipurified REWSLI as the sole carbon source to produce aromatics. The depoly-merization capacity was determined by the activity of the laccase, lignin peroxidase and manganese peroxidase enzymes. The generated aromatics derived from the REWSLI depolymerization were identi-fied by gas chromatography. Obtained results revealed that Penicillium chrysogenum depolymerized the lignin material by 34.8% during the 28-day experimentation period. Laccase activity showed the largest activity with 111 U L-1 in a seven-day period, this enzyme induction was detected in a smaller period than that required by basidiomycetes to induce it. Moreover, the enzymatic activity was produced with-out the addition of an extra carbon source as metabolic inductor. Aspergillus spp and Penicillium spp generated guaiacol, vanillin, and hydroxybenzoic, vanillinic, syringic and ferulic acid with a maximum weekly production of 3.5, 3.3, 3.2, 3.3, 10.1 and 21.9 mg mL-1, respectively.http://www.scielo.org.bo/scielo.php?script=sci_arttext&pid=S2072-92942016000100003&lng=es&nrm=iso&tlng=es |
| spellingShingle | Baltierra-Trejo Eduardo Silva-Espino Eliseo Márquez-Benavides Liliana Sánchez-Yáñez Juan Manuel Wheat straw lignin degradation induction to aromatics by por Aspergillus spp. and Penicillium chrysogenum Journal of the Selva Andina Research Society |
| title | Wheat straw lignin degradation induction to aromatics by por Aspergillus spp. and Penicillium chrysogenum |
| title_full | Wheat straw lignin degradation induction to aromatics by por Aspergillus spp. and Penicillium chrysogenum |
| title_fullStr | Wheat straw lignin degradation induction to aromatics by por Aspergillus spp. and Penicillium chrysogenum |
| title_full_unstemmed | Wheat straw lignin degradation induction to aromatics by por Aspergillus spp. and Penicillium chrysogenum |
| title_short | Wheat straw lignin degradation induction to aromatics by por Aspergillus spp. and Penicillium chrysogenum |
| title_sort | wheat straw lignin degradation induction to aromatics by por aspergillus spp and penicillium chrysogenum |
| url | http://www.scielo.org.bo/scielo.php?script=sci_arttext&pid=S2072-92942016000100003&lng=es&nrm=iso&tlng=es |
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