Comparison of the structure and immunomodulatory activity of polysaccharides from fresh and dried longan
The structures and immunomodulatory activities of purified neutral polysaccharides from fresh (FLP) and hot air-dried (DLP) longan were comparatively investigated. FLP was composed of mannose and glucose at a molar ratio of 0.59:1 with average molecular weight of 1.31 × 107 g/mol, and DLP was compos...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-01-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464620305478 |
| _version_ | 1818728276565164032 |
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| author | Tingsheng Gan Chao Feng Haibo Lan Ruili Yang Jiachao Zhang Congfa Li Wu Li |
| author_facet | Tingsheng Gan Chao Feng Haibo Lan Ruili Yang Jiachao Zhang Congfa Li Wu Li |
| author_sort | Tingsheng Gan |
| collection | DOAJ |
| description | The structures and immunomodulatory activities of purified neutral polysaccharides from fresh (FLP) and hot air-dried (DLP) longan were comparatively investigated. FLP was composed of mannose and glucose at a molar ratio of 0.59:1 with average molecular weight of 1.31 × 107 g/mol, and DLP was composed of rhamnose, mannose, glucose and galactose at a molar ratio of 0.2:0.21:1:0.2 with average molecular weight of 1.06 × 107 g/mol. Methylation and NMR data showed that FLP was primarily composed of →6)-α-D-Glcp-(1→, and that DLP was primarily composed of →6)-α-D-Glcp-(1→, →3)-β-D-Glcp-(1→, →3)-β-D-Galp-(1→ and →3)-α-L-Rhap-(1→. FLP and DLP significantly enhanced lymphocyte proliferation, phagocytosis and NO, IL-1β and IL-6 production by macrophages. The drying process increased degree of branching and significantly affected the immunomodulatory activity of longan polysaccharides. DLP exhibited a strong effect on stimulation of the proliferation of lymphocytes and the activation of macrophages. The knowledge of effects of drying process on the structures and bioactivity of longan polysaccharides can aid in illuminating the basis for application of dried longan in the functional foods and medicines production. |
| first_indexed | 2024-12-17T22:27:25Z |
| format | Article |
| id | doaj.art-572562a61bc64aadb53389d6605b476b |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-17T22:27:25Z |
| publishDate | 2021-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-572562a61bc64aadb53389d6605b476b2022-12-21T21:30:18ZengElsevierJournal of Functional Foods1756-46462021-01-0176104323Comparison of the structure and immunomodulatory activity of polysaccharides from fresh and dried longanTingsheng Gan0Chao Feng1Haibo Lan2Ruili Yang3Jiachao Zhang4Congfa Li5Wu Li6Key Laboratory of Food Nutrition and Functional Food of Hainan Province, College of Food Science and Engineering, Hainan University, Haikou 570228, ChinaKey Laboratory of Food Nutrition and Functional Food of Hainan Province, College of Food Science and Engineering, Hainan University, Haikou 570228, ChinaKey Laboratory of Food Nutrition and Functional Food of Hainan Province, College of Food Science and Engineering, Hainan University, Haikou 570228, China; College of Food Science, South China Agricultural University, Guangzhou 510642, ChinaCollege of Food Science, South China Agricultural University, Guangzhou 510642, ChinaKey Laboratory of Food Nutrition and Functional Food of Hainan Province, College of Food Science and Engineering, Hainan University, Haikou 570228, China; Corresponding authors.Key Laboratory of Food Nutrition and Functional Food of Hainan Province, College of Food Science and Engineering, Hainan University, Haikou 570228, ChinaKey Laboratory of Food Nutrition and Functional Food of Hainan Province, College of Food Science and Engineering, Hainan University, Haikou 570228, China; Corresponding authors.The structures and immunomodulatory activities of purified neutral polysaccharides from fresh (FLP) and hot air-dried (DLP) longan were comparatively investigated. FLP was composed of mannose and glucose at a molar ratio of 0.59:1 with average molecular weight of 1.31 × 107 g/mol, and DLP was composed of rhamnose, mannose, glucose and galactose at a molar ratio of 0.2:0.21:1:0.2 with average molecular weight of 1.06 × 107 g/mol. Methylation and NMR data showed that FLP was primarily composed of →6)-α-D-Glcp-(1→, and that DLP was primarily composed of →6)-α-D-Glcp-(1→, →3)-β-D-Glcp-(1→, →3)-β-D-Galp-(1→ and →3)-α-L-Rhap-(1→. FLP and DLP significantly enhanced lymphocyte proliferation, phagocytosis and NO, IL-1β and IL-6 production by macrophages. The drying process increased degree of branching and significantly affected the immunomodulatory activity of longan polysaccharides. DLP exhibited a strong effect on stimulation of the proliferation of lymphocytes and the activation of macrophages. The knowledge of effects of drying process on the structures and bioactivity of longan polysaccharides can aid in illuminating the basis for application of dried longan in the functional foods and medicines production.http://www.sciencedirect.com/science/article/pii/S1756464620305478DryingLongan polysaccharidesStructural featuresImmunomodulatory activities |
| spellingShingle | Tingsheng Gan Chao Feng Haibo Lan Ruili Yang Jiachao Zhang Congfa Li Wu Li Comparison of the structure and immunomodulatory activity of polysaccharides from fresh and dried longan Journal of Functional Foods Drying Longan polysaccharides Structural features Immunomodulatory activities |
| title | Comparison of the structure and immunomodulatory activity of polysaccharides from fresh and dried longan |
| title_full | Comparison of the structure and immunomodulatory activity of polysaccharides from fresh and dried longan |
| title_fullStr | Comparison of the structure and immunomodulatory activity of polysaccharides from fresh and dried longan |
| title_full_unstemmed | Comparison of the structure and immunomodulatory activity of polysaccharides from fresh and dried longan |
| title_short | Comparison of the structure and immunomodulatory activity of polysaccharides from fresh and dried longan |
| title_sort | comparison of the structure and immunomodulatory activity of polysaccharides from fresh and dried longan |
| topic | Drying Longan polysaccharides Structural features Immunomodulatory activities |
| url | http://www.sciencedirect.com/science/article/pii/S1756464620305478 |
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