A Methylene Blue Assisted Electrochemical Sensor for Determination of Drug Resistance of Escherichia coli
Due to the abuse of antibiotics in clinical, animal husbandry, and aquaculture, drug-resistant pathogens are produced, which poses a great threat to human and the public health. At present, a rapid and effective drug sensitivity test method is urgently needed to effectively control the spread of dru...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-05-01
|
| Series: | Frontiers in Chemistry |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fchem.2021.689735/full |
| _version_ | 1818833090006482944 |
|---|---|
| author | Rongshuai Duan Rongshuai Duan Xiao Fang Dongliang Wang Dongliang Wang |
| author_facet | Rongshuai Duan Rongshuai Duan Xiao Fang Dongliang Wang Dongliang Wang |
| author_sort | Rongshuai Duan |
| collection | DOAJ |
| description | Due to the abuse of antibiotics in clinical, animal husbandry, and aquaculture, drug-resistant pathogens are produced, which poses a great threat to human and the public health. At present, a rapid and effective drug sensitivity test method is urgently needed to effectively control the spread of drug-resistant bacteria. Using methylene blue as a redox probe, the electrochemical signals of methylene blue in drug-resistant Escherichia coli strains were analyzed by a CV method. Graphene ink has been used for enhancing the electrochemical signal. Compared with the results of the traditional drug sensitivity test, we proposed a rapid electrochemical drug sensitivity test method which can effectively identify the drug sensitivity of Escherichia coli. The sensitivity of four E. coli isolates to ciprofloxacin, gentamicin, and ampicillin was tested by an electrochemical drug sensitivity test. The respiratory activity value %RA was used as an indicator of bacterial resistance by electrochemical method. |
| first_indexed | 2024-12-19T02:13:23Z |
| format | Article |
| id | doaj.art-5728948a59e14a4ab0f3351f6409c4c7 |
| institution | Directory Open Access Journal |
| issn | 2296-2646 |
| language | English |
| last_indexed | 2024-12-19T02:13:23Z |
| publishDate | 2021-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Chemistry |
| spelling | doaj.art-5728948a59e14a4ab0f3351f6409c4c72022-12-21T20:40:33ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462021-05-01910.3389/fchem.2021.689735689735A Methylene Blue Assisted Electrochemical Sensor for Determination of Drug Resistance of Escherichia coliRongshuai Duan0Rongshuai Duan1Xiao Fang2Dongliang Wang3Dongliang Wang4Department of Food and Drugs, Shandong Institute of Commerce and Technology, Jinan, ChinaQilu Medical University, Jinan, ChinaDepartment of Food and Drugs, Shandong Institute of Commerce and Technology, Jinan, ChinaDepartment of Food and Drugs, Shandong Institute of Commerce and Technology, Jinan, ChinaDong-E E-Jiao Co. Ltd., Liaocheng, ChinaDue to the abuse of antibiotics in clinical, animal husbandry, and aquaculture, drug-resistant pathogens are produced, which poses a great threat to human and the public health. At present, a rapid and effective drug sensitivity test method is urgently needed to effectively control the spread of drug-resistant bacteria. Using methylene blue as a redox probe, the electrochemical signals of methylene blue in drug-resistant Escherichia coli strains were analyzed by a CV method. Graphene ink has been used for enhancing the electrochemical signal. Compared with the results of the traditional drug sensitivity test, we proposed a rapid electrochemical drug sensitivity test method which can effectively identify the drug sensitivity of Escherichia coli. The sensitivity of four E. coli isolates to ciprofloxacin, gentamicin, and ampicillin was tested by an electrochemical drug sensitivity test. The respiratory activity value %RA was used as an indicator of bacterial resistance by electrochemical method.https://www.frontiersin.org/articles/10.3389/fchem.2021.689735/fullgraphenedrug resistantEscherichia coliglassy carbon electrodemethylene blue |
| spellingShingle | Rongshuai Duan Rongshuai Duan Xiao Fang Dongliang Wang Dongliang Wang A Methylene Blue Assisted Electrochemical Sensor for Determination of Drug Resistance of Escherichia coli Frontiers in Chemistry graphene drug resistant Escherichia coli glassy carbon electrode methylene blue |
| title | A Methylene Blue Assisted Electrochemical Sensor for Determination of Drug Resistance of Escherichia coli |
| title_full | A Methylene Blue Assisted Electrochemical Sensor for Determination of Drug Resistance of Escherichia coli |
| title_fullStr | A Methylene Blue Assisted Electrochemical Sensor for Determination of Drug Resistance of Escherichia coli |
| title_full_unstemmed | A Methylene Blue Assisted Electrochemical Sensor for Determination of Drug Resistance of Escherichia coli |
| title_short | A Methylene Blue Assisted Electrochemical Sensor for Determination of Drug Resistance of Escherichia coli |
| title_sort | methylene blue assisted electrochemical sensor for determination of drug resistance of escherichia coli |
| topic | graphene drug resistant Escherichia coli glassy carbon electrode methylene blue |
| url | https://www.frontiersin.org/articles/10.3389/fchem.2021.689735/full |
| work_keys_str_mv | AT rongshuaiduan amethyleneblueassistedelectrochemicalsensorfordeterminationofdrugresistanceofescherichiacoli AT rongshuaiduan amethyleneblueassistedelectrochemicalsensorfordeterminationofdrugresistanceofescherichiacoli AT xiaofang amethyleneblueassistedelectrochemicalsensorfordeterminationofdrugresistanceofescherichiacoli AT dongliangwang amethyleneblueassistedelectrochemicalsensorfordeterminationofdrugresistanceofescherichiacoli AT dongliangwang amethyleneblueassistedelectrochemicalsensorfordeterminationofdrugresistanceofescherichiacoli AT rongshuaiduan methyleneblueassistedelectrochemicalsensorfordeterminationofdrugresistanceofescherichiacoli AT rongshuaiduan methyleneblueassistedelectrochemicalsensorfordeterminationofdrugresistanceofescherichiacoli AT xiaofang methyleneblueassistedelectrochemicalsensorfordeterminationofdrugresistanceofescherichiacoli AT dongliangwang methyleneblueassistedelectrochemicalsensorfordeterminationofdrugresistanceofescherichiacoli AT dongliangwang methyleneblueassistedelectrochemicalsensorfordeterminationofdrugresistanceofescherichiacoli |