Agro-morphological traits and SSR markers reveal genetic variations in germplasm accessions of Indian mustard – An industrially important oilseed crop
Indian mustard is an economic and highly important industrial oilseed crop. In this study, genetic diversity among 135 Indian mustard germplasm accessions was evaluated using 11 agro-morphological descriptors and 227 SSRs. Morphological characterization of Indian mustard germplasm accessions exhibit...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-12-01
|
| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844022038075 |
| _version_ | 1797960806877364224 |
|---|---|
| author | Lal Singh J. Nanjundan Deepika Sharma K.H. Singh Nehanjali Parmar Rohit Jain Ajay Kumar Thakur |
| author_facet | Lal Singh J. Nanjundan Deepika Sharma K.H. Singh Nehanjali Parmar Rohit Jain Ajay Kumar Thakur |
| author_sort | Lal Singh |
| collection | DOAJ |
| description | Indian mustard is an economic and highly important industrial oilseed crop. In this study, genetic diversity among 135 Indian mustard germplasm accessions was evaluated using 11 agro-morphological descriptors and 227 SSRs. Morphological characterization of Indian mustard germplasm accessions exhibited a broad range of variation for characters including biological yield (CV = 25.63%), seed yield (CV = 23.23% and 1000-seed weight (CV = 23.14%); whereas traits such as days to maturity (CV = 2.91%) showed lowest degree of variation. Out of 227 SSR markers evaluated, a total of 159 (70.04%) SSRs produced polymorphic products and 68 (29.96%) SSRs resulted into monomorphic amplicons. The polymorphic markers amplified 575 alleles and the number of alleles ranged from 2-7 with 3.61 average number of alleles per locus. SSR markers BRMS-030, Ra2-E11, Ra2-G05, Ni4-G10 and Ol10B11 generated the highest number of alleles (7). SSR marker Ra2-G05 was having the highest allele frequency (0.84), while BRMS-002 was having the lowest major allele frequency (0.33). Polymorphism information content (PIC) values ranged from 0.24-0.61 with an average value of 0.39 per primer pair. Unweighted pair group method with arithmetic mean (UPGMA) cluster analysis based on morphological traits grouped Indian mustard genotypes into three clusters, while two clusters were obtained based on SSR based clustering. Population structure analysis provided a better estimate of genetic diversity and divided all the genotypes into five subpopulations. Genetically diverse accessions identified may be used for hybridization in Indian mustard crop improvement programs in future. |
| first_indexed | 2024-04-11T00:50:00Z |
| format | Article |
| id | doaj.art-57294218cd0c434e9d2f0b543358e4e6 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-11T00:50:00Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-57294218cd0c434e9d2f0b543358e4e62023-01-05T08:40:49ZengElsevierHeliyon2405-84402022-12-01812e12519Agro-morphological traits and SSR markers reveal genetic variations in germplasm accessions of Indian mustard – An industrially important oilseed cropLal Singh0J. Nanjundan1Deepika Sharma2K.H. Singh3Nehanjali Parmar4Rohit Jain5Ajay Kumar Thakur6Manipal University Jaipur, 303 007, Rajasthan, IndiaICAR-Indian Agricultural Research Institute-Regional Station, Wellington, 643 231, Tamilnadu, IndiaICAR-Directorate of Rapeseed-Mustard Research, Bharatpur, 321 303, Rajasthan, IndiaICAR-Directorate of Rapeseed-Mustard Research, Bharatpur, 321 303, Rajasthan, IndiaICAR-Directorate of Rapeseed-Mustard Research, Bharatpur, 321 303, Rajasthan, IndiaManipal University Jaipur, 303 007, Rajasthan, IndiaICAR-Directorate of Rapeseed-Mustard Research, Bharatpur, 321 303, Rajasthan, India; Corresponding author.Indian mustard is an economic and highly important industrial oilseed crop. In this study, genetic diversity among 135 Indian mustard germplasm accessions was evaluated using 11 agro-morphological descriptors and 227 SSRs. Morphological characterization of Indian mustard germplasm accessions exhibited a broad range of variation for characters including biological yield (CV = 25.63%), seed yield (CV = 23.23% and 1000-seed weight (CV = 23.14%); whereas traits such as days to maturity (CV = 2.91%) showed lowest degree of variation. Out of 227 SSR markers evaluated, a total of 159 (70.04%) SSRs produced polymorphic products and 68 (29.96%) SSRs resulted into monomorphic amplicons. The polymorphic markers amplified 575 alleles and the number of alleles ranged from 2-7 with 3.61 average number of alleles per locus. SSR markers BRMS-030, Ra2-E11, Ra2-G05, Ni4-G10 and Ol10B11 generated the highest number of alleles (7). SSR marker Ra2-G05 was having the highest allele frequency (0.84), while BRMS-002 was having the lowest major allele frequency (0.33). Polymorphism information content (PIC) values ranged from 0.24-0.61 with an average value of 0.39 per primer pair. Unweighted pair group method with arithmetic mean (UPGMA) cluster analysis based on morphological traits grouped Indian mustard genotypes into three clusters, while two clusters were obtained based on SSR based clustering. Population structure analysis provided a better estimate of genetic diversity and divided all the genotypes into five subpopulations. Genetically diverse accessions identified may be used for hybridization in Indian mustard crop improvement programs in future.http://www.sciencedirect.com/science/article/pii/S2405844022038075Brassica junceaGenetic diversityAgro-morphological traitsSSR markersPopulation structure |
| spellingShingle | Lal Singh J. Nanjundan Deepika Sharma K.H. Singh Nehanjali Parmar Rohit Jain Ajay Kumar Thakur Agro-morphological traits and SSR markers reveal genetic variations in germplasm accessions of Indian mustard – An industrially important oilseed crop Heliyon Brassica juncea Genetic diversity Agro-morphological traits SSR markers Population structure |
| title | Agro-morphological traits and SSR markers reveal genetic variations in germplasm accessions of Indian mustard – An industrially important oilseed crop |
| title_full | Agro-morphological traits and SSR markers reveal genetic variations in germplasm accessions of Indian mustard – An industrially important oilseed crop |
| title_fullStr | Agro-morphological traits and SSR markers reveal genetic variations in germplasm accessions of Indian mustard – An industrially important oilseed crop |
| title_full_unstemmed | Agro-morphological traits and SSR markers reveal genetic variations in germplasm accessions of Indian mustard – An industrially important oilseed crop |
| title_short | Agro-morphological traits and SSR markers reveal genetic variations in germplasm accessions of Indian mustard – An industrially important oilseed crop |
| title_sort | agro morphological traits and ssr markers reveal genetic variations in germplasm accessions of indian mustard an industrially important oilseed crop |
| topic | Brassica juncea Genetic diversity Agro-morphological traits SSR markers Population structure |
| url | http://www.sciencedirect.com/science/article/pii/S2405844022038075 |
| work_keys_str_mv | AT lalsingh agromorphologicaltraitsandssrmarkersrevealgeneticvariationsingermplasmaccessionsofindianmustardanindustriallyimportantoilseedcrop AT jnanjundan agromorphologicaltraitsandssrmarkersrevealgeneticvariationsingermplasmaccessionsofindianmustardanindustriallyimportantoilseedcrop AT deepikasharma agromorphologicaltraitsandssrmarkersrevealgeneticvariationsingermplasmaccessionsofindianmustardanindustriallyimportantoilseedcrop AT khsingh agromorphologicaltraitsandssrmarkersrevealgeneticvariationsingermplasmaccessionsofindianmustardanindustriallyimportantoilseedcrop AT nehanjaliparmar agromorphologicaltraitsandssrmarkersrevealgeneticvariationsingermplasmaccessionsofindianmustardanindustriallyimportantoilseedcrop AT rohitjain agromorphologicaltraitsandssrmarkersrevealgeneticvariationsingermplasmaccessionsofindianmustardanindustriallyimportantoilseedcrop AT ajaykumarthakur agromorphologicaltraitsandssrmarkersrevealgeneticvariationsingermplasmaccessionsofindianmustardanindustriallyimportantoilseedcrop |