The pleiotropic effects of atorvastatin on stable angina patients: Evidence by analysis of high-density lipoprotein size and subclasses, and plasma mRNA
Background: High-density lipoproteins (HDL) have atheroprotective biological properties: antioxidative, anti-apoptotic, anti-inflammatory, and they have the efflux capacity of cellular cholesterol. Plasma mRNA analysis can be used to investigate statin pleiotropy in vivo as a new analytical tool for...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Society of Medical Biochemists of Serbia, Belgrade
2015-01-01
|
| Series: | Journal of Medical Biochemistry |
| Subjects: | |
| Online Access: | https://scindeks-clanci.ceon.rs/data/pdf/1452-8258/2015/1452-82581503314M.pdf |
| _version_ | 1818693344642990080 |
|---|---|
| author | Mirjanić-Azarić Bosa Jelić-Ivanović Zorana Zeljković Aleksandra Vekić Jelena Jürgensć Günther Milivojac Tatjana Avram Sanja Ćorić Jozo Marc Janja Černe Darko |
| author_facet | Mirjanić-Azarić Bosa Jelić-Ivanović Zorana Zeljković Aleksandra Vekić Jelena Jürgensć Günther Milivojac Tatjana Avram Sanja Ćorić Jozo Marc Janja Černe Darko |
| author_sort | Mirjanić-Azarić Bosa |
| collection | DOAJ |
| description | Background: High-density lipoproteins (HDL) have atheroprotective biological properties: antioxidative, anti-apoptotic, anti-inflammatory, and they have the efflux capacity of cellular cholesterol. Plasma mRNA analysis can be used to investigate statin pleiotropy in vivo as a new analytical tool for non-invasive assessment of gene expression in vascular beds. The aim of this study was to assess the pleiotropic effects of atorvastatin in stable angina patients with high-risk values (group A) as compared with patients who had borderline and desirable HDL-cholesterol (HDL-C) values (group B). Methods: The atorvastatin therapy (20 mg/day) was given to forty-three patients with stable angina for 10 weeks. We investigated three statin pleiotropy-targeted genes: intercellular adhesion molecule-1, chemokine (C-C motif) ligand 2 and cathepsin S and assessed by gel electrophoresis gradient the effects of atorvastatin on HDL size and subclasses. Results: In group A, after therapy, HDL-C concentration was significantly increased but not in group B. Atorvastatin lowered plasma chemokine (C-C motif) lig- and 2 and intercellular adhesion molecule-1 mRNA levels in both groups, but did not change the plasma cathepsin S mRNA levels. In group A only, baseline total bilirubin showed negative correlations with the genes of cathepsin S (r = -0.506; p = 0.023) and significantly increased after therapy. Conclusions: HDL-C and bilirubin can be promising therapeutic targets in the treatment of cardiovascular diseases. Analysis of cell-free mRNA in plasma might become a useful tool for estimating statin pleiotropy. |
| first_indexed | 2024-12-17T13:12:12Z |
| format | Article |
| id | doaj.art-572c27a798414b3380a2ef0d7be4eda2 |
| institution | Directory Open Access Journal |
| issn | 1452-8258 1452-8266 |
| language | English |
| last_indexed | 2024-12-17T13:12:12Z |
| publishDate | 2015-01-01 |
| publisher | Society of Medical Biochemists of Serbia, Belgrade |
| record_format | Article |
| series | Journal of Medical Biochemistry |
| spelling | doaj.art-572c27a798414b3380a2ef0d7be4eda22022-12-21T21:47:05ZengSociety of Medical Biochemists of Serbia, BelgradeJournal of Medical Biochemistry1452-82581452-82662015-01-013433143221452-82581503314MThe pleiotropic effects of atorvastatin on stable angina patients: Evidence by analysis of high-density lipoprotein size and subclasses, and plasma mRNAMirjanić-Azarić Bosa0Jelić-Ivanović Zorana1https://orcid.org/0000-0002-3791-1743Zeljković Aleksandra2https://orcid.org/0000-0001-6417-8404Vekić Jelena3https://orcid.org/0000-0001-7445-0504Jürgensć Günther4Milivojac Tatjana5Avram Sanja6Ćorić Jozo7Marc Janja8Černe Darko9Clinical Centre Banja Luka, Department of Laboratory Diagnostics, Bosnia and Herzegovina + University of Ljubljana, Faculty of Pharmacy, SloveniaUniversity of Belgrade, Faculty of PharmacyUniversity of Belgrade, Faculty of PharmacyUniversity of Belgrade, Faculty of PharmacyMedical University of Graz, Institute of Physiological Chemistry, AustriaUniversity of Banja Luka, Medical Faculty, Bosnia and HerzegovinaClinical Centre Banja Luka, Department of Laboratory Diagnostics, Bosnia and HerzegovinaUniversity of Sarajevo, Clinical Center, Department of Clinical Chemistry and Biochemistry, Bosnia and HerzegovinaUniversity of Ljubljana, Faculty of Pharmacy, SloveniaUniversity of Ljubljana, Faculty of Pharmacy, SloveniaBackground: High-density lipoproteins (HDL) have atheroprotective biological properties: antioxidative, anti-apoptotic, anti-inflammatory, and they have the efflux capacity of cellular cholesterol. Plasma mRNA analysis can be used to investigate statin pleiotropy in vivo as a new analytical tool for non-invasive assessment of gene expression in vascular beds. The aim of this study was to assess the pleiotropic effects of atorvastatin in stable angina patients with high-risk values (group A) as compared with patients who had borderline and desirable HDL-cholesterol (HDL-C) values (group B). Methods: The atorvastatin therapy (20 mg/day) was given to forty-three patients with stable angina for 10 weeks. We investigated three statin pleiotropy-targeted genes: intercellular adhesion molecule-1, chemokine (C-C motif) ligand 2 and cathepsin S and assessed by gel electrophoresis gradient the effects of atorvastatin on HDL size and subclasses. Results: In group A, after therapy, HDL-C concentration was significantly increased but not in group B. Atorvastatin lowered plasma chemokine (C-C motif) lig- and 2 and intercellular adhesion molecule-1 mRNA levels in both groups, but did not change the plasma cathepsin S mRNA levels. In group A only, baseline total bilirubin showed negative correlations with the genes of cathepsin S (r = -0.506; p = 0.023) and significantly increased after therapy. Conclusions: HDL-C and bilirubin can be promising therapeutic targets in the treatment of cardiovascular diseases. Analysis of cell-free mRNA in plasma might become a useful tool for estimating statin pleiotropy.https://scindeks-clanci.ceon.rs/data/pdf/1452-8258/2015/1452-82581503314M.pdfhigh-density lipoprotein subclassescathepsin sbilirubinmrna in plasmaatorvastatin |
| spellingShingle | Mirjanić-Azarić Bosa Jelić-Ivanović Zorana Zeljković Aleksandra Vekić Jelena Jürgensć Günther Milivojac Tatjana Avram Sanja Ćorić Jozo Marc Janja Černe Darko The pleiotropic effects of atorvastatin on stable angina patients: Evidence by analysis of high-density lipoprotein size and subclasses, and plasma mRNA Journal of Medical Biochemistry high-density lipoprotein subclasses cathepsin s bilirubin mrna in plasma atorvastatin |
| title | The pleiotropic effects of atorvastatin on stable angina patients: Evidence by analysis of high-density lipoprotein size and subclasses, and plasma mRNA |
| title_full | The pleiotropic effects of atorvastatin on stable angina patients: Evidence by analysis of high-density lipoprotein size and subclasses, and plasma mRNA |
| title_fullStr | The pleiotropic effects of atorvastatin on stable angina patients: Evidence by analysis of high-density lipoprotein size and subclasses, and plasma mRNA |
| title_full_unstemmed | The pleiotropic effects of atorvastatin on stable angina patients: Evidence by analysis of high-density lipoprotein size and subclasses, and plasma mRNA |
| title_short | The pleiotropic effects of atorvastatin on stable angina patients: Evidence by analysis of high-density lipoprotein size and subclasses, and plasma mRNA |
| title_sort | pleiotropic effects of atorvastatin on stable angina patients evidence by analysis of high density lipoprotein size and subclasses and plasma mrna |
| topic | high-density lipoprotein subclasses cathepsin s bilirubin mrna in plasma atorvastatin |
| url | https://scindeks-clanci.ceon.rs/data/pdf/1452-8258/2015/1452-82581503314M.pdf |
| work_keys_str_mv | AT mirjanicazaricbosa thepleiotropiceffectsofatorvastatinonstableanginapatientsevidencebyanalysisofhighdensitylipoproteinsizeandsubclassesandplasmamrna AT jelicivanoviczorana thepleiotropiceffectsofatorvastatinonstableanginapatientsevidencebyanalysisofhighdensitylipoproteinsizeandsubclassesandplasmamrna AT zeljkovicaleksandra thepleiotropiceffectsofatorvastatinonstableanginapatientsevidencebyanalysisofhighdensitylipoproteinsizeandsubclassesandplasmamrna AT vekicjelena thepleiotropiceffectsofatorvastatinonstableanginapatientsevidencebyanalysisofhighdensitylipoproteinsizeandsubclassesandplasmamrna AT jurgenscgunther thepleiotropiceffectsofatorvastatinonstableanginapatientsevidencebyanalysisofhighdensitylipoproteinsizeandsubclassesandplasmamrna AT milivojactatjana thepleiotropiceffectsofatorvastatinonstableanginapatientsevidencebyanalysisofhighdensitylipoproteinsizeandsubclassesandplasmamrna AT avramsanja thepleiotropiceffectsofatorvastatinonstableanginapatientsevidencebyanalysisofhighdensitylipoproteinsizeandsubclassesandplasmamrna AT coricjozo thepleiotropiceffectsofatorvastatinonstableanginapatientsevidencebyanalysisofhighdensitylipoproteinsizeandsubclassesandplasmamrna AT marcjanja thepleiotropiceffectsofatorvastatinonstableanginapatientsevidencebyanalysisofhighdensitylipoproteinsizeandsubclassesandplasmamrna AT cernedarko thepleiotropiceffectsofatorvastatinonstableanginapatientsevidencebyanalysisofhighdensitylipoproteinsizeandsubclassesandplasmamrna AT mirjanicazaricbosa pleiotropiceffectsofatorvastatinonstableanginapatientsevidencebyanalysisofhighdensitylipoproteinsizeandsubclassesandplasmamrna AT jelicivanoviczorana pleiotropiceffectsofatorvastatinonstableanginapatientsevidencebyanalysisofhighdensitylipoproteinsizeandsubclassesandplasmamrna AT zeljkovicaleksandra pleiotropiceffectsofatorvastatinonstableanginapatientsevidencebyanalysisofhighdensitylipoproteinsizeandsubclassesandplasmamrna AT vekicjelena pleiotropiceffectsofatorvastatinonstableanginapatientsevidencebyanalysisofhighdensitylipoproteinsizeandsubclassesandplasmamrna AT jurgenscgunther pleiotropiceffectsofatorvastatinonstableanginapatientsevidencebyanalysisofhighdensitylipoproteinsizeandsubclassesandplasmamrna AT milivojactatjana pleiotropiceffectsofatorvastatinonstableanginapatientsevidencebyanalysisofhighdensitylipoproteinsizeandsubclassesandplasmamrna AT avramsanja pleiotropiceffectsofatorvastatinonstableanginapatientsevidencebyanalysisofhighdensitylipoproteinsizeandsubclassesandplasmamrna AT coricjozo pleiotropiceffectsofatorvastatinonstableanginapatientsevidencebyanalysisofhighdensitylipoproteinsizeandsubclassesandplasmamrna AT marcjanja pleiotropiceffectsofatorvastatinonstableanginapatientsevidencebyanalysisofhighdensitylipoproteinsizeandsubclassesandplasmamrna AT cernedarko pleiotropiceffectsofatorvastatinonstableanginapatientsevidencebyanalysisofhighdensitylipoproteinsizeandsubclassesandplasmamrna |