Black Cumin Seeds Extract Increase Lymphocyte Activity in IFN-γ Secretion in Sprague Dawley Rat (SD) Induced by Dimethylbenzantracene
Interferon-gamma (IFN-γ) is one of the central cytokines in the anti-carcinogenesis immune response. Black cumin seeds (BCS) have an active content of thymoquinone and unsaturated fatty acids with biological activity as immunomodulators. This study aimed to determine the effect of administration of...
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| Format: | Article |
| Language: | English |
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Indonesian Society for Cancer Chemoprevention
2019-12-01
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| Series: | ISCC (Indonesian Journal of Cancer Chemoprevention) |
| Online Access: | https://ijcc.chemoprev.org/index.php/ijcc/article/view/298 |
| _version_ | 1818243067142995968 |
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| author | Akrom Akrom Titiek Hidayati Sagiran Sagiran Indrayanti Indrayanti |
| author_facet | Akrom Akrom Titiek Hidayati Sagiran Sagiran Indrayanti Indrayanti |
| author_sort | Akrom Akrom |
| collection | DOAJ |
| description | Interferon-gamma (IFN-γ) is one of the central cytokines in the anti-carcinogenesis immune response. Black cumin seeds (BCS) have an active content of thymoquinone and unsaturated fatty acids with biological activity as immunomodulators. This study aimed to determine the effect of administration of BCS extract on IFN-γ secretion activity by DMBA-induced SD rat lymphocytes. In vivo experimental study on DMBA-induced SD rats, BCS extract was given with three doses for two weeks before being induced and five weeks during DMBA induction. IFN-γ levels in lymphocyte culture supernatants were determined by the ELISA method. The difference in IFN-γ levels between groups was analyzed by ANOVA test, the significance of 95%. The results showed that administration of BCS extract for 14 days did not affect cellular composition toward the edge of the test animal. BCS extract can increase IFN-γ secretion activity by DMBA-induced SD rat lymphocytes.
Keywords: black cumin seed, IFN-γ; DMBA: immunomodulator, carcinogenesis. |
| first_indexed | 2024-12-12T13:55:14Z |
| format | Article |
| id | doaj.art-572c447d80ba44ffab5e8b82f753bae9 |
| institution | Directory Open Access Journal |
| issn | 2088-0197 2355-8989 |
| language | English |
| last_indexed | 2024-12-12T13:55:14Z |
| publishDate | 2019-12-01 |
| publisher | Indonesian Society for Cancer Chemoprevention |
| record_format | Article |
| series | ISCC (Indonesian Journal of Cancer Chemoprevention) |
| spelling | doaj.art-572c447d80ba44ffab5e8b82f753bae92022-12-22T00:22:28ZengIndonesian Society for Cancer ChemopreventionISCC (Indonesian Journal of Cancer Chemoprevention)2088-01972355-89892019-12-0110314014810.14499/indonesianjcanchemoprev10iss3pp140-148193Black Cumin Seeds Extract Increase Lymphocyte Activity in IFN-γ Secretion in Sprague Dawley Rat (SD) Induced by DimethylbenzantraceneAkrom Akrom0Titiek Hidayati1Sagiran Sagiran2Indrayanti Indrayanti3Pharmacology and Clinical Pharmacy, Pharmacy Faculty, Universitas Ahmad DahlanCommunity and Family medicine Department, Medicine and Health Science Faculty, Universitas Muhammadiyah Yogyakarta, Yogyakarta, IndonesiaSurgeon Department, Medicine and Health Science Faculty, Universitas Muhammadiyah Yogyakarta, Yogyakarta, IndonesiaPathology anatomy Department, Medicine and Health Science Faculty, Universitas Muhammadiyah Yogyakarta, Yogyakarta, IndonesiaInterferon-gamma (IFN-γ) is one of the central cytokines in the anti-carcinogenesis immune response. Black cumin seeds (BCS) have an active content of thymoquinone and unsaturated fatty acids with biological activity as immunomodulators. This study aimed to determine the effect of administration of BCS extract on IFN-γ secretion activity by DMBA-induced SD rat lymphocytes. In vivo experimental study on DMBA-induced SD rats, BCS extract was given with three doses for two weeks before being induced and five weeks during DMBA induction. IFN-γ levels in lymphocyte culture supernatants were determined by the ELISA method. The difference in IFN-γ levels between groups was analyzed by ANOVA test, the significance of 95%. The results showed that administration of BCS extract for 14 days did not affect cellular composition toward the edge of the test animal. BCS extract can increase IFN-γ secretion activity by DMBA-induced SD rat lymphocytes. Keywords: black cumin seed, IFN-γ; DMBA: immunomodulator, carcinogenesis.https://ijcc.chemoprev.org/index.php/ijcc/article/view/298 |
| spellingShingle | Akrom Akrom Titiek Hidayati Sagiran Sagiran Indrayanti Indrayanti Black Cumin Seeds Extract Increase Lymphocyte Activity in IFN-γ Secretion in Sprague Dawley Rat (SD) Induced by Dimethylbenzantracene ISCC (Indonesian Journal of Cancer Chemoprevention) |
| title | Black Cumin Seeds Extract Increase Lymphocyte Activity in IFN-γ Secretion in Sprague Dawley Rat (SD) Induced by Dimethylbenzantracene |
| title_full | Black Cumin Seeds Extract Increase Lymphocyte Activity in IFN-γ Secretion in Sprague Dawley Rat (SD) Induced by Dimethylbenzantracene |
| title_fullStr | Black Cumin Seeds Extract Increase Lymphocyte Activity in IFN-γ Secretion in Sprague Dawley Rat (SD) Induced by Dimethylbenzantracene |
| title_full_unstemmed | Black Cumin Seeds Extract Increase Lymphocyte Activity in IFN-γ Secretion in Sprague Dawley Rat (SD) Induced by Dimethylbenzantracene |
| title_short | Black Cumin Seeds Extract Increase Lymphocyte Activity in IFN-γ Secretion in Sprague Dawley Rat (SD) Induced by Dimethylbenzantracene |
| title_sort | black cumin seeds extract increase lymphocyte activity in ifn γ secretion in sprague dawley rat sd induced by dimethylbenzantracene |
| url | https://ijcc.chemoprev.org/index.php/ijcc/article/view/298 |
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