A novel intradermal tattoo-based injection device enhances the immunogenicity of plasmid DNA vaccines
Abstract In recent years, tattooing technology has shown promising results toward evaluating vaccines in both animal models and humans. However, this technology has some limitations due to variability of experimental evaluations or operator procedures. The current study evaluated a device (intraderm...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-12-01
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| Series: | npj Vaccines |
| Online Access: | https://doi.org/10.1038/s41541-022-00581-y |
| _version_ | 1797422429126000640 |
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| author | Alejandro M. Gomez George (Giorgi) Babuadze Marc-André Plourde-Campagna Hiva Azizi Alice Berger Robert Kozak Marc-Antoine de La Vega Ara XIII Maedeh Naghibosadat Marie-Edith Nepveu-Traversy Jean Ruel Gary P. Kobinger |
| author_facet | Alejandro M. Gomez George (Giorgi) Babuadze Marc-André Plourde-Campagna Hiva Azizi Alice Berger Robert Kozak Marc-Antoine de La Vega Ara XIII Maedeh Naghibosadat Marie-Edith Nepveu-Traversy Jean Ruel Gary P. Kobinger |
| author_sort | Alejandro M. Gomez |
| collection | DOAJ |
| description | Abstract In recent years, tattooing technology has shown promising results toward evaluating vaccines in both animal models and humans. However, this technology has some limitations due to variability of experimental evaluations or operator procedures. The current study evaluated a device (intradermal oscillating needle array injection device: IONAID) capable of microinjecting a controlled dose of any aqueous vaccine into the intradermal space. IONAID-mediated administration of a DNA-based vaccine encoding the glycoprotein (GP) from the Ebola virus resulted in superior T- and B-cell responses with IONAID when compared to single intramuscular (IM) or intradermal (ID) injection in mice. Moreover, humoral immune responses, induced after IONAID vaccination, were significantly higher to those obtained with traditional passive DNA tattooing in guinea pigs and rabbits. This device was well tolerated and safe during HIV vaccine delivery in non-human primates (NHPs), while inducing robust immune responses. In summary, this study shows that the IONAID device improves vaccine performance, which could be beneficial to the animal and human health, and importantly, provide a dose-sparing approach (e.g., monkeypox vaccine). |
| first_indexed | 2024-03-09T07:32:10Z |
| format | Article |
| id | doaj.art-572cc9afa15541998f90ad9f819ab826 |
| institution | Directory Open Access Journal |
| issn | 2059-0105 |
| language | English |
| last_indexed | 2024-03-09T07:32:10Z |
| publishDate | 2022-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Vaccines |
| spelling | doaj.art-572cc9afa15541998f90ad9f819ab8262023-12-03T05:53:42ZengNature Portfolionpj Vaccines2059-01052022-12-017111010.1038/s41541-022-00581-yA novel intradermal tattoo-based injection device enhances the immunogenicity of plasmid DNA vaccinesAlejandro M. Gomez0George (Giorgi) Babuadze1Marc-André Plourde-Campagna2Hiva Azizi3Alice Berger4Robert Kozak5Marc-Antoine de La Vega6Ara XIII7Maedeh Naghibosadat8Marie-Edith Nepveu-Traversy9Jean Ruel10Gary P. Kobinger11Département de Microbiologie-Infectiologie et Immunologie, Faculté de Médecine, Université LavalBiological Sciences Platform, University Toronto, Sunnybrook Research Institute at Sunnybrook Health Sciences CentreDépartement de Génie Mécanique, Université LavalDépartement de Microbiologie-Infectiologie et Immunologie, Faculté de Médecine, Université LavalDépartement de Microbiologie-Infectiologie et Immunologie, Faculté de Médecine, Université LavalBiological Sciences Platform, University Toronto, Sunnybrook Research Institute at Sunnybrook Health Sciences CentreDepartment of Microbiology and Immunology, University of Texas Medical BranchDepartment of Microbiology and Immunology, University of Texas Medical BranchBiological Sciences Platform, University Toronto, Sunnybrook Research Institute at Sunnybrook Health Sciences CentreGlobal Urgent and Advanced Research and Development (GuardRX)Département de Génie Mécanique, Université LavalDepartment of Microbiology and Immunology, University of Texas Medical BranchAbstract In recent years, tattooing technology has shown promising results toward evaluating vaccines in both animal models and humans. However, this technology has some limitations due to variability of experimental evaluations or operator procedures. The current study evaluated a device (intradermal oscillating needle array injection device: IONAID) capable of microinjecting a controlled dose of any aqueous vaccine into the intradermal space. IONAID-mediated administration of a DNA-based vaccine encoding the glycoprotein (GP) from the Ebola virus resulted in superior T- and B-cell responses with IONAID when compared to single intramuscular (IM) or intradermal (ID) injection in mice. Moreover, humoral immune responses, induced after IONAID vaccination, were significantly higher to those obtained with traditional passive DNA tattooing in guinea pigs and rabbits. This device was well tolerated and safe during HIV vaccine delivery in non-human primates (NHPs), while inducing robust immune responses. In summary, this study shows that the IONAID device improves vaccine performance, which could be beneficial to the animal and human health, and importantly, provide a dose-sparing approach (e.g., monkeypox vaccine).https://doi.org/10.1038/s41541-022-00581-y |
| spellingShingle | Alejandro M. Gomez George (Giorgi) Babuadze Marc-André Plourde-Campagna Hiva Azizi Alice Berger Robert Kozak Marc-Antoine de La Vega Ara XIII Maedeh Naghibosadat Marie-Edith Nepveu-Traversy Jean Ruel Gary P. Kobinger A novel intradermal tattoo-based injection device enhances the immunogenicity of plasmid DNA vaccines npj Vaccines |
| title | A novel intradermal tattoo-based injection device enhances the immunogenicity of plasmid DNA vaccines |
| title_full | A novel intradermal tattoo-based injection device enhances the immunogenicity of plasmid DNA vaccines |
| title_fullStr | A novel intradermal tattoo-based injection device enhances the immunogenicity of plasmid DNA vaccines |
| title_full_unstemmed | A novel intradermal tattoo-based injection device enhances the immunogenicity of plasmid DNA vaccines |
| title_short | A novel intradermal tattoo-based injection device enhances the immunogenicity of plasmid DNA vaccines |
| title_sort | novel intradermal tattoo based injection device enhances the immunogenicity of plasmid dna vaccines |
| url | https://doi.org/10.1038/s41541-022-00581-y |
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