A Prospective Study of the Serological, Clinical, and Epidemiological Features of a SARS-CoV-2 Positive Pediatric Cohort
Background: SARS-CoV-2 was a global pandemic. Children develop a mild disease and may have a different rate of seroconversion compared to adults. The objective was to determine the number of seronegative patients in a pediatric cohort. We also reviewed the clinical–epidemiological features associate...
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| Language: | English |
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MDPI AG
2022-05-01
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| Series: | Children |
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| Online Access: | https://www.mdpi.com/2227-9067/9/5/665 |
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| author | Ignacio Callejas-Caballero Alba Ruedas-López Arantxa Berzosa-Sánchez Marta Illán-Ramos Belén Joyanes-Abancens Andrés Bodas-Pinedo Sara Guillén-Martín Beatriz Soto-Sánchez Isabel García-Bermejo David Molina-Arana Juan-Ignacio Alós Elvira Baos-Muñoz Alberto Delgado-Iribarren Manuel E. Fuentes-Ferrer José T. Ramos-Amador |
| author_facet | Ignacio Callejas-Caballero Alba Ruedas-López Arantxa Berzosa-Sánchez Marta Illán-Ramos Belén Joyanes-Abancens Andrés Bodas-Pinedo Sara Guillén-Martín Beatriz Soto-Sánchez Isabel García-Bermejo David Molina-Arana Juan-Ignacio Alós Elvira Baos-Muñoz Alberto Delgado-Iribarren Manuel E. Fuentes-Ferrer José T. Ramos-Amador |
| author_sort | Ignacio Callejas-Caballero |
| collection | DOAJ |
| description | Background: SARS-CoV-2 was a global pandemic. Children develop a mild disease and may have a different rate of seroconversion compared to adults. The objective was to determine the number of seronegative patients in a pediatric cohort. We also reviewed the clinical–epidemiological features associated with seroconversion. Methods: A multicenter prospective observational study during September–November 2020, of COVID-19, confirmed by reverse transcription-polymerase chain reaction. Data were obtained 4–8 weeks after diagnosis. Blood samples were collected to investigate the humoral response, using three different serological methods. Results: A total of 111 patients were included (98 symptomatic), 8 were admitted to hospital, none required an Intensive Care Unit visit. Median age: 88 months (IQR: 24–149). Median time between diagnosis and serological test: 37 days (IQR: 34–44). A total of 19 patients were non-seroconverters when using three serological techniques (17.1%; 95% CI: 10.6–25.4); most were aged 2–10 years (35%, <i>p</i> < 0.05). Univariate analysis yielded a lower rate of seroconversion when COVID-19 confirmation was not present amongst household contacts (51.7%; <i>p</i> < 0.05). Conclusions: There was a high proportion of non-seroconverters. This is more commonly encountered in childhood than in adults. Most seronegative patients were in the group aged 2–10 years, and when COVID-19 was not documented in household contacts. Most developed a mild disease. Frequently, children were not the index case within the family. |
| first_indexed | 2024-03-10T03:08:35Z |
| format | Article |
| id | doaj.art-572d75fa6d5942f18dc6c382bbdb51d8 |
| institution | Directory Open Access Journal |
| issn | 2227-9067 |
| language | English |
| last_indexed | 2024-03-10T03:08:35Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Children |
| spelling | doaj.art-572d75fa6d5942f18dc6c382bbdb51d82023-11-23T10:30:36ZengMDPI AGChildren2227-90672022-05-019566510.3390/children9050665A Prospective Study of the Serological, Clinical, and Epidemiological Features of a SARS-CoV-2 Positive Pediatric CohortIgnacio Callejas-Caballero0Alba Ruedas-López1Arantxa Berzosa-Sánchez2Marta Illán-Ramos3Belén Joyanes-Abancens4Andrés Bodas-Pinedo5Sara Guillén-Martín6Beatriz Soto-Sánchez7Isabel García-Bermejo8David Molina-Arana9Juan-Ignacio Alós10Elvira Baos-Muñoz11Alberto Delgado-Iribarren12Manuel E. Fuentes-Ferrer13José T. Ramos-Amador14Department of Paediatrics, Collaborator at Instituto de Investigación Sanitaria del Hospital Universitario Clínico San Carlos (IdISSC), 28040 Madrid, SpainDepartment of Microbiology, Collaborator at Instituto de Investigación Sanitaria del Hospital Universitario Clínico San Carlos (IdISSC), 28040 Madrid, SpainDepartment of Paediatrics, Collaborator at Instituto de Investigación Sanitaria del Hospital Universitario Clínico San Carlos (IdISSC), 28040 Madrid, SpainDepartment of Paediatrics, Collaborator at Instituto de Investigación Sanitaria del Hospital Universitario Clínico San Carlos (IdISSC), 28040 Madrid, SpainDepartment of Paediatrics, Collaborator at Instituto de Investigación Sanitaria del Hospital Universitario Clínico San Carlos (IdISSC), 28040 Madrid, SpainDepartment of Paediatrics, Collaborator at Instituto de Investigación Sanitaria del Hospital Universitario Clínico San Carlos (IdISSC), 28040 Madrid, SpainDepartment of Paediatrics, Hospital Universitario de Getafe, 28905 Madrid, SpainDepartment of Paediatrics, Hospital Universitario de Getafe, 28905 Madrid, SpainDepartment of Microbiology, Hospital Universitario de Getafe, 28905 Madrid, SpainDepartment of Microbiology, Hospital Universitario de Getafe, 28905 Madrid, SpainDepartment of Microbiology, Hospital Universitario de Getafe, 28905 Madrid, SpainDepartment of Microbiology, Collaborator at Instituto de Investigación Sanitaria del Hospital Universitario Clínico San Carlos (IdISSC), 28040 Madrid, SpainDepartment of Microbiology, Collaborator at Instituto de Investigación Sanitaria del Hospital Universitario Clínico San Carlos (IdISSC), 28040 Madrid, SpainStatistician at Hospital Universitario Clínico San Carlos, 28040 Madrid, SpainDepartment of Paediatrics, Universidad Complutense-Instituto de Investigación Sanitaria del Hospital Universitario Clínico San Carlos (IdISSC), 28040 Madrid, SpainBackground: SARS-CoV-2 was a global pandemic. Children develop a mild disease and may have a different rate of seroconversion compared to adults. The objective was to determine the number of seronegative patients in a pediatric cohort. We also reviewed the clinical–epidemiological features associated with seroconversion. Methods: A multicenter prospective observational study during September–November 2020, of COVID-19, confirmed by reverse transcription-polymerase chain reaction. Data were obtained 4–8 weeks after diagnosis. Blood samples were collected to investigate the humoral response, using three different serological methods. Results: A total of 111 patients were included (98 symptomatic), 8 were admitted to hospital, none required an Intensive Care Unit visit. Median age: 88 months (IQR: 24–149). Median time between diagnosis and serological test: 37 days (IQR: 34–44). A total of 19 patients were non-seroconverters when using three serological techniques (17.1%; 95% CI: 10.6–25.4); most were aged 2–10 years (35%, <i>p</i> < 0.05). Univariate analysis yielded a lower rate of seroconversion when COVID-19 confirmation was not present amongst household contacts (51.7%; <i>p</i> < 0.05). Conclusions: There was a high proportion of non-seroconverters. This is more commonly encountered in childhood than in adults. Most seronegative patients were in the group aged 2–10 years, and when COVID-19 was not documented in household contacts. Most developed a mild disease. Frequently, children were not the index case within the family.https://www.mdpi.com/2227-9067/9/5/665RT-PCR (reverse transcriptase polymerase chain reaction)SARS-CoV-2COVID-19seroconversionchildren |
| spellingShingle | Ignacio Callejas-Caballero Alba Ruedas-López Arantxa Berzosa-Sánchez Marta Illán-Ramos Belén Joyanes-Abancens Andrés Bodas-Pinedo Sara Guillén-Martín Beatriz Soto-Sánchez Isabel García-Bermejo David Molina-Arana Juan-Ignacio Alós Elvira Baos-Muñoz Alberto Delgado-Iribarren Manuel E. Fuentes-Ferrer José T. Ramos-Amador A Prospective Study of the Serological, Clinical, and Epidemiological Features of a SARS-CoV-2 Positive Pediatric Cohort Children RT-PCR (reverse transcriptase polymerase chain reaction) SARS-CoV-2 COVID-19 seroconversion children |
| title | A Prospective Study of the Serological, Clinical, and Epidemiological Features of a SARS-CoV-2 Positive Pediatric Cohort |
| title_full | A Prospective Study of the Serological, Clinical, and Epidemiological Features of a SARS-CoV-2 Positive Pediatric Cohort |
| title_fullStr | A Prospective Study of the Serological, Clinical, and Epidemiological Features of a SARS-CoV-2 Positive Pediatric Cohort |
| title_full_unstemmed | A Prospective Study of the Serological, Clinical, and Epidemiological Features of a SARS-CoV-2 Positive Pediatric Cohort |
| title_short | A Prospective Study of the Serological, Clinical, and Epidemiological Features of a SARS-CoV-2 Positive Pediatric Cohort |
| title_sort | prospective study of the serological clinical and epidemiological features of a sars cov 2 positive pediatric cohort |
| topic | RT-PCR (reverse transcriptase polymerase chain reaction) SARS-CoV-2 COVID-19 seroconversion children |
| url | https://www.mdpi.com/2227-9067/9/5/665 |
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