Field Performance and Genetic Stability of Micropropagated Gooseberry Plants (<i>Ribes grossularia</i> L.)
Gooseberry (<i>Ribes grossularia</i> L.) is a small fruit crop producing valuable fruits, which is constantly gaining importance. In vitro propagation of this species can significantly support the production of virus-free planting material and accelerate the introduction of new cultivars...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-12-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/11/1/45 |
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| author | Danuta Wójcik Aleksandra Trzewik Danuta Kucharska |
| author_facet | Danuta Wójcik Aleksandra Trzewik Danuta Kucharska |
| author_sort | Danuta Wójcik |
| collection | DOAJ |
| description | Gooseberry (<i>Ribes grossularia</i> L.) is a small fruit crop producing valuable fruits, which is constantly gaining importance. In vitro propagation of this species can significantly support the production of virus-free planting material and accelerate the introduction of new cultivars to the market. The aim of presented study was to assess field performance and genetic stability of micropropagated plants (MPs) of four gooseberry cultivars, “Captivator”, “Hinnonmaki Rot”, “Invicta”, and “Resika”. The growth vigor and yield of MPs and plants propagated by standard methods from softwood cuttings (ST) were evaluated in a field experiment. Microscopic observations of the number and length of the stomata of MP and ST plants were carried out. Two DNA-based techniques, amplified fragment length polymorphism (AFLP) and inter simple sequence repeat (ISSR), were used to assess genetic stability of MP plants. For analysis of genetic stability of ST plants, the ISSR technique was applied. For three cultivars, Captivator, Hinnonmaki Rot, and Invicta, the plants’ growth vigor and fruit yield were greater in MP plants than in ST plants. In the case of Resika, most of these parameters were higher in ST plants. Microscopic observations of the stomata indicated a lack of differences in the length between MP and ST plants, while the stomata frequency on leaves of MP plants was higher than that of ST plants. The genetic variability of MP plants, assessed by AFLP, ranged from 0.35% for Hinnonmaki Rot to 2.12% for Resika. The results of ISSR analysis of MP plants showed variability from 0% in the case of Hinnonmaki Rot and Resika to 4% and 8.69% for Captivator and Invicta, respectively. No polymorphism was detected among ST plants of all analyzed gooseberry cultivars. |
| first_indexed | 2024-03-10T13:42:34Z |
| format | Article |
| id | doaj.art-572e04c9291542719e63031b2d6c6ad5 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T13:42:34Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-572e04c9291542719e63031b2d6c6ad52023-11-21T02:51:35ZengMDPI AGAgronomy2073-43952020-12-011114510.3390/agronomy11010045Field Performance and Genetic Stability of Micropropagated Gooseberry Plants (<i>Ribes grossularia</i> L.)Danuta Wójcik0Aleksandra Trzewik1Danuta Kucharska2Department of Applied Biology, Research Institute of Horticulture, Konstytucji 3 Maja 1/3 Str., 96-100 Skierniewice, PolandDepartment of Applied Biology, Research Institute of Horticulture, Konstytucji 3 Maja 1/3 Str., 96-100 Skierniewice, PolandDepartment of Applied Biology, Research Institute of Horticulture, Konstytucji 3 Maja 1/3 Str., 96-100 Skierniewice, PolandGooseberry (<i>Ribes grossularia</i> L.) is a small fruit crop producing valuable fruits, which is constantly gaining importance. In vitro propagation of this species can significantly support the production of virus-free planting material and accelerate the introduction of new cultivars to the market. The aim of presented study was to assess field performance and genetic stability of micropropagated plants (MPs) of four gooseberry cultivars, “Captivator”, “Hinnonmaki Rot”, “Invicta”, and “Resika”. The growth vigor and yield of MPs and plants propagated by standard methods from softwood cuttings (ST) were evaluated in a field experiment. Microscopic observations of the number and length of the stomata of MP and ST plants were carried out. Two DNA-based techniques, amplified fragment length polymorphism (AFLP) and inter simple sequence repeat (ISSR), were used to assess genetic stability of MP plants. For analysis of genetic stability of ST plants, the ISSR technique was applied. For three cultivars, Captivator, Hinnonmaki Rot, and Invicta, the plants’ growth vigor and fruit yield were greater in MP plants than in ST plants. In the case of Resika, most of these parameters were higher in ST plants. Microscopic observations of the stomata indicated a lack of differences in the length between MP and ST plants, while the stomata frequency on leaves of MP plants was higher than that of ST plants. The genetic variability of MP plants, assessed by AFLP, ranged from 0.35% for Hinnonmaki Rot to 2.12% for Resika. The results of ISSR analysis of MP plants showed variability from 0% in the case of Hinnonmaki Rot and Resika to 4% and 8.69% for Captivator and Invicta, respectively. No polymorphism was detected among ST plants of all analyzed gooseberry cultivars.https://www.mdpi.com/2073-4395/11/1/45gooseberrymicropropagationsomaclonal variationmolecular markersAFLPISSR |
| spellingShingle | Danuta Wójcik Aleksandra Trzewik Danuta Kucharska Field Performance and Genetic Stability of Micropropagated Gooseberry Plants (<i>Ribes grossularia</i> L.) Agronomy gooseberry micropropagation somaclonal variation molecular markers AFLP ISSR |
| title | Field Performance and Genetic Stability of Micropropagated Gooseberry Plants (<i>Ribes grossularia</i> L.) |
| title_full | Field Performance and Genetic Stability of Micropropagated Gooseberry Plants (<i>Ribes grossularia</i> L.) |
| title_fullStr | Field Performance and Genetic Stability of Micropropagated Gooseberry Plants (<i>Ribes grossularia</i> L.) |
| title_full_unstemmed | Field Performance and Genetic Stability of Micropropagated Gooseberry Plants (<i>Ribes grossularia</i> L.) |
| title_short | Field Performance and Genetic Stability of Micropropagated Gooseberry Plants (<i>Ribes grossularia</i> L.) |
| title_sort | field performance and genetic stability of micropropagated gooseberry plants i ribes grossularia i l |
| topic | gooseberry micropropagation somaclonal variation molecular markers AFLP ISSR |
| url | https://www.mdpi.com/2073-4395/11/1/45 |
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