Rapid diagnosis of Zika virus through saliva and urine by Loop-mediated isothermal amplification (LAMP)
Background: Zika virus (ZIKV) is a single-stranded RNA virus and member of the Flaviviridae family. Recent studies have reported that saliva can be an important alternative to detect ZIKV. Saliva requires less processing than blood greatly simplifying the assay. Loop-mediated Isothermal Amplificatio...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-01-01
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| Series: | Journal of Oral Microbiology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/20002297.2018.1510712 |
| _version_ | 1818549432861327360 |
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| author | Talita Castro Maite Sabalza Cheryl Barber William Abrams Antonio Charlys Da Costa Flavio Augusto De Pádua Milagres Paulo Henrique Braz-Silva Daniel Malamud Marina Gallottini |
| author_facet | Talita Castro Maite Sabalza Cheryl Barber William Abrams Antonio Charlys Da Costa Flavio Augusto De Pádua Milagres Paulo Henrique Braz-Silva Daniel Malamud Marina Gallottini |
| author_sort | Talita Castro |
| collection | DOAJ |
| description | Background: Zika virus (ZIKV) is a single-stranded RNA virus and member of the Flaviviridae family. Recent studies have reported that saliva can be an important alternative to detect ZIKV. Saliva requires less processing than blood greatly simplifying the assay. Loop-mediated Isothermal Amplification (LAMP) is a rapid assay that detects nucleic acids, including ZIKV RNA. Aim: The aim of this study was to evaluate the efficacy of saliva and urine to diagnose ZIKV infection in subjects during the acute phase, through ZIKV RNA detection by LAMP. Method: A total of 131 samples (68 saliva and 63 urine samples) from 69 subjects in the acute phase of ZIKV infection, and confirmed positive for ZIKV by blood analysis through real time-PCR, were collected and analyzed by Reverse Transcriptase Loop-mediated Isothermal Amplification (RT-LAMP). Results: From the 68 saliva samples, 45 (66.2%) were positive for ZIKV with an average time to positivity (Tp) of 13.5 min, and from the 63 urine samples, 25 (39.7%) were positive with the average Tp of 15.8 min. Saliva detected more samples (p = 0.0042) and had faster Tp (p = 0.0176) as compared with urine. Conclusion: Saliva proved to be a feasible alternative to diagnose ZIKV infection during the acute phase by LAMP. |
| first_indexed | 2024-12-12T08:33:16Z |
| format | Article |
| id | doaj.art-572eb1c1b3154d55b9e317c75a50089d |
| institution | Directory Open Access Journal |
| issn | 2000-2297 |
| language | English |
| last_indexed | 2024-12-12T08:33:16Z |
| publishDate | 2018-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Oral Microbiology |
| spelling | doaj.art-572eb1c1b3154d55b9e317c75a50089d2022-12-22T00:31:01ZengTaylor & Francis GroupJournal of Oral Microbiology2000-22972018-01-0110110.1080/20002297.2018.15107121510712Rapid diagnosis of Zika virus through saliva and urine by Loop-mediated isothermal amplification (LAMP)Talita Castro0Maite Sabalza1Cheryl Barber2William Abrams3Antonio Charlys Da Costa4Flavio Augusto De Pádua Milagres5Paulo Henrique Braz-Silva6Daniel Malamud7Marina Gallottini8University of São PauloCollege of Dentistry, New York UniversityCollege of Dentistry, New York UniversityCollege of Dentistry, New York UniversityInstitute of Tropical Medicine, University of São PauloSecretary of Health of Tocantins and Federal University of TocantinsUniversity of São PauloCollege of Dentistry, New York UniversityUniversity of São PauloBackground: Zika virus (ZIKV) is a single-stranded RNA virus and member of the Flaviviridae family. Recent studies have reported that saliva can be an important alternative to detect ZIKV. Saliva requires less processing than blood greatly simplifying the assay. Loop-mediated Isothermal Amplification (LAMP) is a rapid assay that detects nucleic acids, including ZIKV RNA. Aim: The aim of this study was to evaluate the efficacy of saliva and urine to diagnose ZIKV infection in subjects during the acute phase, through ZIKV RNA detection by LAMP. Method: A total of 131 samples (68 saliva and 63 urine samples) from 69 subjects in the acute phase of ZIKV infection, and confirmed positive for ZIKV by blood analysis through real time-PCR, were collected and analyzed by Reverse Transcriptase Loop-mediated Isothermal Amplification (RT-LAMP). Results: From the 68 saliva samples, 45 (66.2%) were positive for ZIKV with an average time to positivity (Tp) of 13.5 min, and from the 63 urine samples, 25 (39.7%) were positive with the average Tp of 15.8 min. Saliva detected more samples (p = 0.0042) and had faster Tp (p = 0.0176) as compared with urine. Conclusion: Saliva proved to be a feasible alternative to diagnose ZIKV infection during the acute phase by LAMP.http://dx.doi.org/10.1080/20002297.2018.1510712Flavivirusvirologypolymerase chain reactionRNAinfectionDenguesalivaLAMPZika |
| spellingShingle | Talita Castro Maite Sabalza Cheryl Barber William Abrams Antonio Charlys Da Costa Flavio Augusto De Pádua Milagres Paulo Henrique Braz-Silva Daniel Malamud Marina Gallottini Rapid diagnosis of Zika virus through saliva and urine by Loop-mediated isothermal amplification (LAMP) Journal of Oral Microbiology Flavivirus virology polymerase chain reaction RNA infection Dengue saliva LAMP Zika |
| title | Rapid diagnosis of Zika virus through saliva and urine by Loop-mediated isothermal amplification (LAMP) |
| title_full | Rapid diagnosis of Zika virus through saliva and urine by Loop-mediated isothermal amplification (LAMP) |
| title_fullStr | Rapid diagnosis of Zika virus through saliva and urine by Loop-mediated isothermal amplification (LAMP) |
| title_full_unstemmed | Rapid diagnosis of Zika virus through saliva and urine by Loop-mediated isothermal amplification (LAMP) |
| title_short | Rapid diagnosis of Zika virus through saliva and urine by Loop-mediated isothermal amplification (LAMP) |
| title_sort | rapid diagnosis of zika virus through saliva and urine by loop mediated isothermal amplification lamp |
| topic | Flavivirus virology polymerase chain reaction RNA infection Dengue saliva LAMP Zika |
| url | http://dx.doi.org/10.1080/20002297.2018.1510712 |
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