TRI Genotyping and Chemotyping: A Balance of Power
<i>Fusarium</i><i> </i>is among the top 10 most economically important plant pathogens in the world.<b> </b>Trichothecenes are the principal mycotoxins produced as secondary metabolites by select species of <i>Fusarium</i> and cause acute and chronic t...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-01-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/12/2/64 |
| _version_ | 1817992533571010560 |
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| author | Ria T. Villafana Amanda C. Ramdass Sephra N. Rampersad |
| author_facet | Ria T. Villafana Amanda C. Ramdass Sephra N. Rampersad |
| author_sort | Ria T. Villafana |
| collection | DOAJ |
| description | <i>Fusarium</i><i> </i>is among the top 10 most economically important plant pathogens in the world.<b> </b>Trichothecenes are the principal mycotoxins produced as secondary metabolites by select species of <i>Fusarium</i> and cause acute and chronic toxicity in animals and humans upon exposure either through consumption and/or contact.<b> </b>There are over 100<b> </b>trichothecene metabolites and they can occur in a wide range of commodities that form food and feed products. This review discusses strategies to mitigate the risk of mycotoxin production and exposure by examining the <i>Fusarium</i>-trichothecene model. Fundamental to mitigation of risk is knowing the identity of the pathogen. As such, a comparison of current, recommended molecular approaches for sequence-based identification of Fusaria is presented, followed by an analysis of the rationale and methods of trichothecene (TRI) genotyping and chemotyping. This type of information confirms the source and nature of risk. While both are powerful tools for informing regulatory decisions, an assessment of the causes of incongruence between TRI genotyping and chemotyping data must be made. Reconciliation of this discordance will map the way forward in terms of optimization of molecular approaches, which includes data validation and sharing in the form of accessible repositories of genomic data and browsers for querying such data. |
| first_indexed | 2024-04-14T01:27:08Z |
| format | Article |
| id | doaj.art-572eebe6c6fc4ceb99ba58af04946ed6 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-14T01:27:08Z |
| publishDate | 2020-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-572eebe6c6fc4ceb99ba58af04946ed62022-12-22T02:20:21ZengMDPI AGToxins2072-66512020-01-011226410.3390/toxins12020064toxins12020064TRI Genotyping and Chemotyping: A Balance of PowerRia T. Villafana0Amanda C. Ramdass1Sephra N. Rampersad2Dept. of Life Sciences, Faculty of Science and Technology, The University of the West Indies, St. Augustine, Trinidad and TobagoDept. of Life Sciences, Faculty of Science and Technology, The University of the West Indies, St. Augustine, Trinidad and TobagoDept. of Life Sciences, Faculty of Science and Technology, The University of the West Indies, St. Augustine, Trinidad and Tobago<i>Fusarium</i><i> </i>is among the top 10 most economically important plant pathogens in the world.<b> </b>Trichothecenes are the principal mycotoxins produced as secondary metabolites by select species of <i>Fusarium</i> and cause acute and chronic toxicity in animals and humans upon exposure either through consumption and/or contact.<b> </b>There are over 100<b> </b>trichothecene metabolites and they can occur in a wide range of commodities that form food and feed products. This review discusses strategies to mitigate the risk of mycotoxin production and exposure by examining the <i>Fusarium</i>-trichothecene model. Fundamental to mitigation of risk is knowing the identity of the pathogen. As such, a comparison of current, recommended molecular approaches for sequence-based identification of Fusaria is presented, followed by an analysis of the rationale and methods of trichothecene (TRI) genotyping and chemotyping. This type of information confirms the source and nature of risk. While both are powerful tools for informing regulatory decisions, an assessment of the causes of incongruence between TRI genotyping and chemotyping data must be made. Reconciliation of this discordance will map the way forward in terms of optimization of molecular approaches, which includes data validation and sharing in the form of accessible repositories of genomic data and browsers for querying such data.https://www.mdpi.com/2072-6651/12/2/64genotypingchemotypingfusariummolecular detectiontrichothecenes |
| spellingShingle | Ria T. Villafana Amanda C. Ramdass Sephra N. Rampersad TRI Genotyping and Chemotyping: A Balance of Power Toxins genotyping chemotyping fusarium molecular detection trichothecenes |
| title | TRI Genotyping and Chemotyping: A Balance of Power |
| title_full | TRI Genotyping and Chemotyping: A Balance of Power |
| title_fullStr | TRI Genotyping and Chemotyping: A Balance of Power |
| title_full_unstemmed | TRI Genotyping and Chemotyping: A Balance of Power |
| title_short | TRI Genotyping and Chemotyping: A Balance of Power |
| title_sort | tri genotyping and chemotyping a balance of power |
| topic | genotyping chemotyping fusarium molecular detection trichothecenes |
| url | https://www.mdpi.com/2072-6651/12/2/64 |
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