MR Spectroscopy of the Insula: Within- and between-Session Reproducibility of MEGA-PRESS Measurements of GABA+ and Other Metabolites
The insula plays a critical role in many neuropsychological disorders. Research investigating its neurochemistry with magnetic resonance spectroscopy (MRS) has been limited compared with cortical regions. Here, we investigate the within-session and between-session reproducibility of metabolite measu...
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| Format: | Article |
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MDPI AG
2021-11-01
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| Series: | Brain Sciences |
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| Online Access: | https://www.mdpi.com/2076-3425/11/11/1538 |
| _version_ | 1797511003252981760 |
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| author | Claire Shyu Sonja Elsaid Peter Truong Sofia Chavez Bernard Le Foll |
| author_facet | Claire Shyu Sonja Elsaid Peter Truong Sofia Chavez Bernard Le Foll |
| author_sort | Claire Shyu |
| collection | DOAJ |
| description | The insula plays a critical role in many neuropsychological disorders. Research investigating its neurochemistry with magnetic resonance spectroscopy (MRS) has been limited compared with cortical regions. Here, we investigate the within-session and between-session reproducibility of metabolite measurements in the insula on a 3T scanner. We measure N-acetylaspartate + N-acetylaspartylglutamate (tNAA), creatine + phosphocreatine (tCr), glycerophosphocholine + phosphocholine (tCho), myo-inositol (Ins), glutamate + glutamine (Glx), and γ-aminobutyric acid (GABA) in one cohort using a j-edited MEGA-PRESS sequence. We measure tNAA, tCr, tCho, Ins, and Glx in another cohort with a standard short-TE PRESS sequence as a reference for the reproducibility metrics. All participants were scanned 4 times identically: 2 back-to-back scans each day, on 2 days. Preprocessing was done using LCModel and Gannet. Reproducibility was determined using Pearson’s <i>r</i>, intraclass-correlation coefficients (ICC), coefficients of variation (CV%), and Bland–Altman plots. A MEGA-PRESS protocol requiring averaged results over two 6:45-min scans yielded reproducible GABA measurements (CV% = 7.15%). This averaging also yielded reproducibility metrics comparable to those from PRESS for the other metabolites. Voxel placement inconsistencies did not affect reproducibility, and no sex differences were found. The data suggest that MEGA-PRESS can reliably measure standard metabolites and GABA in the insula. |
| first_indexed | 2024-03-10T05:39:16Z |
| format | Article |
| id | doaj.art-5734595edaeb47c59e6e84174fe60112 |
| institution | Directory Open Access Journal |
| issn | 2076-3425 |
| language | English |
| last_indexed | 2024-03-10T05:39:16Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Brain Sciences |
| spelling | doaj.art-5734595edaeb47c59e6e84174fe601122023-11-22T22:39:09ZengMDPI AGBrain Sciences2076-34252021-11-011111153810.3390/brainsci11111538MR Spectroscopy of the Insula: Within- and between-Session Reproducibility of MEGA-PRESS Measurements of GABA+ and Other MetabolitesClaire Shyu0Sonja Elsaid1Peter Truong2Sofia Chavez3Bernard Le Foll4Translational Addiction Research Laboratory, Centre for Addiction and Mental Health, University of Toronto, Toronto, ON M5S 2S1, CanadaTranslational Addiction Research Laboratory, Centre for Addiction and Mental Health, University of Toronto, Toronto, ON M5S 2S1, CanadaBrain Health Imaging Centre, Centre for Addiction and Mental Health, Toronto, ON M5T 1R8, CanadaBrain Health Imaging Centre, Centre for Addiction and Mental Health, Toronto, ON M5T 1R8, CanadaTranslational Addiction Research Laboratory, Centre for Addiction and Mental Health, University of Toronto, Toronto, ON M5S 2S1, CanadaThe insula plays a critical role in many neuropsychological disorders. Research investigating its neurochemistry with magnetic resonance spectroscopy (MRS) has been limited compared with cortical regions. Here, we investigate the within-session and between-session reproducibility of metabolite measurements in the insula on a 3T scanner. We measure N-acetylaspartate + N-acetylaspartylglutamate (tNAA), creatine + phosphocreatine (tCr), glycerophosphocholine + phosphocholine (tCho), myo-inositol (Ins), glutamate + glutamine (Glx), and γ-aminobutyric acid (GABA) in one cohort using a j-edited MEGA-PRESS sequence. We measure tNAA, tCr, tCho, Ins, and Glx in another cohort with a standard short-TE PRESS sequence as a reference for the reproducibility metrics. All participants were scanned 4 times identically: 2 back-to-back scans each day, on 2 days. Preprocessing was done using LCModel and Gannet. Reproducibility was determined using Pearson’s <i>r</i>, intraclass-correlation coefficients (ICC), coefficients of variation (CV%), and Bland–Altman plots. A MEGA-PRESS protocol requiring averaged results over two 6:45-min scans yielded reproducible GABA measurements (CV% = 7.15%). This averaging also yielded reproducibility metrics comparable to those from PRESS for the other metabolites. Voxel placement inconsistencies did not affect reproducibility, and no sex differences were found. The data suggest that MEGA-PRESS can reliably measure standard metabolites and GABA in the insula.https://www.mdpi.com/2076-3425/11/11/1538insulagamma-aminobutyric acidGABALCModelMEGA-PRESSPRESS |
| spellingShingle | Claire Shyu Sonja Elsaid Peter Truong Sofia Chavez Bernard Le Foll MR Spectroscopy of the Insula: Within- and between-Session Reproducibility of MEGA-PRESS Measurements of GABA+ and Other Metabolites Brain Sciences insula gamma-aminobutyric acid GABA LCModel MEGA-PRESS PRESS |
| title | MR Spectroscopy of the Insula: Within- and between-Session Reproducibility of MEGA-PRESS Measurements of GABA+ and Other Metabolites |
| title_full | MR Spectroscopy of the Insula: Within- and between-Session Reproducibility of MEGA-PRESS Measurements of GABA+ and Other Metabolites |
| title_fullStr | MR Spectroscopy of the Insula: Within- and between-Session Reproducibility of MEGA-PRESS Measurements of GABA+ and Other Metabolites |
| title_full_unstemmed | MR Spectroscopy of the Insula: Within- and between-Session Reproducibility of MEGA-PRESS Measurements of GABA+ and Other Metabolites |
| title_short | MR Spectroscopy of the Insula: Within- and between-Session Reproducibility of MEGA-PRESS Measurements of GABA+ and Other Metabolites |
| title_sort | mr spectroscopy of the insula within and between session reproducibility of mega press measurements of gaba and other metabolites |
| topic | insula gamma-aminobutyric acid GABA LCModel MEGA-PRESS PRESS |
| url | https://www.mdpi.com/2076-3425/11/11/1538 |
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