Aflatoxin B1 targeted gene expression profiles in human placental primary trophoblast cells
Aflatoxin B1 (AFB1) is a mycotoxin produced by Aspergillus flavus and A. parasiticus. A high exposure (40 nM and 1 µM AFB1 for 72 h) was used to study mechanistic effects of AFB1 on gene expression patterns in human primary trophoblast cells, isolated from full term placentae after delivery. Gene ex...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-01-01
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| Series: | Current Research in Toxicology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666027X22000196 |
| _version_ | 1828088523243126784 |
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| author | Rami El-Dairi Jaana Rysä Markus Storvik Markku Pasanen Pasi Huuskonen |
| author_facet | Rami El-Dairi Jaana Rysä Markus Storvik Markku Pasanen Pasi Huuskonen |
| author_sort | Rami El-Dairi |
| collection | DOAJ |
| description | Aflatoxin B1 (AFB1) is a mycotoxin produced by Aspergillus flavus and A. parasiticus. A high exposure (40 nM and 1 µM AFB1 for 72 h) was used to study mechanistic effects of AFB1 on gene expression patterns in human primary trophoblast cells, isolated from full term placentae after delivery. Gene expression profiling was conducted, and Ingenuity pathway analysis (IPA) software was used to identify AFB1-regulated gene networks and regulatory pathways.In response to 40 nM AFB1, only 7 genes were differentially expressed whereas 1 µM AFB1 significantly dysregulated 170 genes (124 down- and 46 upregulated, ±1.5-fold, p < 0.05) in AFB1-exposed trophoblasts when compared to controls. The top downregulated genes were involved in endocrine signalling and biosynthesis of hormones, and lipid and carbohydrate metabolism. The top upregulated genes were involved in protein synthesis and regulation of cell cycle. The main canonical pathways identified by IPA were associated with endocrine signalling including growth hormone signalling, and corticotropin releasing hormone signalling. Furthermore, genes involved in aryl hydrocarbon receptor (AhR)-mediated estrogen receptor signalling were dysregulated in response to AFB1.Our findings indicate that a high concentration 72 h AFB1 exposure caused relatively moderate number of changes on transcript level to human placental primary trophoblast cells. However, these preliminary results need to be confirmed with human-relevant concentrations of AFB1. |
| first_indexed | 2024-04-11T05:28:07Z |
| format | Article |
| id | doaj.art-5735d3ca688d46fbac4a16596f6242ba |
| institution | Directory Open Access Journal |
| issn | 2666-027X |
| language | English |
| last_indexed | 2024-04-11T05:28:07Z |
| publishDate | 2022-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Toxicology |
| spelling | doaj.art-5735d3ca688d46fbac4a16596f6242ba2022-12-23T04:42:22ZengElsevierCurrent Research in Toxicology2666-027X2022-01-013100082Aflatoxin B1 targeted gene expression profiles in human placental primary trophoblast cellsRami El-Dairi0Jaana Rysä1Markus Storvik2Markku Pasanen3Pasi Huuskonen4School of Pharmacy, Faculty of Health Sciences, University of Eastern Finland, FIN-70211 Kuopio, FinlandSchool of Pharmacy, Faculty of Health Sciences, University of Eastern Finland, FIN-70211 Kuopio, FinlandSchool of Pharmacy, Faculty of Health Sciences, University of Eastern Finland, FIN-70211 Kuopio, FinlandSchool of Pharmacy, Faculty of Health Sciences, University of Eastern Finland, FIN-70211 Kuopio, FinlandCorresponding author.; School of Pharmacy, Faculty of Health Sciences, University of Eastern Finland, FIN-70211 Kuopio, FinlandAflatoxin B1 (AFB1) is a mycotoxin produced by Aspergillus flavus and A. parasiticus. A high exposure (40 nM and 1 µM AFB1 for 72 h) was used to study mechanistic effects of AFB1 on gene expression patterns in human primary trophoblast cells, isolated from full term placentae after delivery. Gene expression profiling was conducted, and Ingenuity pathway analysis (IPA) software was used to identify AFB1-regulated gene networks and regulatory pathways.In response to 40 nM AFB1, only 7 genes were differentially expressed whereas 1 µM AFB1 significantly dysregulated 170 genes (124 down- and 46 upregulated, ±1.5-fold, p < 0.05) in AFB1-exposed trophoblasts when compared to controls. The top downregulated genes were involved in endocrine signalling and biosynthesis of hormones, and lipid and carbohydrate metabolism. The top upregulated genes were involved in protein synthesis and regulation of cell cycle. The main canonical pathways identified by IPA were associated with endocrine signalling including growth hormone signalling, and corticotropin releasing hormone signalling. Furthermore, genes involved in aryl hydrocarbon receptor (AhR)-mediated estrogen receptor signalling were dysregulated in response to AFB1.Our findings indicate that a high concentration 72 h AFB1 exposure caused relatively moderate number of changes on transcript level to human placental primary trophoblast cells. However, these preliminary results need to be confirmed with human-relevant concentrations of AFB1.http://www.sciencedirect.com/science/article/pii/S2666027X22000196Aflatoxin B1Estrogen signallingGene expression profilingPathway analysisPlacentaTrophoblast |
| spellingShingle | Rami El-Dairi Jaana Rysä Markus Storvik Markku Pasanen Pasi Huuskonen Aflatoxin B1 targeted gene expression profiles in human placental primary trophoblast cells Current Research in Toxicology Aflatoxin B1 Estrogen signalling Gene expression profiling Pathway analysis Placenta Trophoblast |
| title | Aflatoxin B1 targeted gene expression profiles in human placental primary trophoblast cells |
| title_full | Aflatoxin B1 targeted gene expression profiles in human placental primary trophoblast cells |
| title_fullStr | Aflatoxin B1 targeted gene expression profiles in human placental primary trophoblast cells |
| title_full_unstemmed | Aflatoxin B1 targeted gene expression profiles in human placental primary trophoblast cells |
| title_short | Aflatoxin B1 targeted gene expression profiles in human placental primary trophoblast cells |
| title_sort | aflatoxin b1 targeted gene expression profiles in human placental primary trophoblast cells |
| topic | Aflatoxin B1 Estrogen signalling Gene expression profiling Pathway analysis Placenta Trophoblast |
| url | http://www.sciencedirect.com/science/article/pii/S2666027X22000196 |
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