Mixed Diets Reduce the Oxidative Stress of Common Carp (Cyprinus carpio): Based on MicroRNA Sequencing
The rice-fish mode, a mode of ecological aquaculture, has become a popular research topic in recent years. The antioxidant capacity of fish can be affected by the type of diet. Three groups of adult common carp (initial weight 517.8 ± 50 g) were fed earthworm (group A), earthworm + duckweed (group M...
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Frontiers Media S.A.
2019-05-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphys.2019.00631/full |
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| author | Song Yang Jie Luo Yalan Long Jie Du GangChun Xu Liulan Zhao Zongjun Du Wei Luo Yan Wang Zhi He |
| author_facet | Song Yang Jie Luo Yalan Long Jie Du GangChun Xu Liulan Zhao Zongjun Du Wei Luo Yan Wang Zhi He |
| author_sort | Song Yang |
| collection | DOAJ |
| description | The rice-fish mode, a mode of ecological aquaculture, has become a popular research topic in recent years. The antioxidant capacity of fish can be affected by the type of diet. Three groups of adult common carp (initial weight 517.8 ± 50 g) were fed earthworm (group A), earthworm + duckweed (group M), and duckweed (group P). The antioxidant capacity of common carp (Cyprinus carpio) was evaluated by histopathological sectioning, antioxidant enzyme activity, and the miRNA transcriptome profile. The pathological changes in group M were lighter than those in groups C and A. The activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) significantly increased in group M, and the malondialdehyde content (MDA) significantly decreased (p < 0.05). Additionally, nine differentially expressed miRNAs (DEMs) were found between groups A and M, and eight DEMs found between groups P and M were identified in the liver of common carp. Five miRNAs were reported to be related to oxidative stress, including miR-137-3p, miR-143-3p, miR-146a-5p, miR-21-5p, and miR-125b-5p. Compared with group M, all five detected miRNAs were upregulated in group A, and four of the detected miRNAs were upregulated in group P. The targets of the five miRNAs were further predicted via functional analysis. Our study confirmed that omnivorous common carp exhibits stronger antioxidant capacity when feeding on both an animal diet and a plant diet. |
| first_indexed | 2024-12-10T12:04:40Z |
| format | Article |
| id | doaj.art-57389653c56c45ee89808a8cb725dcbc |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-10T12:04:40Z |
| publishDate | 2019-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-57389653c56c45ee89808a8cb725dcbc2022-12-22T01:49:30ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2019-05-011010.3389/fphys.2019.00631426977Mixed Diets Reduce the Oxidative Stress of Common Carp (Cyprinus carpio): Based on MicroRNA SequencingSong Yang0Jie Luo1Yalan Long2Jie Du3GangChun Xu4Liulan Zhao5Zongjun Du6Wei Luo7Yan Wang8Zhi He9College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, ChinaThe rice-fish mode, a mode of ecological aquaculture, has become a popular research topic in recent years. The antioxidant capacity of fish can be affected by the type of diet. Three groups of adult common carp (initial weight 517.8 ± 50 g) were fed earthworm (group A), earthworm + duckweed (group M), and duckweed (group P). The antioxidant capacity of common carp (Cyprinus carpio) was evaluated by histopathological sectioning, antioxidant enzyme activity, and the miRNA transcriptome profile. The pathological changes in group M were lighter than those in groups C and A. The activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) significantly increased in group M, and the malondialdehyde content (MDA) significantly decreased (p < 0.05). Additionally, nine differentially expressed miRNAs (DEMs) were found between groups A and M, and eight DEMs found between groups P and M were identified in the liver of common carp. Five miRNAs were reported to be related to oxidative stress, including miR-137-3p, miR-143-3p, miR-146a-5p, miR-21-5p, and miR-125b-5p. Compared with group M, all five detected miRNAs were upregulated in group A, and four of the detected miRNAs were upregulated in group P. The targets of the five miRNAs were further predicted via functional analysis. Our study confirmed that omnivorous common carp exhibits stronger antioxidant capacity when feeding on both an animal diet and a plant diet.https://www.frontiersin.org/article/10.3389/fphys.2019.00631/fullrice-fish modedietsecological aquacultureanimal welfaremicroRNA sequencingoxidative stress |
| spellingShingle | Song Yang Jie Luo Yalan Long Jie Du GangChun Xu Liulan Zhao Zongjun Du Wei Luo Yan Wang Zhi He Mixed Diets Reduce the Oxidative Stress of Common Carp (Cyprinus carpio): Based on MicroRNA Sequencing Frontiers in Physiology rice-fish mode diets ecological aquaculture animal welfare microRNA sequencing oxidative stress |
| title | Mixed Diets Reduce the Oxidative Stress of Common Carp (Cyprinus carpio): Based on MicroRNA Sequencing |
| title_full | Mixed Diets Reduce the Oxidative Stress of Common Carp (Cyprinus carpio): Based on MicroRNA Sequencing |
| title_fullStr | Mixed Diets Reduce the Oxidative Stress of Common Carp (Cyprinus carpio): Based on MicroRNA Sequencing |
| title_full_unstemmed | Mixed Diets Reduce the Oxidative Stress of Common Carp (Cyprinus carpio): Based on MicroRNA Sequencing |
| title_short | Mixed Diets Reduce the Oxidative Stress of Common Carp (Cyprinus carpio): Based on MicroRNA Sequencing |
| title_sort | mixed diets reduce the oxidative stress of common carp cyprinus carpio based on microrna sequencing |
| topic | rice-fish mode diets ecological aquaculture animal welfare microRNA sequencing oxidative stress |
| url | https://www.frontiersin.org/article/10.3389/fphys.2019.00631/full |
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