Na<sup>+</sup> Transporter SvHKT1;1 from a Halophytic Turf Grass Is Specifically Upregulated by High Na<sup>+</sup> Concentration and Regulates Shoot Na<sup>+</sup> Concentration
We characterized an Na<sup>+</sup> transporter SvHKT1;1 from a halophytic turf grass, <i>Sporobolus virginicus</i>. SvHKT1;1 mediated inward and outward Na<sup>+</sup> transport in <i>Xenopus laevis</i> oocytes and did not complement K<sup>+</...
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MDPI AG
2020-08-01
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| author | Yuki Kawakami Shahin Imran Maki Katsuhara Yuichi Tada |
| author_facet | Yuki Kawakami Shahin Imran Maki Katsuhara Yuichi Tada |
| author_sort | Yuki Kawakami |
| collection | DOAJ |
| description | We characterized an Na<sup>+</sup> transporter SvHKT1;1 from a halophytic turf grass, <i>Sporobolus virginicus</i>. SvHKT1;1 mediated inward and outward Na<sup>+</sup> transport in <i>Xenopus laevis</i> oocytes and did not complement K<sup>+</sup> transporter-defective mutant yeast. SvHKT1;1 did not complement <i>athkt1;1</i> mutant <i>Arabidopsis</i>, suggesting its distinguishable function from other typical HKT1 transporters. The transcript was abundant in the shoots compared with the roots in <i>S. virginicus</i> and was upregulated by severe salt stress (500 mM NaCl), but not by lower stress. <i>SvHKT1;1</i>-expressing <i>Arabidopsis</i> lines showed higher shoot Na<sup>+</sup> concentrations and lower salt tolerance than wild type (WT) plants under nonstress and salt stress conditions and showed higher Na<sup>+</sup> uptake rate in roots at the early stage of salt treatment. These results suggested that constitutive expression of <i>SvHKT1;1</i> enhanced Na<sup>+</sup> uptake in root epidermal cells, followed by increased Na<sup>+</sup> transport to shoots, which led to reduced salt tolerance. However, Na<sup>+</sup> concentrations in phloem sap of the SvHKT1;1 lines were higher than those in WT plants under salt stress. Based on this result, together with the induction of the <i>SvHKT1;1</i> transcription under high salinity stress, it was suggested that SvHKT1;1 plays a role in preventing excess shoot Na<sup>+</sup> accumulation in <i>S. virginicus</i>. |
| first_indexed | 2024-03-10T16:53:52Z |
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| spelling | doaj.art-5738fb367faa49cfb9c2c408624e51722023-11-20T11:13:22ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-08-012117610010.3390/ijms21176100Na<sup>+</sup> Transporter SvHKT1;1 from a Halophytic Turf Grass Is Specifically Upregulated by High Na<sup>+</sup> Concentration and Regulates Shoot Na<sup>+</sup> ConcentrationYuki Kawakami0Shahin Imran1Maki Katsuhara2Yuichi Tada3Graduate School of Bionics, Computer and Media Sciences, Tokyo University of Technology, 1404-1 Katakura, Hachioji, Tokyo 192-0982, JapanInstitute of Plant Science and Resources, Okayama University, Chuo 2-20-1, Kurashiki, Okayama 710-0046, JapanInstitute of Plant Science and Resources, Okayama University, Chuo 2-20-1, Kurashiki, Okayama 710-0046, JapanSchool of Biosciences and Biotechnology, Tokyo University of Technology, 1404-1 Katakura, Hachioji, Tokyo 192-0982, JapanWe characterized an Na<sup>+</sup> transporter SvHKT1;1 from a halophytic turf grass, <i>Sporobolus virginicus</i>. SvHKT1;1 mediated inward and outward Na<sup>+</sup> transport in <i>Xenopus laevis</i> oocytes and did not complement K<sup>+</sup> transporter-defective mutant yeast. SvHKT1;1 did not complement <i>athkt1;1</i> mutant <i>Arabidopsis</i>, suggesting its distinguishable function from other typical HKT1 transporters. The transcript was abundant in the shoots compared with the roots in <i>S. virginicus</i> and was upregulated by severe salt stress (500 mM NaCl), but not by lower stress. <i>SvHKT1;1</i>-expressing <i>Arabidopsis</i> lines showed higher shoot Na<sup>+</sup> concentrations and lower salt tolerance than wild type (WT) plants under nonstress and salt stress conditions and showed higher Na<sup>+</sup> uptake rate in roots at the early stage of salt treatment. These results suggested that constitutive expression of <i>SvHKT1;1</i> enhanced Na<sup>+</sup> uptake in root epidermal cells, followed by increased Na<sup>+</sup> transport to shoots, which led to reduced salt tolerance. However, Na<sup>+</sup> concentrations in phloem sap of the SvHKT1;1 lines were higher than those in WT plants under salt stress. Based on this result, together with the induction of the <i>SvHKT1;1</i> transcription under high salinity stress, it was suggested that SvHKT1;1 plays a role in preventing excess shoot Na<sup>+</sup> accumulation in <i>S. virginicus</i>.https://www.mdpi.com/1422-0067/21/17/6100<i>Arabidopsis thaliana</i>halophytehigh-affinity potassium transporter (HKT)Na<sup>+</sup> transportersalt tolerance<i>Sporobolus virginicus</i> |
| spellingShingle | Yuki Kawakami Shahin Imran Maki Katsuhara Yuichi Tada Na<sup>+</sup> Transporter SvHKT1;1 from a Halophytic Turf Grass Is Specifically Upregulated by High Na<sup>+</sup> Concentration and Regulates Shoot Na<sup>+</sup> Concentration International Journal of Molecular Sciences <i>Arabidopsis thaliana</i> halophyte high-affinity potassium transporter (HKT) Na<sup>+</sup> transporter salt tolerance <i>Sporobolus virginicus</i> |
| title | Na<sup>+</sup> Transporter SvHKT1;1 from a Halophytic Turf Grass Is Specifically Upregulated by High Na<sup>+</sup> Concentration and Regulates Shoot Na<sup>+</sup> Concentration |
| title_full | Na<sup>+</sup> Transporter SvHKT1;1 from a Halophytic Turf Grass Is Specifically Upregulated by High Na<sup>+</sup> Concentration and Regulates Shoot Na<sup>+</sup> Concentration |
| title_fullStr | Na<sup>+</sup> Transporter SvHKT1;1 from a Halophytic Turf Grass Is Specifically Upregulated by High Na<sup>+</sup> Concentration and Regulates Shoot Na<sup>+</sup> Concentration |
| title_full_unstemmed | Na<sup>+</sup> Transporter SvHKT1;1 from a Halophytic Turf Grass Is Specifically Upregulated by High Na<sup>+</sup> Concentration and Regulates Shoot Na<sup>+</sup> Concentration |
| title_short | Na<sup>+</sup> Transporter SvHKT1;1 from a Halophytic Turf Grass Is Specifically Upregulated by High Na<sup>+</sup> Concentration and Regulates Shoot Na<sup>+</sup> Concentration |
| title_sort | na sup sup transporter svhkt1 1 from a halophytic turf grass is specifically upregulated by high na sup sup concentration and regulates shoot na sup sup concentration |
| topic | <i>Arabidopsis thaliana</i> halophyte high-affinity potassium transporter (HKT) Na<sup>+</sup> transporter salt tolerance <i>Sporobolus virginicus</i> |
| url | https://www.mdpi.com/1422-0067/21/17/6100 |
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