Lectin-induced alterations of the interaction of insulin and insulin-like growth factor 1 receptors with their ligands
In order to study whether the carbohydrate moieties of the human placental IGF-I receptor (IGF1R), IGF-II receptor (IGF2R) and insulin receptors (IRs) play a role in ligand binding, solubilised cell membrane preparations were incubated with 125I-labelled IGF-I, IGF-II and insulin in the presence of...
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| Format: | Article |
| Language: | English |
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Serbian Chemical Society
2008-08-01
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| Series: | Journal of the Serbian Chemical Society |
| Subjects: | |
| Online Access: | http://www.shd.org.yu/JSCS/Vol73/No8-9/JSCS-3762.pdf |
| _version_ | 1811278913324711936 |
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| author | ROMANA MASNIKOSA ANNA J. NIKOLIC OLGICA NEDIC |
| author_facet | ROMANA MASNIKOSA ANNA J. NIKOLIC OLGICA NEDIC |
| author_sort | ROMANA MASNIKOSA |
| collection | DOAJ |
| description | In order to study whether the carbohydrate moieties of the human placental IGF-I receptor (IGF1R), IGF-II receptor (IGF2R) and insulin receptors (IRs) play a role in ligand binding, solubilised cell membrane preparations were incubated with 125I-labelled IGF-I, IGF-II and insulin in the presence of lectins with different sugar specificities. Three incubation procedures were tested: ligand-first, co-incubation and lectin-first incubation. Wheat germ agglutinin (WGA), concanavalin A (Con A) and phytohaemagglutinin (PHA) altered the binding of IGF-I and insulin to their high-affinity receptors in a lectin specific and dose-dependent manner, whereas these lectins did not affect the interaction of IGF-II with its receptor(s). Moreover, the same lectins either inhibited or enhanced IGF-I and insulin binding, depending on the incubation scheme. These results also suggest that IR-A and IR-B from human placenta might be differently glycosylated. |
| first_indexed | 2024-04-13T00:44:16Z |
| format | Article |
| id | doaj.art-5739ad0b486a42b0a5ab2034e59dfb09 |
| institution | Directory Open Access Journal |
| issn | 0352-5139 |
| language | English |
| last_indexed | 2024-04-13T00:44:16Z |
| publishDate | 2008-08-01 |
| publisher | Serbian Chemical Society |
| record_format | Article |
| series | Journal of the Serbian Chemical Society |
| spelling | doaj.art-5739ad0b486a42b0a5ab2034e59dfb092022-12-22T03:10:03ZengSerbian Chemical SocietyJournal of the Serbian Chemical Society0352-51392008-08-01738-9793804Lectin-induced alterations of the interaction of insulin and insulin-like growth factor 1 receptors with their ligandsROMANA MASNIKOSAANNA J. NIKOLICOLGICA NEDICIn order to study whether the carbohydrate moieties of the human placental IGF-I receptor (IGF1R), IGF-II receptor (IGF2R) and insulin receptors (IRs) play a role in ligand binding, solubilised cell membrane preparations were incubated with 125I-labelled IGF-I, IGF-II and insulin in the presence of lectins with different sugar specificities. Three incubation procedures were tested: ligand-first, co-incubation and lectin-first incubation. Wheat germ agglutinin (WGA), concanavalin A (Con A) and phytohaemagglutinin (PHA) altered the binding of IGF-I and insulin to their high-affinity receptors in a lectin specific and dose-dependent manner, whereas these lectins did not affect the interaction of IGF-II with its receptor(s). Moreover, the same lectins either inhibited or enhanced IGF-I and insulin binding, depending on the incubation scheme. These results also suggest that IR-A and IR-B from human placenta might be differently glycosylated.http://www.shd.org.yu/JSCS/Vol73/No8-9/JSCS-3762.pdfIGF1RIGF2RIRWGACon APHA |
| spellingShingle | ROMANA MASNIKOSA ANNA J. NIKOLIC OLGICA NEDIC Lectin-induced alterations of the interaction of insulin and insulin-like growth factor 1 receptors with their ligands Journal of the Serbian Chemical Society IGF1R IGF2R IR WGA Con A PHA |
| title | Lectin-induced alterations of the interaction of insulin and insulin-like growth factor 1 receptors with their ligands |
| title_full | Lectin-induced alterations of the interaction of insulin and insulin-like growth factor 1 receptors with their ligands |
| title_fullStr | Lectin-induced alterations of the interaction of insulin and insulin-like growth factor 1 receptors with their ligands |
| title_full_unstemmed | Lectin-induced alterations of the interaction of insulin and insulin-like growth factor 1 receptors with their ligands |
| title_short | Lectin-induced alterations of the interaction of insulin and insulin-like growth factor 1 receptors with their ligands |
| title_sort | lectin induced alterations of the interaction of insulin and insulin like growth factor 1 receptors with their ligands |
| topic | IGF1R IGF2R IR WGA Con A PHA |
| url | http://www.shd.org.yu/JSCS/Vol73/No8-9/JSCS-3762.pdf |
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