Constructing the maize inflorescence regulatory network by using efficient tsCUT&Tag assay
Maize ear development determines the crop yield, and many important transcription factors (TFs) have been identified to function in this process. However, their transcriptional regulation mechanisms are still elusive. In this study, we generated the genome-wide DNA binding sites for 8 TFs which are...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2023-06-01
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| Series: | Crop Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214514122002550 |
| _version_ | 1797807241592569856 |
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| author | Liang Dong Yanni Shi Pan Li Silin Zhong Yonghao Sun Fang Yang |
| author_facet | Liang Dong Yanni Shi Pan Li Silin Zhong Yonghao Sun Fang Yang |
| author_sort | Liang Dong |
| collection | DOAJ |
| description | Maize ear development determines the crop yield, and many important transcription factors (TFs) have been identified to function in this process. However, their transcriptional regulation mechanisms are still elusive. In this study, we generated the genome-wide DNA binding sites for 8 TFs which are known to function or highly expressed in inflorescence by applying the tsCUT&Tag method in maize leaf protoplast. We exposed a regulatory grid of 4 WUSCHEL-related homeobox (WOX) genes and revealed their potential regulatory mechanisms. In addition, a hierarchical regulation network for the determinacy and specification of maize inflorescence meristems were also constructed using the binding profiles of a floral development gene INDETERMINATE FLORAL APEX1 (IFA1) and 3 MADS-box genes. Our study provides an in-depth understanding and new insights of the regulatory mechanisms during maize inflorescence development. |
| first_indexed | 2024-03-13T06:19:36Z |
| format | Article |
| id | doaj.art-5739d40d5ecb4566b136ff8038440a26 |
| institution | Directory Open Access Journal |
| issn | 2214-5141 |
| language | English |
| last_indexed | 2024-03-13T06:19:36Z |
| publishDate | 2023-06-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Crop Journal |
| spelling | doaj.art-5739d40d5ecb4566b136ff8038440a262023-06-10T04:27:31ZengKeAi Communications Co., Ltd.Crop Journal2214-51412023-06-01113951956Constructing the maize inflorescence regulatory network by using efficient tsCUT&Tag assayLiang Dong0Yanni Shi1Pan Li2Silin Zhong3Yonghao Sun4Fang Yang5National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, Hubei, ChinaNational Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, Hubei, ChinaNational Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, Hubei, ChinaState Key Laboratory of Agrobiotechnology, School of Life Sciences, The Chinese University of Hong Kong, Shatin 999077, Hong Kong, ChinaNational Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, Hubei, China; Corresponding authors.National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, Hubei, China; Corresponding authors.Maize ear development determines the crop yield, and many important transcription factors (TFs) have been identified to function in this process. However, their transcriptional regulation mechanisms are still elusive. In this study, we generated the genome-wide DNA binding sites for 8 TFs which are known to function or highly expressed in inflorescence by applying the tsCUT&Tag method in maize leaf protoplast. We exposed a regulatory grid of 4 WUSCHEL-related homeobox (WOX) genes and revealed their potential regulatory mechanisms. In addition, a hierarchical regulation network for the determinacy and specification of maize inflorescence meristems were also constructed using the binding profiles of a floral development gene INDETERMINATE FLORAL APEX1 (IFA1) and 3 MADS-box genes. Our study provides an in-depth understanding and new insights of the regulatory mechanisms during maize inflorescence development.http://www.sciencedirect.com/science/article/pii/S2214514122002550Maize inflorescenceCUT&TagTranscription factorWOXMADS-box |
| spellingShingle | Liang Dong Yanni Shi Pan Li Silin Zhong Yonghao Sun Fang Yang Constructing the maize inflorescence regulatory network by using efficient tsCUT&Tag assay Crop Journal Maize inflorescence CUT&Tag Transcription factor WOX MADS-box |
| title | Constructing the maize inflorescence regulatory network by using efficient tsCUT&Tag assay |
| title_full | Constructing the maize inflorescence regulatory network by using efficient tsCUT&Tag assay |
| title_fullStr | Constructing the maize inflorescence regulatory network by using efficient tsCUT&Tag assay |
| title_full_unstemmed | Constructing the maize inflorescence regulatory network by using efficient tsCUT&Tag assay |
| title_short | Constructing the maize inflorescence regulatory network by using efficient tsCUT&Tag assay |
| title_sort | constructing the maize inflorescence regulatory network by using efficient tscut tag assay |
| topic | Maize inflorescence CUT&Tag Transcription factor WOX MADS-box |
| url | http://www.sciencedirect.com/science/article/pii/S2214514122002550 |
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