Comparative transcriptome analysis reveals the molecular mechanism of sterility induced by irradiation of Plutella xylostella (Linnaeus)
Plutella xylostella (Linnaeus) is an important pest of cruciferous plants, which is harmful all over the world, causing serious economic losses, and its drug resistance is increasing rapidly. The sterile insect technique (SIT) is a green control method and does not cause resistance. In this study, t...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-01-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651323013945 |
| _version_ | 1827377913288196096 |
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| author | Shifan Li Yuhang Yang Jiaqi Wen Min He Qiongbo Hu Ke Zhang Qunfang Weng |
| author_facet | Shifan Li Yuhang Yang Jiaqi Wen Min He Qiongbo Hu Ke Zhang Qunfang Weng |
| author_sort | Shifan Li |
| collection | DOAJ |
| description | Plutella xylostella (Linnaeus) is an important pest of cruciferous plants, which is harmful all over the world, causing serious economic losses, and its drug resistance is increasing rapidly. The sterile insect technique (SIT) is a green control method and does not cause resistance. In this study, transcriptomics and bioinformatics were used to explore the effects of irradiation on the reproductive function of Plutella xylostella, and the response mechanism of sterility under irradiation was initially revealed. We identified 3342 (1682 up-regulated, 1660 down-regulated), 1963 (1042 up-regulated, 921 down-regulated) and 1531 (721 up-regulated, 810 down-regulated) differentially expressed genes (DEGs) in the 200 Gy vs CK (Control Check), 400 Gy vs CK and 400 Gy vs 200 Gy groups, respectively. GO and KEGG analyses were performed for DEGs in each group. The results showed that 200 Gy activated the downstream phosphorylation pathway and inhibited the cytochrome p450 immune response mechanism. 400 Gy promoted protein decomposition and absorption pathways, autophagy pathways, etc. Down-regulated genes were concentrated in the transformation process of energy metabolizing substances such as ATP, phosphorylation signaling pathway, and insulin, while up-regulated genes were concentrated in biological regulation and metabolic processes. Eight genes in the phosphorylation pathway were selected for qRT-PCR verification, and the results showed that the phosphorylation of different dose groups was regulated in different ways. 400 Gy used positive feedback regulation, while the phosphorylation of F1 used negative feedback regulation. |
| first_indexed | 2024-03-08T12:47:17Z |
| format | Article |
| id | doaj.art-573c3c735b2b454aa6561324e4548332 |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-03-08T12:47:17Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-573c3c735b2b454aa6561324e45483322024-01-21T05:05:23ZengElsevierEcotoxicology and Environmental Safety0147-65132024-01-01270115890Comparative transcriptome analysis reveals the molecular mechanism of sterility induced by irradiation of Plutella xylostella (Linnaeus)Shifan Li0Yuhang Yang1Jiaqi Wen2Min He3Qiongbo Hu4Ke Zhang5Qunfang Weng6College of Plant Protection, South China Agricultural University, Guangzhou 510642, ChinaCollege of Plant Protection, South China Agricultural University, Guangzhou 510642, China; Dazhou Vocational and Technical College, Sichuan Province, ChinaCollege of Plant Protection, South China Agricultural University, Guangzhou 510642, ChinaCollege of Plant Protection, South China Agricultural University, Guangzhou 510642, ChinaCollege of Plant Protection, South China Agricultural University, Guangzhou 510642, China; Key Laboratory of Bio-Pesticide Innovation and Application of Guangdong Province, ChinaCollege of Plant Protection, South China Agricultural University, Guangzhou 510642, ChinaCollege of Plant Protection, South China Agricultural University, Guangzhou 510642, China; Key Laboratory of Bio-Pesticide Innovation and Application of Guangdong Province, China; Corresponding author at: College of Plant Protection, South China Agricultural University, Guangzhou 510642, China.Plutella xylostella (Linnaeus) is an important pest of cruciferous plants, which is harmful all over the world, causing serious economic losses, and its drug resistance is increasing rapidly. The sterile insect technique (SIT) is a green control method and does not cause resistance. In this study, transcriptomics and bioinformatics were used to explore the effects of irradiation on the reproductive function of Plutella xylostella, and the response mechanism of sterility under irradiation was initially revealed. We identified 3342 (1682 up-regulated, 1660 down-regulated), 1963 (1042 up-regulated, 921 down-regulated) and 1531 (721 up-regulated, 810 down-regulated) differentially expressed genes (DEGs) in the 200 Gy vs CK (Control Check), 400 Gy vs CK and 400 Gy vs 200 Gy groups, respectively. GO and KEGG analyses were performed for DEGs in each group. The results showed that 200 Gy activated the downstream phosphorylation pathway and inhibited the cytochrome p450 immune response mechanism. 400 Gy promoted protein decomposition and absorption pathways, autophagy pathways, etc. Down-regulated genes were concentrated in the transformation process of energy metabolizing substances such as ATP, phosphorylation signaling pathway, and insulin, while up-regulated genes were concentrated in biological regulation and metabolic processes. Eight genes in the phosphorylation pathway were selected for qRT-PCR verification, and the results showed that the phosphorylation of different dose groups was regulated in different ways. 400 Gy used positive feedback regulation, while the phosphorylation of F1 used negative feedback regulation.http://www.sciencedirect.com/science/article/pii/S0147651323013945Plutella xylostellaSterile insect technique (SIT)TranscriptomicsDifferentially expressed genes (DEGs)QRT-PCR |
| spellingShingle | Shifan Li Yuhang Yang Jiaqi Wen Min He Qiongbo Hu Ke Zhang Qunfang Weng Comparative transcriptome analysis reveals the molecular mechanism of sterility induced by irradiation of Plutella xylostella (Linnaeus) Ecotoxicology and Environmental Safety Plutella xylostella Sterile insect technique (SIT) Transcriptomics Differentially expressed genes (DEGs) QRT-PCR |
| title | Comparative transcriptome analysis reveals the molecular mechanism of sterility induced by irradiation of Plutella xylostella (Linnaeus) |
| title_full | Comparative transcriptome analysis reveals the molecular mechanism of sterility induced by irradiation of Plutella xylostella (Linnaeus) |
| title_fullStr | Comparative transcriptome analysis reveals the molecular mechanism of sterility induced by irradiation of Plutella xylostella (Linnaeus) |
| title_full_unstemmed | Comparative transcriptome analysis reveals the molecular mechanism of sterility induced by irradiation of Plutella xylostella (Linnaeus) |
| title_short | Comparative transcriptome analysis reveals the molecular mechanism of sterility induced by irradiation of Plutella xylostella (Linnaeus) |
| title_sort | comparative transcriptome analysis reveals the molecular mechanism of sterility induced by irradiation of plutella xylostella linnaeus |
| topic | Plutella xylostella Sterile insect technique (SIT) Transcriptomics Differentially expressed genes (DEGs) QRT-PCR |
| url | http://www.sciencedirect.com/science/article/pii/S0147651323013945 |
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