Global Gene Expression Analysis Reveals Crosstalk between Response Mechanisms to Cold and Drought Stresses in Cassava Seedlings
Abiotic stress negatively impacts cassava (Manihot esculenta) growth and yield. Several molecular mechanisms of plant response to cold and drought have been identified and described in the literature, however, little is known about the crosstalk of the responses of cassava to these two stresses. To...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2017-07-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2017.01259/full |
| _version_ | 1818527767869784064 |
|---|---|
| author | Shuxia Li Xiang Yu Zhihao Cheng Xiaoling Yu Mengbin Ruan Wenbin Li Ming Peng |
| author_facet | Shuxia Li Xiang Yu Zhihao Cheng Xiaoling Yu Mengbin Ruan Wenbin Li Ming Peng |
| author_sort | Shuxia Li |
| collection | DOAJ |
| description | Abiotic stress negatively impacts cassava (Manihot esculenta) growth and yield. Several molecular mechanisms of plant response to cold and drought have been identified and described in the literature, however, little is known about the crosstalk of the responses of cassava to these two stresses. To elucidate this question, transcriptome analysis of cassava seedlings under cold or PEG-simulated drought stress treatment was performed. Our results showed that 6103 and 7462 transcripts were significantly regulated by cold and drought stress, respectively. Gene Ontology annotation revealed that the abscisic and jasmonic acid signaling pathways shared between the two stresses responses. We further identified 2434 common differentially expressed genes (DEGs), including 1130 up-regulated and 841 down-regulated DEGs by the two stresses. These co-induced or co-suppressed genes are grouped as stress signal perception and transduction, transcription factors (TFs), metabolism as well as transport facilitation according to the function annotation. Furthermore, a large proportion of well characterized protein kinases, TF families and ubiquitin proteasome system related genes, such as RLKs, MAPKs, AP2/ERFBPs, WRKYs, MYBs, E2 enzymes and E3 ligases, including three complexes of interacting proteins were shown as key points of crosstalk between cold and drought stress signaling transduction pathways in a hierarchical manner. Our research provides valuable information and new insights for genetically improving the tolerance of crops to multiple abiotic stresses. |
| first_indexed | 2024-12-11T06:40:39Z |
| format | Article |
| id | doaj.art-573c42932ad04d9bbfd97770298255f0 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-11T06:40:39Z |
| publishDate | 2017-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-573c42932ad04d9bbfd97770298255f02022-12-22T01:17:15ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2017-07-01810.3389/fpls.2017.01259260596Global Gene Expression Analysis Reveals Crosstalk between Response Mechanisms to Cold and Drought Stresses in Cassava SeedlingsShuxia Li0Xiang Yu1Zhihao Cheng2Xiaoling Yu3Mengbin Ruan4Wenbin Li5Ming Peng6Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural SciencesHaikou, ChinaNational Key Laboratory of Plant Molecular Genetics and National Center of Plant Gene Research, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of SciencesShanghai, ChinaHaikou Experimental Station, Chinese Academy of Tropical Agricultural SciencesHaikou, ChinaInstitute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural SciencesHaikou, ChinaInstitute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural SciencesHaikou, ChinaInstitute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural SciencesHaikou, ChinaInstitute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural SciencesHaikou, ChinaAbiotic stress negatively impacts cassava (Manihot esculenta) growth and yield. Several molecular mechanisms of plant response to cold and drought have been identified and described in the literature, however, little is known about the crosstalk of the responses of cassava to these two stresses. To elucidate this question, transcriptome analysis of cassava seedlings under cold or PEG-simulated drought stress treatment was performed. Our results showed that 6103 and 7462 transcripts were significantly regulated by cold and drought stress, respectively. Gene Ontology annotation revealed that the abscisic and jasmonic acid signaling pathways shared between the two stresses responses. We further identified 2434 common differentially expressed genes (DEGs), including 1130 up-regulated and 841 down-regulated DEGs by the two stresses. These co-induced or co-suppressed genes are grouped as stress signal perception and transduction, transcription factors (TFs), metabolism as well as transport facilitation according to the function annotation. Furthermore, a large proportion of well characterized protein kinases, TF families and ubiquitin proteasome system related genes, such as RLKs, MAPKs, AP2/ERFBPs, WRKYs, MYBs, E2 enzymes and E3 ligases, including three complexes of interacting proteins were shown as key points of crosstalk between cold and drought stress signaling transduction pathways in a hierarchical manner. Our research provides valuable information and new insights for genetically improving the tolerance of crops to multiple abiotic stresses.http://journal.frontiersin.org/article/10.3389/fpls.2017.01259/fullcassavacold stressdrought stressRNA sequencing and transcriptome analysistranscription factorsprotein kinases |
| spellingShingle | Shuxia Li Xiang Yu Zhihao Cheng Xiaoling Yu Mengbin Ruan Wenbin Li Ming Peng Global Gene Expression Analysis Reveals Crosstalk between Response Mechanisms to Cold and Drought Stresses in Cassava Seedlings Frontiers in Plant Science cassava cold stress drought stress RNA sequencing and transcriptome analysis transcription factors protein kinases |
| title | Global Gene Expression Analysis Reveals Crosstalk between Response Mechanisms to Cold and Drought Stresses in Cassava Seedlings |
| title_full | Global Gene Expression Analysis Reveals Crosstalk between Response Mechanisms to Cold and Drought Stresses in Cassava Seedlings |
| title_fullStr | Global Gene Expression Analysis Reveals Crosstalk between Response Mechanisms to Cold and Drought Stresses in Cassava Seedlings |
| title_full_unstemmed | Global Gene Expression Analysis Reveals Crosstalk between Response Mechanisms to Cold and Drought Stresses in Cassava Seedlings |
| title_short | Global Gene Expression Analysis Reveals Crosstalk between Response Mechanisms to Cold and Drought Stresses in Cassava Seedlings |
| title_sort | global gene expression analysis reveals crosstalk between response mechanisms to cold and drought stresses in cassava seedlings |
| topic | cassava cold stress drought stress RNA sequencing and transcriptome analysis transcription factors protein kinases |
| url | http://journal.frontiersin.org/article/10.3389/fpls.2017.01259/full |
| work_keys_str_mv | AT shuxiali globalgeneexpressionanalysisrevealscrosstalkbetweenresponsemechanismstocoldanddroughtstressesincassavaseedlings AT xiangyu globalgeneexpressionanalysisrevealscrosstalkbetweenresponsemechanismstocoldanddroughtstressesincassavaseedlings AT zhihaocheng globalgeneexpressionanalysisrevealscrosstalkbetweenresponsemechanismstocoldanddroughtstressesincassavaseedlings AT xiaolingyu globalgeneexpressionanalysisrevealscrosstalkbetweenresponsemechanismstocoldanddroughtstressesincassavaseedlings AT mengbinruan globalgeneexpressionanalysisrevealscrosstalkbetweenresponsemechanismstocoldanddroughtstressesincassavaseedlings AT wenbinli globalgeneexpressionanalysisrevealscrosstalkbetweenresponsemechanismstocoldanddroughtstressesincassavaseedlings AT mingpeng globalgeneexpressionanalysisrevealscrosstalkbetweenresponsemechanismstocoldanddroughtstressesincassavaseedlings |