Influences of <em>Dryopteris crassirhizoma</em> Extract on<em> </em>the Viability, Growth and Virulence Properties of <em>Streptococcus </em><em>m</em><em>utans</em><em></em>
<em>Dryopteris crassirhizoma </em>is traditionally used as an herbal remedy for various diseases, and has been identified in a previous study as a potential anti-caries agent. In this study, the effect of a methanol extract of <em>D. crassirhizoma</em...
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| Format: | Article |
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MDPI AG
2012-08-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/17/8/9231 |
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| author | Suk-Ho Ban Jeong-Eun Kim Santosh Pandit Jae-Gyu Jeon |
| author_facet | Suk-Ho Ban Jeong-Eun Kim Santosh Pandit Jae-Gyu Jeon |
| author_sort | Suk-Ho Ban |
| collection | DOAJ |
| description | <em>Dryopteris crassirhizoma </em>is traditionally used as an herbal remedy for various diseases, and has been identified in a previous study as a potential anti-caries agent. In this study, the effect of a methanol extract of <em>D. crassirhizoma</em> on the viability, growth and virulence properties of <em>Str</em><em>eptococcus mutans</em>, a cariogenic dental pathogen, was investigated. In addition, the phytochemical composition of the extract was analyzed. The extract showed bactericidal and bacteriostatic activity against oral bacteria (MIC and MBC of <em>S. mutans</em>: 62.5 and 250 μg/mL, respectively). At two times the MBC, the extract significantly eliminated <em>S. mutans</em> up to 99.9% after 1 h incubation. The extract also dose-dependently reduced growth rates of <em>S. mutans</em> at sub-MIC levels. Furthermore, at sub-MIC levels, virulence properties (acid production, acid tolerance, glucosyltransferase activity and sucrose-dependent adherence) of <em>S. mutans</em> were also inhibited in a dose-dependent manner. GC-MS analysis revealed the presence of mono and disaccharides (44.9%), fatty acids (12.3%) and sugar alcohols (6.8%) in the extract. These data indicate that the extract might be useful for the control of dental caries. |
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| format | Article |
| id | doaj.art-573cd68acd6b4256be2ac1d1722fe1c7 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-23T21:04:50Z |
| publishDate | 2012-08-01 |
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| series | Molecules |
| spelling | doaj.art-573cd68acd6b4256be2ac1d1722fe1c72022-12-21T17:31:15ZengMDPI AGMolecules1420-30492012-08-011789231924410.3390/molecules17089231Influences of <em>Dryopteris crassirhizoma</em> Extract on<em> </em>the Viability, Growth and Virulence Properties of <em>Streptococcus </em><em>m</em><em>utans</em><em></em>Suk-Ho BanJeong-Eun KimSantosh PanditJae-Gyu Jeon<em>Dryopteris crassirhizoma </em>is traditionally used as an herbal remedy for various diseases, and has been identified in a previous study as a potential anti-caries agent. In this study, the effect of a methanol extract of <em>D. crassirhizoma</em> on the viability, growth and virulence properties of <em>Str</em><em>eptococcus mutans</em>, a cariogenic dental pathogen, was investigated. In addition, the phytochemical composition of the extract was analyzed. The extract showed bactericidal and bacteriostatic activity against oral bacteria (MIC and MBC of <em>S. mutans</em>: 62.5 and 250 μg/mL, respectively). At two times the MBC, the extract significantly eliminated <em>S. mutans</em> up to 99.9% after 1 h incubation. The extract also dose-dependently reduced growth rates of <em>S. mutans</em> at sub-MIC levels. Furthermore, at sub-MIC levels, virulence properties (acid production, acid tolerance, glucosyltransferase activity and sucrose-dependent adherence) of <em>S. mutans</em> were also inhibited in a dose-dependent manner. GC-MS analysis revealed the presence of mono and disaccharides (44.9%), fatty acids (12.3%) and sugar alcohols (6.8%) in the extract. These data indicate that the extract might be useful for the control of dental caries.http://www.mdpi.com/1420-3049/17/8/9231<em>Streptococcus mutans</em><em>Dryopteris crassirhizoma</em>virulence properties |
| spellingShingle | Suk-Ho Ban Jeong-Eun Kim Santosh Pandit Jae-Gyu Jeon Influences of <em>Dryopteris crassirhizoma</em> Extract on<em> </em>the Viability, Growth and Virulence Properties of <em>Streptococcus </em><em>m</em><em>utans</em><em></em> Molecules <em>Streptococcus mutans</em> <em>Dryopteris crassirhizoma</em> virulence properties |
| title | Influences of <em>Dryopteris crassirhizoma</em> Extract on<em> </em>the Viability, Growth and Virulence Properties of <em>Streptococcus </em><em>m</em><em>utans</em><em></em> |
| title_full | Influences of <em>Dryopteris crassirhizoma</em> Extract on<em> </em>the Viability, Growth and Virulence Properties of <em>Streptococcus </em><em>m</em><em>utans</em><em></em> |
| title_fullStr | Influences of <em>Dryopteris crassirhizoma</em> Extract on<em> </em>the Viability, Growth and Virulence Properties of <em>Streptococcus </em><em>m</em><em>utans</em><em></em> |
| title_full_unstemmed | Influences of <em>Dryopteris crassirhizoma</em> Extract on<em> </em>the Viability, Growth and Virulence Properties of <em>Streptococcus </em><em>m</em><em>utans</em><em></em> |
| title_short | Influences of <em>Dryopteris crassirhizoma</em> Extract on<em> </em>the Viability, Growth and Virulence Properties of <em>Streptococcus </em><em>m</em><em>utans</em><em></em> |
| title_sort | influences of lt em gt dryopteris crassirhizoma lt em gt extract on lt em gt lt em gt the viability growth and virulence properties of lt em gt streptococcus lt em gt lt em gt m lt em gt lt em gt utans lt em gt lt em gt lt em gt |
| topic | <em>Streptococcus mutans</em> <em>Dryopteris crassirhizoma</em> virulence properties |
| url | http://www.mdpi.com/1420-3049/17/8/9231 |
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