Upregulation of LSD1 promotes migration and invasion in gastric cancer through facilitating EMT
Jin Zhang,1,* Donghui Zhao,1,* Qingjun Li,2 Xiuluan Du,1 Yanxiang Liu,1 Xin Dai,1 Lianqing Hong3 1Department of Pathology, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou, P.R. China; 2Department of Neurology, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou,...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2019-05-01
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| Series: | Cancer Management and Research |
| Subjects: | |
| Online Access: | https://www.dovepress.com/upregulation-of-lsd1-promotes-migration-and-invasion-in-gastric-cancer-peer-reviewed-article-CMAR |
| _version_ | 1819091656981348352 |
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| author | Zhang J Zhao D Li Q Du X Liu Y Dai X Hong L |
| author_facet | Zhang J Zhao D Li Q Du X Liu Y Dai X Hong L |
| author_sort | Zhang J |
| collection | DOAJ |
| description | Jin Zhang,1,* Donghui Zhao,1,* Qingjun Li,2 Xiuluan Du,1 Yanxiang Liu,1 Xin Dai,1 Lianqing Hong3 1Department of Pathology, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou, P.R. China; 2Department of Neurology, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou, P.R. China; 3Department of Pathology, Nanjing Integrated Traditional Chinese and Western Medicine Hospital Affiliated with Nanjing University of Chinese Medicine, Nanjing, P.R. China *These authors contributed equally to this work Background: Gastric cancer (GC) is a common malignant tumor of the digestive system. In addition, GC metastasis is an extremely complicated process. A previous study has found that lysine-specific demethylase 1 (LSD1) is abnormal expression in a variety of cancers and its overexpression correlates with aggressive disease and poor outcome. Methods: qRT-PCR and Western blot assays were used to assess the expression of LSD1 in GC tissue samples and cell lines. Colony formation assay, CCK-8 assay, scratch-wound assay and transwell invasion, were performed to determine the effect of LSD1 on cell proliferation and migration as well as invasion in GC. Results: Our results show that LSD1 was up-regulated in GC tumor tissues and cell lines, and high expression level of LSD1 was found to be positively correlated with tumor size, lymph node metastasis and pathological grade. Moreover, LSD1 promoted cell proliferation, migration and invasion of GC. In addition, LSD1 regulated E-cadherin expression through demethylating H3K4me2, thereby promoting EMT in GC. Conclusion: Our work indicated that LSD1 may be used as a potential target of gastric cancer. Keywords: LSD1, EMT, E-cadherin, H3K4me2, gastric cancer |
| first_indexed | 2024-12-21T22:43:12Z |
| format | Article |
| id | doaj.art-573d22bf0cb34c159078033e4b230f79 |
| institution | Directory Open Access Journal |
| issn | 1179-1322 |
| language | English |
| last_indexed | 2024-12-21T22:43:12Z |
| publishDate | 2019-05-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Cancer Management and Research |
| spelling | doaj.art-573d22bf0cb34c159078033e4b230f792022-12-21T18:47:47ZengDove Medical PressCancer Management and Research1179-13222019-05-01Volume 114481449145839Upregulation of LSD1 promotes migration and invasion in gastric cancer through facilitating EMTZhang JZhao DLi QDu XLiu YDai XHong LJin Zhang,1,* Donghui Zhao,1,* Qingjun Li,2 Xiuluan Du,1 Yanxiang Liu,1 Xin Dai,1 Lianqing Hong3 1Department of Pathology, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou, P.R. China; 2Department of Neurology, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou, P.R. China; 3Department of Pathology, Nanjing Integrated Traditional Chinese and Western Medicine Hospital Affiliated with Nanjing University of Chinese Medicine, Nanjing, P.R. China *These authors contributed equally to this work Background: Gastric cancer (GC) is a common malignant tumor of the digestive system. In addition, GC metastasis is an extremely complicated process. A previous study has found that lysine-specific demethylase 1 (LSD1) is abnormal expression in a variety of cancers and its overexpression correlates with aggressive disease and poor outcome. Methods: qRT-PCR and Western blot assays were used to assess the expression of LSD1 in GC tissue samples and cell lines. Colony formation assay, CCK-8 assay, scratch-wound assay and transwell invasion, were performed to determine the effect of LSD1 on cell proliferation and migration as well as invasion in GC. Results: Our results show that LSD1 was up-regulated in GC tumor tissues and cell lines, and high expression level of LSD1 was found to be positively correlated with tumor size, lymph node metastasis and pathological grade. Moreover, LSD1 promoted cell proliferation, migration and invasion of GC. In addition, LSD1 regulated E-cadherin expression through demethylating H3K4me2, thereby promoting EMT in GC. Conclusion: Our work indicated that LSD1 may be used as a potential target of gastric cancer. Keywords: LSD1, EMT, E-cadherin, H3K4me2, gastric cancerhttps://www.dovepress.com/upregulation-of-lsd1-promotes-migration-and-invasion-in-gastric-cancer-peer-reviewed-article-CMARLSD1EMTE-cadherinH3K4me2gastric cancer |
| spellingShingle | Zhang J Zhao D Li Q Du X Liu Y Dai X Hong L Upregulation of LSD1 promotes migration and invasion in gastric cancer through facilitating EMT Cancer Management and Research LSD1 EMT E-cadherin H3K4me2 gastric cancer |
| title | Upregulation of LSD1 promotes migration and invasion in gastric cancer through facilitating EMT |
| title_full | Upregulation of LSD1 promotes migration and invasion in gastric cancer through facilitating EMT |
| title_fullStr | Upregulation of LSD1 promotes migration and invasion in gastric cancer through facilitating EMT |
| title_full_unstemmed | Upregulation of LSD1 promotes migration and invasion in gastric cancer through facilitating EMT |
| title_short | Upregulation of LSD1 promotes migration and invasion in gastric cancer through facilitating EMT |
| title_sort | upregulation of lsd1 promotes migration and invasion in gastric cancer through facilitating emt |
| topic | LSD1 EMT E-cadherin H3K4me2 gastric cancer |
| url | https://www.dovepress.com/upregulation-of-lsd1-promotes-migration-and-invasion-in-gastric-cancer-peer-reviewed-article-CMAR |
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