Serum Cholesterol Efflux Capacity in Age-Related Macular Degeneration and Polypoidal Choroidal Vasculopathy
Purpose: To investigate serum cholesterol efflux capacity (the ability of the serum to accept cholesterol) and factors that regulate it using nuclear magnetic resonance-quantified measures of lipoprotein particle composition and size and apolipoproteins metrics in patients with age-related macular d...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-06-01
|
| Series: | Ophthalmology Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666914522000318 |
| _version_ | 1818218852774838272 |
|---|---|
| author | Yasuo Yanagi, MD, PhD Richard M.C. Yu, PhD Waseem Ahamed, MS Marco Yu, PhD Kelvin Yi Chong Teo, MBBS, MMed (Ophth) Anna C.S. Tan, MBBS Ching-Yu Cheng, MD, PhD Tien Yin Wong, MD, PhD Rajendra S. Apte, MD, PhD Chui Ming Gemmy Cheung, FRCOphth |
| author_facet | Yasuo Yanagi, MD, PhD Richard M.C. Yu, PhD Waseem Ahamed, MS Marco Yu, PhD Kelvin Yi Chong Teo, MBBS, MMed (Ophth) Anna C.S. Tan, MBBS Ching-Yu Cheng, MD, PhD Tien Yin Wong, MD, PhD Rajendra S. Apte, MD, PhD Chui Ming Gemmy Cheung, FRCOphth |
| author_sort | Yasuo Yanagi, MD, PhD |
| collection | DOAJ |
| description | Purpose: To investigate serum cholesterol efflux capacity (the ability of the serum to accept cholesterol) and factors that regulate it using nuclear magnetic resonance-quantified measures of lipoprotein particle composition and size and apolipoproteins metrics in patients with age-related macular degeneration (AMD). Design: Case-control study. Participants: Four hundred two serum samples from 80 patients with early AMD (eAMD), and 212 patients with neovascular AMD (nAMD), including 80 with typical nAMD (tAMD) and 132 with polypoidal choroidal vasculopathy (PCV), and 110 age- and gender matched control participants. Methods: Serum from participants showed cholesterol efflux capacity measured using in vitro cell assays and lipoprotein subfractions measured using nuclear magnetic resonance (Nightingale, Ltd). Associations between cholesterol efflux capacity (measured in percentage) and lipid subfractions were investigated in the patients and control participants. Main Outcome Measures: Cholesterol efflux capacity and lipid subfractions in control, eAMD, and nAMD. Associations between HDL subfractions and cholesterol efflux capacity. Results: Cholesterol efflux capacity was higher in patients with eAMD (68.0 ± 11.3% [mean ± standard deviation]) and nAMD (75.9 ± 27.7%) than in the control participants (56.9 ± 16.7%) after adjusting for age, gender, and use of lipid-lowering drug (P < 0.0001). Nuclear magnetic resonance lipidomics demonstrated that the mean diameter of HDL was larger both in eAMD (9.96 ± 0.27 mm [mean ± standard deviation]) and PCV (9.97 ± 0.23 mm) compared with that of the control participants (9.84 ± 0.24 mm; P = 0.0001 for both). Among the 28 HDL subfractions, most of the small, medium, and large HDLs, but none of the 7 extra large HDLs fractions, were associated moderately with cholesterol efflux capacity in eAMD and PCV (R = 0.149–0.277). Conclusions: Serum cholesterol efflux capacity was increased in eAMD and PCV, but not tAMD, possibly reflecting differential underlying pathophysiologic features of lipid dysregulation in tAMD and PCV. Further studies should be directed toward investigating the diverse biological activities of HDL in AMD, including macular pigment transport, regulation of inflammation, and local cholesterol transport system. |
| first_indexed | 2024-12-12T07:30:21Z |
| format | Article |
| id | doaj.art-573ef46f93b44e8f92f24288b3c950ce |
| institution | Directory Open Access Journal |
| issn | 2666-9145 |
| language | English |
| last_indexed | 2024-12-12T07:30:21Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ophthalmology Science |
| spelling | doaj.art-573ef46f93b44e8f92f24288b3c950ce2022-12-22T00:33:02ZengElsevierOphthalmology Science2666-91452022-06-0122100142Serum Cholesterol Efflux Capacity in Age-Related Macular Degeneration and Polypoidal Choroidal VasculopathyYasuo Yanagi, MD, PhD0Richard M.C. Yu, PhD1Waseem Ahamed, MS2Marco Yu, PhD3Kelvin Yi Chong Teo, MBBS, MMed (Ophth)4Anna C.S. Tan, MBBS5Ching-Yu Cheng, MD, PhD6Tien Yin Wong, MD, PhD7Rajendra S. Apte, MD, PhD8Chui Ming Gemmy Cheung, FRCOphth9Singapore National Eye Centre, Singapore Eye Research Institute, Singapore, Republic of Singapore; Academic Clinical Program, Duke-NUS Medical School, National University of Singapore, Singapore, Republic of Singapore; Correspondence: Yasuo Yanagi, Singapore Eye Research Institute, 11 Third Hospital Avenue, 168751, Singapore, Republic of Singapore.Singapore National Eye Centre, Singapore Eye Research Institute, Singapore, Republic of SingaporeSingapore National Eye Centre, Singapore Eye Research Institute, Singapore, Republic of SingaporeSingapore National Eye Centre, Singapore Eye Research Institute, Singapore, Republic of Singapore; Academic Clinical Program, Duke-NUS Medical School, National University of Singapore, Singapore, Republic of SingaporeSingapore National Eye Centre, Singapore Eye Research Institute, Singapore, Republic of Singapore; Academic Clinical Program, Duke-NUS Medical School, National University of Singapore, Singapore, Republic of SingaporeSingapore National Eye Centre, Singapore Eye Research Institute, Singapore, Republic of Singapore; Academic Clinical Program, Duke-NUS Medical School, National University of Singapore, Singapore, Republic of SingaporeSingapore National Eye Centre, Singapore Eye Research Institute, Singapore, Republic of Singapore; Academic Clinical Program, Duke-NUS Medical School, National University of Singapore, Singapore, Republic of SingaporeSingapore National Eye Centre, Singapore Eye Research Institute, Singapore, Republic of Singapore; Academic Clinical Program, Duke-NUS Medical School, National University of Singapore, Singapore, Republic of SingaporeDepartment of Ophthalmology, Washington University School of Medicine, St. Louis, Missouri; Department of Medicine, Washington University School of Medicine, St. Louis, Missouri; Department of Developmental Biology, Washington University School of Medicine, St. Louis, MissouriSingapore National Eye Centre, Singapore Eye Research Institute, Singapore, Republic of Singapore; Academic Clinical Program, Duke-NUS Medical School, National University of Singapore, Singapore, Republic of SingaporePurpose: To investigate serum cholesterol efflux capacity (the ability of the serum to accept cholesterol) and factors that regulate it using nuclear magnetic resonance-quantified measures of lipoprotein particle composition and size and apolipoproteins metrics in patients with age-related macular degeneration (AMD). Design: Case-control study. Participants: Four hundred two serum samples from 80 patients with early AMD (eAMD), and 212 patients with neovascular AMD (nAMD), including 80 with typical nAMD (tAMD) and 132 with polypoidal choroidal vasculopathy (PCV), and 110 age- and gender matched control participants. Methods: Serum from participants showed cholesterol efflux capacity measured using in vitro cell assays and lipoprotein subfractions measured using nuclear magnetic resonance (Nightingale, Ltd). Associations between cholesterol efflux capacity (measured in percentage) and lipid subfractions were investigated in the patients and control participants. Main Outcome Measures: Cholesterol efflux capacity and lipid subfractions in control, eAMD, and nAMD. Associations between HDL subfractions and cholesterol efflux capacity. Results: Cholesterol efflux capacity was higher in patients with eAMD (68.0 ± 11.3% [mean ± standard deviation]) and nAMD (75.9 ± 27.7%) than in the control participants (56.9 ± 16.7%) after adjusting for age, gender, and use of lipid-lowering drug (P < 0.0001). Nuclear magnetic resonance lipidomics demonstrated that the mean diameter of HDL was larger both in eAMD (9.96 ± 0.27 mm [mean ± standard deviation]) and PCV (9.97 ± 0.23 mm) compared with that of the control participants (9.84 ± 0.24 mm; P = 0.0001 for both). Among the 28 HDL subfractions, most of the small, medium, and large HDLs, but none of the 7 extra large HDLs fractions, were associated moderately with cholesterol efflux capacity in eAMD and PCV (R = 0.149–0.277). Conclusions: Serum cholesterol efflux capacity was increased in eAMD and PCV, but not tAMD, possibly reflecting differential underlying pathophysiologic features of lipid dysregulation in tAMD and PCV. Further studies should be directed toward investigating the diverse biological activities of HDL in AMD, including macular pigment transport, regulation of inflammation, and local cholesterol transport system.http://www.sciencedirect.com/science/article/pii/S2666914522000318Age-related macular degenerationCholesterol effluxDrusenLipoproteinPolypoidal choroidal vasculopathy |
| spellingShingle | Yasuo Yanagi, MD, PhD Richard M.C. Yu, PhD Waseem Ahamed, MS Marco Yu, PhD Kelvin Yi Chong Teo, MBBS, MMed (Ophth) Anna C.S. Tan, MBBS Ching-Yu Cheng, MD, PhD Tien Yin Wong, MD, PhD Rajendra S. Apte, MD, PhD Chui Ming Gemmy Cheung, FRCOphth Serum Cholesterol Efflux Capacity in Age-Related Macular Degeneration and Polypoidal Choroidal Vasculopathy Ophthalmology Science Age-related macular degeneration Cholesterol efflux Drusen Lipoprotein Polypoidal choroidal vasculopathy |
| title | Serum Cholesterol Efflux Capacity in Age-Related Macular Degeneration and Polypoidal Choroidal Vasculopathy |
| title_full | Serum Cholesterol Efflux Capacity in Age-Related Macular Degeneration and Polypoidal Choroidal Vasculopathy |
| title_fullStr | Serum Cholesterol Efflux Capacity in Age-Related Macular Degeneration and Polypoidal Choroidal Vasculopathy |
| title_full_unstemmed | Serum Cholesterol Efflux Capacity in Age-Related Macular Degeneration and Polypoidal Choroidal Vasculopathy |
| title_short | Serum Cholesterol Efflux Capacity in Age-Related Macular Degeneration and Polypoidal Choroidal Vasculopathy |
| title_sort | serum cholesterol efflux capacity in age related macular degeneration and polypoidal choroidal vasculopathy |
| topic | Age-related macular degeneration Cholesterol efflux Drusen Lipoprotein Polypoidal choroidal vasculopathy |
| url | http://www.sciencedirect.com/science/article/pii/S2666914522000318 |
| work_keys_str_mv | AT yasuoyanagimdphd serumcholesteroleffluxcapacityinagerelatedmaculardegenerationandpolypoidalchoroidalvasculopathy AT richardmcyuphd serumcholesteroleffluxcapacityinagerelatedmaculardegenerationandpolypoidalchoroidalvasculopathy AT waseemahamedms serumcholesteroleffluxcapacityinagerelatedmaculardegenerationandpolypoidalchoroidalvasculopathy AT marcoyuphd serumcholesteroleffluxcapacityinagerelatedmaculardegenerationandpolypoidalchoroidalvasculopathy AT kelvinyichongteombbsmmedophth serumcholesteroleffluxcapacityinagerelatedmaculardegenerationandpolypoidalchoroidalvasculopathy AT annacstanmbbs serumcholesteroleffluxcapacityinagerelatedmaculardegenerationandpolypoidalchoroidalvasculopathy AT chingyuchengmdphd serumcholesteroleffluxcapacityinagerelatedmaculardegenerationandpolypoidalchoroidalvasculopathy AT tienyinwongmdphd serumcholesteroleffluxcapacityinagerelatedmaculardegenerationandpolypoidalchoroidalvasculopathy AT rajendrasaptemdphd serumcholesteroleffluxcapacityinagerelatedmaculardegenerationandpolypoidalchoroidalvasculopathy AT chuiminggemmycheungfrcophth serumcholesteroleffluxcapacityinagerelatedmaculardegenerationandpolypoidalchoroidalvasculopathy |