Characterization of <i>Salinivibrio socompensis</i> sp. nov., A New Halophilic Bacterium Isolated from the High-Altitude Hypersaline Lake Socompa, Argentina
The genus <i>Salinivibrio</i> belongs to the family <i>Vibrionaceae</i> and includes Gram-stain-negative, motile by a polar flagellum, and facultatively anaerobic curved rods. They are halophilic bacteria commonly found in hypersaline aquatic habitats and salted foods. This g...
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MDPI AG
2019-08-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/7/8/241 |
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| author | Cristina Galisteo Cristina Sánchez-Porro Rafael R. de la Haba Clara López-Hermoso Ana B. Fernández María E. Farias Antonio Ventosa |
| author_facet | Cristina Galisteo Cristina Sánchez-Porro Rafael R. de la Haba Clara López-Hermoso Ana B. Fernández María E. Farias Antonio Ventosa |
| author_sort | Cristina Galisteo |
| collection | DOAJ |
| description | The genus <i>Salinivibrio</i> belongs to the family <i>Vibrionaceae</i> and includes Gram-stain-negative, motile by a polar flagellum, and facultatively anaerobic curved rods. They are halophilic bacteria commonly found in hypersaline aquatic habitats and salted foods. This genus includes five species and two subspecies. A presumed novel species, strain S35<sup>T</sup>, was previously isolated from the high-altitude volcanic, alkaline, and saline lake Socompa (Argentinean Andes). In this study we carried out a complete taxonomic characterization of strain S35<sup>T</sup>, including the 16S rRNA gene sequence and core-genome analysis, the average nucleotide identity (ANIb, ANIm, and orthoANI), and <i>in silico</i> DNA−DNA hybridization (GGDC), as well as the phenotypic and chemotaxonomic characterization. It grew at 3%−20% (w/v) NaCl, pH 6−10, and 10−42 °C, with optimum growth at 7.0%−7.5% (w/v) NaCl, pH 8.0, and 37 °C, respectively. Strain S35<sup>T</sup> was oxidase- and catalase-positive, able to produce acid from D-glucose and other carbohydrates. Hydrolysis of DNA, methyl red test, and nitrate and nitrite reduction were positive. Its main fatty acids were C<sub>16:0</sub>, C<sub>16:1</sub> <i>ω</i>7<i>c</i> and C<sub>16:1</sub> <i>ω</i>6<i>c</i>, and C<sub>18:1</sub> <i>ω</i>7<i>c</i> and/or C<sub>18:1</sub> <i>ω</i>6<i>c</i>. ANI, GGDC, and core-genome analysis determined that strain S35<sup>T</sup> constitutes a novel species of the genus <i>Salinivibrio</i>, for which the name <i>Salinivibrio</i> <i>socompensis</i> sp. nov. is proposed. The type strain is S35<sup>T</sup> (= CECT 9634<sup>T</sup> = BNM 0535<sup>T</sup>). |
| first_indexed | 2024-12-10T19:23:08Z |
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| institution | Directory Open Access Journal |
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| last_indexed | 2024-12-10T19:23:08Z |
| publishDate | 2019-08-01 |
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| spelling | doaj.art-573f6ab1a6594ca6a2486a87a73d5dcf2022-12-22T01:36:26ZengMDPI AGMicroorganisms2076-26072019-08-017824110.3390/microorganisms7080241microorganisms7080241Characterization of <i>Salinivibrio socompensis</i> sp. nov., A New Halophilic Bacterium Isolated from the High-Altitude Hypersaline Lake Socompa, ArgentinaCristina Galisteo0Cristina Sánchez-Porro1Rafael R. de la Haba2Clara López-Hermoso3Ana B. Fernández4María E. Farias5Antonio Ventosa6Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, SpainDepartment of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, SpainDepartment of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, SpainDepartment of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, SpainDepartment of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, SpainLaboratorio de Investigaciones Microbiológicas de Lagunas Andinas, Planta Piloto de Procesos Industriales Microbiológicos, Centro Científico Tecnológico, CONICET Tucumán T4000, ArgentinaDepartment of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, SpainThe genus <i>Salinivibrio</i> belongs to the family <i>Vibrionaceae</i> and includes Gram-stain-negative, motile by a polar flagellum, and facultatively anaerobic curved rods. They are halophilic bacteria commonly found in hypersaline aquatic habitats and salted foods. This genus includes five species and two subspecies. A presumed novel species, strain S35<sup>T</sup>, was previously isolated from the high-altitude volcanic, alkaline, and saline lake Socompa (Argentinean Andes). In this study we carried out a complete taxonomic characterization of strain S35<sup>T</sup>, including the 16S rRNA gene sequence and core-genome analysis, the average nucleotide identity (ANIb, ANIm, and orthoANI), and <i>in silico</i> DNA−DNA hybridization (GGDC), as well as the phenotypic and chemotaxonomic characterization. It grew at 3%−20% (w/v) NaCl, pH 6−10, and 10−42 °C, with optimum growth at 7.0%−7.5% (w/v) NaCl, pH 8.0, and 37 °C, respectively. Strain S35<sup>T</sup> was oxidase- and catalase-positive, able to produce acid from D-glucose and other carbohydrates. Hydrolysis of DNA, methyl red test, and nitrate and nitrite reduction were positive. Its main fatty acids were C<sub>16:0</sub>, C<sub>16:1</sub> <i>ω</i>7<i>c</i> and C<sub>16:1</sub> <i>ω</i>6<i>c</i>, and C<sub>18:1</sub> <i>ω</i>7<i>c</i> and/or C<sub>18:1</sub> <i>ω</i>6<i>c</i>. ANI, GGDC, and core-genome analysis determined that strain S35<sup>T</sup> constitutes a novel species of the genus <i>Salinivibrio</i>, for which the name <i>Salinivibrio</i> <i>socompensis</i> sp. nov. is proposed. The type strain is S35<sup>T</sup> (= CECT 9634<sup>T</sup> = BNM 0535<sup>T</sup>).https://www.mdpi.com/2076-2607/7/8/241<i>Salinivibrio</i><i>Salinivibrio socompensis</i>bacterial taxonomyhalophilic bacterianew specieshypersaline lake |
| spellingShingle | Cristina Galisteo Cristina Sánchez-Porro Rafael R. de la Haba Clara López-Hermoso Ana B. Fernández María E. Farias Antonio Ventosa Characterization of <i>Salinivibrio socompensis</i> sp. nov., A New Halophilic Bacterium Isolated from the High-Altitude Hypersaline Lake Socompa, Argentina Microorganisms <i>Salinivibrio</i> <i>Salinivibrio socompensis</i> bacterial taxonomy halophilic bacteria new species hypersaline lake |
| title | Characterization of <i>Salinivibrio socompensis</i> sp. nov., A New Halophilic Bacterium Isolated from the High-Altitude Hypersaline Lake Socompa, Argentina |
| title_full | Characterization of <i>Salinivibrio socompensis</i> sp. nov., A New Halophilic Bacterium Isolated from the High-Altitude Hypersaline Lake Socompa, Argentina |
| title_fullStr | Characterization of <i>Salinivibrio socompensis</i> sp. nov., A New Halophilic Bacterium Isolated from the High-Altitude Hypersaline Lake Socompa, Argentina |
| title_full_unstemmed | Characterization of <i>Salinivibrio socompensis</i> sp. nov., A New Halophilic Bacterium Isolated from the High-Altitude Hypersaline Lake Socompa, Argentina |
| title_short | Characterization of <i>Salinivibrio socompensis</i> sp. nov., A New Halophilic Bacterium Isolated from the High-Altitude Hypersaline Lake Socompa, Argentina |
| title_sort | characterization of i salinivibrio socompensis i sp nov a new halophilic bacterium isolated from the high altitude hypersaline lake socompa argentina |
| topic | <i>Salinivibrio</i> <i>Salinivibrio socompensis</i> bacterial taxonomy halophilic bacteria new species hypersaline lake |
| url | https://www.mdpi.com/2076-2607/7/8/241 |
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