A Comparative Study of Mesenchymal Stem Cell-Derived Extracellular Vesicles’ Local and Systemic Dose-Dependent Administration in Rat Spinal Cord Injury
Spinal cord injury (SCI) is a serious neurological condition that causes severe disability. One of the approaches to overcoming the complications of SCI is stem cell-derived extracellular vesicle (EV) therapy. In this research, we performed a comparative evaluation of rat spinal cord post-traumatic...
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2022-12-01
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| Online Access: | https://www.mdpi.com/2079-7737/11/12/1853 |
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| author | Alexander Kostennikov Ilyas Kabdesh Davran Sabirov Anna Timofeeva Alexander Rogozhin Ilya Shulman Albert Rizvanov Yana Mukhamedshina |
| author_facet | Alexander Kostennikov Ilyas Kabdesh Davran Sabirov Anna Timofeeva Alexander Rogozhin Ilya Shulman Albert Rizvanov Yana Mukhamedshina |
| author_sort | Alexander Kostennikov |
| collection | DOAJ |
| description | Spinal cord injury (SCI) is a serious neurological condition that causes severe disability. One of the approaches to overcoming the complications of SCI is stem cell-derived extracellular vesicle (EV) therapy. In this research, we performed a comparative evaluation of rat spinal cord post-traumatic regeneration efficacy using different methods of mesenchymal stem cell-derived EV transplantation (local vs. systemic) followed by evaluation of their minimal therapeutic dose. The results suggested that MSC-EV therapy could improve locomotor activity over 60 days after the SCI, showing a dose-dependent effect on the recovery of spinal cord motor pathways. We also established the possibility of maintaining a population of mature oligodendrocytes by MSC-EVs. It was observed that in the spinal cord injury area, intravenous transplantation of MSC-EVs showed more pronounced therapeutic effects compared to the treatment of fibrin matrix-encapsulated MSC-EVs. |
| first_indexed | 2024-03-09T17:18:09Z |
| format | Article |
| id | doaj.art-5743cccc9bd54246833b618780ebf74e |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-09T17:18:09Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-5743cccc9bd54246833b618780ebf74e2023-11-24T13:24:40ZengMDPI AGBiology2079-77372022-12-011112185310.3390/biology11121853A Comparative Study of Mesenchymal Stem Cell-Derived Extracellular Vesicles’ Local and Systemic Dose-Dependent Administration in Rat Spinal Cord InjuryAlexander Kostennikov0Ilyas Kabdesh1Davran Sabirov2Anna Timofeeva3Alexander Rogozhin4Ilya Shulman5Albert Rizvanov6Yana Mukhamedshina7Center for Clinical Research for Precision and Regenerative Medicine, Institute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, RussiaCenter for Clinical Research for Precision and Regenerative Medicine, Institute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, RussiaCenter for Clinical Research for Precision and Regenerative Medicine, Institute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, RussiaCenter for Clinical Research for Precision and Regenerative Medicine, Institute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, RussiaCenter for Clinical Research for Precision and Regenerative Medicine, Institute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, RussiaNeurosurgical Department No. 2, Republic Clinical Hospital, 420138 Kazan, RussiaCenter for Clinical Research for Precision and Regenerative Medicine, Institute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, RussiaCenter for Clinical Research for Precision and Regenerative Medicine, Institute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, RussiaSpinal cord injury (SCI) is a serious neurological condition that causes severe disability. One of the approaches to overcoming the complications of SCI is stem cell-derived extracellular vesicle (EV) therapy. In this research, we performed a comparative evaluation of rat spinal cord post-traumatic regeneration efficacy using different methods of mesenchymal stem cell-derived EV transplantation (local vs. systemic) followed by evaluation of their minimal therapeutic dose. The results suggested that MSC-EV therapy could improve locomotor activity over 60 days after the SCI, showing a dose-dependent effect on the recovery of spinal cord motor pathways. We also established the possibility of maintaining a population of mature oligodendrocytes by MSC-EVs. It was observed that in the spinal cord injury area, intravenous transplantation of MSC-EVs showed more pronounced therapeutic effects compared to the treatment of fibrin matrix-encapsulated MSC-EVs.https://www.mdpi.com/2079-7737/11/12/1853spinal cord injurymesenchymal stem cellsextracellular vesiclessystemic administrationlocal administrationrats |
| spellingShingle | Alexander Kostennikov Ilyas Kabdesh Davran Sabirov Anna Timofeeva Alexander Rogozhin Ilya Shulman Albert Rizvanov Yana Mukhamedshina A Comparative Study of Mesenchymal Stem Cell-Derived Extracellular Vesicles’ Local and Systemic Dose-Dependent Administration in Rat Spinal Cord Injury Biology spinal cord injury mesenchymal stem cells extracellular vesicles systemic administration local administration rats |
| title | A Comparative Study of Mesenchymal Stem Cell-Derived Extracellular Vesicles’ Local and Systemic Dose-Dependent Administration in Rat Spinal Cord Injury |
| title_full | A Comparative Study of Mesenchymal Stem Cell-Derived Extracellular Vesicles’ Local and Systemic Dose-Dependent Administration in Rat Spinal Cord Injury |
| title_fullStr | A Comparative Study of Mesenchymal Stem Cell-Derived Extracellular Vesicles’ Local and Systemic Dose-Dependent Administration in Rat Spinal Cord Injury |
| title_full_unstemmed | A Comparative Study of Mesenchymal Stem Cell-Derived Extracellular Vesicles’ Local and Systemic Dose-Dependent Administration in Rat Spinal Cord Injury |
| title_short | A Comparative Study of Mesenchymal Stem Cell-Derived Extracellular Vesicles’ Local and Systemic Dose-Dependent Administration in Rat Spinal Cord Injury |
| title_sort | comparative study of mesenchymal stem cell derived extracellular vesicles local and systemic dose dependent administration in rat spinal cord injury |
| topic | spinal cord injury mesenchymal stem cells extracellular vesicles systemic administration local administration rats |
| url | https://www.mdpi.com/2079-7737/11/12/1853 |
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