Analysis of Phytoconstituents and Biological Activities of Different Parts of Mahonia nepalensis and Berberis aristata
The phytochemicals and biological activities of extracts from leaves and stem of Mahonia nepalensis and Berberis aristata were carried out. Phytochemical screening showed the presence of alkaloids, steroids, polyphenols, quinones, glycoside, flavonoid, terpenoid and cardiac glycoside in the hexane,...
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| Format: | Article |
| Language: | English |
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Biotechnology Society of Nepal
2017-12-01
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| Series: | Nepal Journal of Biotechnology |
| Subjects: |
| _version_ | 1818864224251674624 |
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| author | Rojeena Thusa Sushika Mulmi |
| author_facet | Rojeena Thusa Sushika Mulmi |
| author_sort | Rojeena Thusa |
| collection | DOAJ |
| description | The phytochemicals and biological activities of extracts from leaves and stem of Mahonia nepalensis and Berberis aristata were carried out. Phytochemical screening showed the presence of alkaloids, steroids, polyphenols, quinones, glycoside, flavonoid, terpenoid and cardiac glycoside in the hexane, ethyl acetate and methanol extracts of leaf and stem of these two plants. The column chromatography of methanol extract of stem of Mahonia nepalensis resulted in isolation of four pure compounds MN1, MN2, MN3 and MN4. Out of four isolated compounds, two were identified as MN1:b- sitosterol and MN2: Berberine by comparison of melting point, Co-TLC, IR and UV spectra of authentic sample. Potent pharmacological activity of Mahonia nepalensis and Berberis aristata were revealed from antimicrobial activity and brine shrimp bioassay. Methanol extracts of stem of Mahonia nepalensis and Berberis aristata showed significant zone of inhibition of 18 mm and 21 mm respectively against the Staphylococcus aureus. Methanol extract of Berberis aristata were comparatively little stronger against Staphylococcus aureus than methanol extract of Mahonia nepalensis. LC50 values (μg/ml) of methanol extracts of stem of Berberis aristata and Mahonia nepalensis were found to be 8.058x10-4 and 8.3 whereas methanol extracts of leaf of Mahonia nepalensis and Berberis aristata were 389.04 and 1303.166 respectively. |
| first_indexed | 2024-12-19T10:28:15Z |
| format | Article |
| id | doaj.art-574b98fbd91c4b89baf2461765f3420b |
| institution | Directory Open Access Journal |
| issn | 2091-1130 2467-9313 |
| language | English |
| last_indexed | 2024-12-19T10:28:15Z |
| publishDate | 2017-12-01 |
| publisher | Biotechnology Society of Nepal |
| record_format | Article |
| series | Nepal Journal of Biotechnology |
| spelling | doaj.art-574b98fbd91c4b89baf2461765f3420b2022-12-21T20:25:50ZengBiotechnology Society of NepalNepal Journal of Biotechnology2091-11302467-93132017-12-015151310.3126/njb.v5i1.18864Analysis of Phytoconstituents and Biological Activities of Different Parts of Mahonia nepalensis and Berberis aristataRojeena Thusa0Sushika Mulmi1Central Department of Chemistry, Tribhuvan University, KirtipurCentral Department of Chemistry, Tribhuvan University, KirtipurThe phytochemicals and biological activities of extracts from leaves and stem of Mahonia nepalensis and Berberis aristata were carried out. Phytochemical screening showed the presence of alkaloids, steroids, polyphenols, quinones, glycoside, flavonoid, terpenoid and cardiac glycoside in the hexane, ethyl acetate and methanol extracts of leaf and stem of these two plants. The column chromatography of methanol extract of stem of Mahonia nepalensis resulted in isolation of four pure compounds MN1, MN2, MN3 and MN4. Out of four isolated compounds, two were identified as MN1:b- sitosterol and MN2: Berberine by comparison of melting point, Co-TLC, IR and UV spectra of authentic sample. Potent pharmacological activity of Mahonia nepalensis and Berberis aristata were revealed from antimicrobial activity and brine shrimp bioassay. Methanol extracts of stem of Mahonia nepalensis and Berberis aristata showed significant zone of inhibition of 18 mm and 21 mm respectively against the Staphylococcus aureus. Methanol extract of Berberis aristata were comparatively little stronger against Staphylococcus aureus than methanol extract of Mahonia nepalensis. LC50 values (μg/ml) of methanol extracts of stem of Berberis aristata and Mahonia nepalensis were found to be 8.058x10-4 and 8.3 whereas methanol extracts of leaf of Mahonia nepalensis and Berberis aristata were 389.04 and 1303.166 respectively.mahonia nepalensisphytochemicalberberis aristatalc50 values |
| spellingShingle | Rojeena Thusa Sushika Mulmi Analysis of Phytoconstituents and Biological Activities of Different Parts of Mahonia nepalensis and Berberis aristata Nepal Journal of Biotechnology mahonia nepalensis phytochemical berberis aristata lc50 values |
| title | Analysis of Phytoconstituents and Biological Activities of Different Parts of Mahonia nepalensis and Berberis aristata |
| title_full | Analysis of Phytoconstituents and Biological Activities of Different Parts of Mahonia nepalensis and Berberis aristata |
| title_fullStr | Analysis of Phytoconstituents and Biological Activities of Different Parts of Mahonia nepalensis and Berberis aristata |
| title_full_unstemmed | Analysis of Phytoconstituents and Biological Activities of Different Parts of Mahonia nepalensis and Berberis aristata |
| title_short | Analysis of Phytoconstituents and Biological Activities of Different Parts of Mahonia nepalensis and Berberis aristata |
| title_sort | analysis of phytoconstituents and biological activities of different parts of mahonia nepalensis and berberis aristata |
| topic | mahonia nepalensis phytochemical berberis aristata lc50 values |
| work_keys_str_mv | AT rojeenathusa analysisofphytoconstituentsandbiologicalactivitiesofdifferentpartsofmahonianepalensisandberberisaristata AT sushikamulmi analysisofphytoconstituentsandbiologicalactivitiesofdifferentpartsofmahonianepalensisandberberisaristata |