Early Effects of Metabolic Syndrome on ATP-Sensitive Potassium Channels from Rat Pancreatic Beta Cells
Metabolic syndrome (MS) is a cluster of metabolic signs that increases the risk of developing type 2 two diabetes mellitus and cardiovascular diseases. MS leads to pancreatic beta cell exhaustion and decreased insulin secretion through unknown mechanisms in a time-dependent manner. ATP-sensitive pot...
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MDPI AG
2022-04-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/12/4/365 |
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| author | Iskra Cruz-Cruz Germán Bernate-Obando Carlos Larqué Rene Escalona Rodolfo Pinto-Almazán Myrian Velasco |
| author_facet | Iskra Cruz-Cruz Germán Bernate-Obando Carlos Larqué Rene Escalona Rodolfo Pinto-Almazán Myrian Velasco |
| author_sort | Iskra Cruz-Cruz |
| collection | DOAJ |
| description | Metabolic syndrome (MS) is a cluster of metabolic signs that increases the risk of developing type 2 two diabetes mellitus and cardiovascular diseases. MS leads to pancreatic beta cell exhaustion and decreased insulin secretion through unknown mechanisms in a time-dependent manner. ATP-sensitive potassium channels (K<sub>ATP</sub> channels), common targets of anti-diabetic drugs, participate in the glucose-stimulated insulin secretion, coupling the metabolic status and electrical activity of pancreatic beta cells. We investigated the early effects of MS on the conductance, ATP and glybenclamide sensitivity of the K<sub>ATP</sub> channels. We used <i>Wistar</i> rats fed with a high-sucrose diet (HSD) for 8 weeks as a MS model. In excised membrane patches, control and HSD channels showed similar unitary conductance and ATP sensitivity pancreatic beta cells in their K<sub>ATP</sub> channels<sub>.</sub> In contrast, MS produced variability in the sensitivity to glybenclamide of K<sub>ATP</sub> channels. We observed two subpopulations of pancreatic beta cells, one with similar (Gly1) and one with increased (Gly2) glybenclamide sensitivity compared to the control group. This study shows that the early effects of MS produced by consuming high-sugar beverages can affect the pharmacological properties of K<sub>ATP</sub> channels to one of the drugs used for diabetes treatment. |
| first_indexed | 2024-03-09T04:23:52Z |
| format | Article |
| id | doaj.art-574bd02788d240e3b321c81a482abe63 |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-09T04:23:52Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-574bd02788d240e3b321c81a482abe632023-12-03T13:43:11ZengMDPI AGMetabolites2218-19892022-04-0112436510.3390/metabo12040365Early Effects of Metabolic Syndrome on ATP-Sensitive Potassium Channels from Rat Pancreatic Beta CellsIskra Cruz-Cruz0Germán Bernate-Obando1Carlos Larqué2Rene Escalona3Rodolfo Pinto-Almazán4Myrian Velasco5Neuroscience Division, Department of Cognitive Neuroscience, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Ciudad Universitaria, AP 70-253 Coyoacán, México City 04510, MexicoNeuroscience Division, Department of Cognitive Neuroscience, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Ciudad Universitaria, AP 70-253 Coyoacán, México City 04510, MexicoEmbriology and Genetics Deparment, Facultad de Medicina, Universidad Nacional Autónoma de México, Ciudad Universitaria, AP 70-253 Coyoacán, México City 04510, MexicoEmbriology and Genetics Deparment, Facultad de Medicina, Universidad Nacional Autónoma de México, Ciudad Universitaria, AP 70-253 Coyoacán, México City 04510, MexicoNon-Communicable Disease Research Group, Facultad Mexicana de Medicina, Universidad La Salle-México, Las Fuentes 17, Tlalpan Centro I, Tlalpan, México City 14000, MexicoNeuroscience Division, Department of Cognitive Neuroscience, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Ciudad Universitaria, AP 70-253 Coyoacán, México City 04510, MexicoMetabolic syndrome (MS) is a cluster of metabolic signs that increases the risk of developing type 2 two diabetes mellitus and cardiovascular diseases. MS leads to pancreatic beta cell exhaustion and decreased insulin secretion through unknown mechanisms in a time-dependent manner. ATP-sensitive potassium channels (K<sub>ATP</sub> channels), common targets of anti-diabetic drugs, participate in the glucose-stimulated insulin secretion, coupling the metabolic status and electrical activity of pancreatic beta cells. We investigated the early effects of MS on the conductance, ATP and glybenclamide sensitivity of the K<sub>ATP</sub> channels. We used <i>Wistar</i> rats fed with a high-sucrose diet (HSD) for 8 weeks as a MS model. In excised membrane patches, control and HSD channels showed similar unitary conductance and ATP sensitivity pancreatic beta cells in their K<sub>ATP</sub> channels<sub>.</sub> In contrast, MS produced variability in the sensitivity to glybenclamide of K<sub>ATP</sub> channels. We observed two subpopulations of pancreatic beta cells, one with similar (Gly1) and one with increased (Gly2) glybenclamide sensitivity compared to the control group. This study shows that the early effects of MS produced by consuming high-sugar beverages can affect the pharmacological properties of K<sub>ATP</sub> channels to one of the drugs used for diabetes treatment.https://www.mdpi.com/2218-1989/12/4/365metabolic syndromeK<sub>ATP</sub> channelsglybenclamidepancreatic beta cellinsulin |
| spellingShingle | Iskra Cruz-Cruz Germán Bernate-Obando Carlos Larqué Rene Escalona Rodolfo Pinto-Almazán Myrian Velasco Early Effects of Metabolic Syndrome on ATP-Sensitive Potassium Channels from Rat Pancreatic Beta Cells Metabolites metabolic syndrome K<sub>ATP</sub> channels glybenclamide pancreatic beta cell insulin |
| title | Early Effects of Metabolic Syndrome on ATP-Sensitive Potassium Channels from Rat Pancreatic Beta Cells |
| title_full | Early Effects of Metabolic Syndrome on ATP-Sensitive Potassium Channels from Rat Pancreatic Beta Cells |
| title_fullStr | Early Effects of Metabolic Syndrome on ATP-Sensitive Potassium Channels from Rat Pancreatic Beta Cells |
| title_full_unstemmed | Early Effects of Metabolic Syndrome on ATP-Sensitive Potassium Channels from Rat Pancreatic Beta Cells |
| title_short | Early Effects of Metabolic Syndrome on ATP-Sensitive Potassium Channels from Rat Pancreatic Beta Cells |
| title_sort | early effects of metabolic syndrome on atp sensitive potassium channels from rat pancreatic beta cells |
| topic | metabolic syndrome K<sub>ATP</sub> channels glybenclamide pancreatic beta cell insulin |
| url | https://www.mdpi.com/2218-1989/12/4/365 |
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