Effects of dietary spermine supplementation on cell cycle, apoptosis, and amino acid transporters of the thymus and spleen in piglets
Objective This study investigated whether spermine supplementation could regulate cell cycle, apoptosis, and amino acid transporter-related genes expression in the thymus and spleen of early weaned piglets. Methods Eighty female piglets were randomly distributed to receive adequate nutrients supplem...
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| Format: | Article |
| Language: | English |
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Asian-Australasian Association of Animal Production Societies
2018-08-01
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| Series: | Asian-Australasian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | http://www.ajas.info/upload/pdf/ajas-31-8-1325.pdf |
| _version_ | 1811240274287919104 |
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| author | Wei Cao Xianjian Wu Gang Jia Hua Zhao Xiaoling Chen Caimei Wu Jingyi Cai Jing Wang Guangmang Liu |
| author_facet | Wei Cao Xianjian Wu Gang Jia Hua Zhao Xiaoling Chen Caimei Wu Jingyi Cai Jing Wang Guangmang Liu |
| author_sort | Wei Cao |
| collection | DOAJ |
| description | Objective This study investigated whether spermine supplementation could regulate cell cycle, apoptosis, and amino acid transporter-related genes expression in the thymus and spleen of early weaned piglets. Methods Eighty female piglets were randomly distributed to receive adequate nutrients supplemented with spermine (0.4 mmol/kg body weight/24 h) or to be provided with restricted nourishment supplemented with normal saline for 7 h or 3, 6, or 9 d in pairs. Results Regardless of administration time, spermine supplementation significantly up-regulated cyclin A2 gene expression but down-regulated p21 and cyclin D3 mRNA levels in the thymus and spleen and reduced cyclin E2 gene expression in the thymus of piglets (p< 0.05). Irrespective of the treatment period, the reduced Bax and caspase-3 gene expressions and improved Bcl-2 mRNA level were observed in the thymus and spleen of spermine-administrated piglets (p<0.05). Regardless of supplementation time, spermine intake significantly enhanced the expressions of amino acid transporter-related genes (SLC1A1, SLC1A5, SLC7A1, SLC7A7, and SLC15A1) in both thymus and spleen, as well as SLC7A9 in the spleen of piglets (p<0.05). In addition, extended spermine administration also markedly promoted cell proliferation, depressed apoptosis and modulated amino acid transport (p< 0.05), and such effects were the greatest during prolonged spermine supplementation (6 d) compared to the other time periods (p<0.05). Conclusion Spermine supplementation may regulate cell cycle during the G1/S phase, suppress apoptosis and modulate amino acid transport. A period of 6 d of spermine supplementation is required to produce the optimal effects on nutritional implications. |
| first_indexed | 2024-04-12T13:16:26Z |
| format | Article |
| id | doaj.art-574d1591ae08460f8d3b8cc847a78059 |
| institution | Directory Open Access Journal |
| issn | 1011-2367 1976-5517 |
| language | English |
| last_indexed | 2024-04-12T13:16:26Z |
| publishDate | 2018-08-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Asian-Australasian Journal of Animal Sciences |
| spelling | doaj.art-574d1591ae08460f8d3b8cc847a780592022-12-22T03:31:40ZengAsian-Australasian Association of Animal Production SocietiesAsian-Australasian Journal of Animal Sciences1011-23671976-55172018-08-013181325133510.5713/ajas.17.079923920Effects of dietary spermine supplementation on cell cycle, apoptosis, and amino acid transporters of the thymus and spleen in pigletsWei Cao0Xianjian Wu1Gang Jia2Hua Zhao3Xiaoling Chen4Caimei Wu5Jingyi Cai6Jing Wang7Guangmang Liu8 Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, China Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, China Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, China Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, China Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, China Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, China Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, China Maize Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, ChinaObjective This study investigated whether spermine supplementation could regulate cell cycle, apoptosis, and amino acid transporter-related genes expression in the thymus and spleen of early weaned piglets. Methods Eighty female piglets were randomly distributed to receive adequate nutrients supplemented with spermine (0.4 mmol/kg body weight/24 h) or to be provided with restricted nourishment supplemented with normal saline for 7 h or 3, 6, or 9 d in pairs. Results Regardless of administration time, spermine supplementation significantly up-regulated cyclin A2 gene expression but down-regulated p21 and cyclin D3 mRNA levels in the thymus and spleen and reduced cyclin E2 gene expression in the thymus of piglets (p< 0.05). Irrespective of the treatment period, the reduced Bax and caspase-3 gene expressions and improved Bcl-2 mRNA level were observed in the thymus and spleen of spermine-administrated piglets (p<0.05). Regardless of supplementation time, spermine intake significantly enhanced the expressions of amino acid transporter-related genes (SLC1A1, SLC1A5, SLC7A1, SLC7A7, and SLC15A1) in both thymus and spleen, as well as SLC7A9 in the spleen of piglets (p<0.05). In addition, extended spermine administration also markedly promoted cell proliferation, depressed apoptosis and modulated amino acid transport (p< 0.05), and such effects were the greatest during prolonged spermine supplementation (6 d) compared to the other time periods (p<0.05). Conclusion Spermine supplementation may regulate cell cycle during the G1/S phase, suppress apoptosis and modulate amino acid transport. A period of 6 d of spermine supplementation is required to produce the optimal effects on nutritional implications.http://www.ajas.info/upload/pdf/ajas-31-8-1325.pdfSpermineCell CycleApoptosisAmino Acid TransportersPiglet |
| spellingShingle | Wei Cao Xianjian Wu Gang Jia Hua Zhao Xiaoling Chen Caimei Wu Jingyi Cai Jing Wang Guangmang Liu Effects of dietary spermine supplementation on cell cycle, apoptosis, and amino acid transporters of the thymus and spleen in piglets Asian-Australasian Journal of Animal Sciences Spermine Cell Cycle Apoptosis Amino Acid Transporters Piglet |
| title | Effects of dietary spermine supplementation on cell cycle, apoptosis, and amino acid transporters of the thymus and spleen in piglets |
| title_full | Effects of dietary spermine supplementation on cell cycle, apoptosis, and amino acid transporters of the thymus and spleen in piglets |
| title_fullStr | Effects of dietary spermine supplementation on cell cycle, apoptosis, and amino acid transporters of the thymus and spleen in piglets |
| title_full_unstemmed | Effects of dietary spermine supplementation on cell cycle, apoptosis, and amino acid transporters of the thymus and spleen in piglets |
| title_short | Effects of dietary spermine supplementation on cell cycle, apoptosis, and amino acid transporters of the thymus and spleen in piglets |
| title_sort | effects of dietary spermine supplementation on cell cycle apoptosis and amino acid transporters of the thymus and spleen in piglets |
| topic | Spermine Cell Cycle Apoptosis Amino Acid Transporters Piglet |
| url | http://www.ajas.info/upload/pdf/ajas-31-8-1325.pdf |
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