Expression of apoptosis-Related genes bcl-2 and bax in rat brain hippocampus, followed by intraperitoneal injection of nanosilver
Background: Silver nanoparticles are small scale substance (<100 nm) used in food technology and medical industry. The data suggest that nanosilver may produce neurotoxicity by generating free radical-induced oxidative stress and by altering gene expression producing apoptosis and neurotoxicity....
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| Format: | Article |
| Language: | English |
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Bushehr University of Medical Sciences
2016-05-01
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| Series: | Iranian South Medical Journal |
| Subjects: | |
| Online Access: | http://ismj.bpums.ac.ir/browse.php?a_code=A-10-3-685&slc_lang=en&sid=1 |
| _version_ | 1828918052281909248 |
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| author | Maryam Ghoshcian Parvin Khodarahmi Farzaneh Tafvizi |
| author_facet | Maryam Ghoshcian Parvin Khodarahmi Farzaneh Tafvizi |
| author_sort | Maryam Ghoshcian |
| collection | DOAJ |
| description | Background: Silver nanoparticles are small scale substance (<100 nm) used in food technology and medical industry. The data suggest that nanosilver may produce neurotoxicity by generating free radical-induced oxidative stress and by altering gene expression producing apoptosis and neurotoxicity. In this study, the apoptotic effects of Nano silver on apoptosis- related genes expression bcl-2 and bax on rat hippocampus, which is involved in memory and learning, was investigated.
Materials & Methods: 28 male Wistar rats were divided into four groups of control and three groups of the treatment. The control group received saline and the treatment groups received intraperitoneal injections of silver nanoparticles at doses of 100, 200 and 400ppm. Ten days after the last injection, the hippocampal region was dissected and removed and then the expression of bcl-2 and bax genes was evaluated using semi-qualitative RT-PCR and Densitometry assay.
Results: The expression of anti- apoptotic b-cl2 gene was reduced in the treatment groups compared to the control group. In comparison, the expression of pro- apoptotic bax gene was increased in the treatment groups compared to the control group. This apoptotic affects was increased at higher doses.
Conclusion: The data suggest that silver nanoparticles may produce apoptosis by altering apoptosis- related genes expression, in rat brain hippocampus cells. |
| first_indexed | 2024-12-13T21:02:36Z |
| format | Article |
| id | doaj.art-574d850c84c84e6eba433a76ae32a239 |
| institution | Directory Open Access Journal |
| issn | 1735-4374 1735-6954 |
| language | English |
| last_indexed | 2024-12-13T21:02:36Z |
| publishDate | 2016-05-01 |
| publisher | Bushehr University of Medical Sciences |
| record_format | Article |
| series | Iranian South Medical Journal |
| spelling | doaj.art-574d850c84c84e6eba433a76ae32a2392022-12-21T23:31:33ZengBushehr University of Medical SciencesIranian South Medical Journal1735-43741735-69542016-05-01192185193Expression of apoptosis-Related genes bcl-2 and bax in rat brain hippocampus, followed by intraperitoneal injection of nanosilverMaryam Ghoshcian0Parvin Khodarahmi1Farzaneh Tafvizi2 Department of biology, Branch of Parand, Islamic Azad University, Parand, Iran Department of biology, Branch of Parand, Islamic Azad University, Parand, Iran Department of biology, Branch of Parand, Islamic Azad University, Parand, Iran Background: Silver nanoparticles are small scale substance (<100 nm) used in food technology and medical industry. The data suggest that nanosilver may produce neurotoxicity by generating free radical-induced oxidative stress and by altering gene expression producing apoptosis and neurotoxicity. In this study, the apoptotic effects of Nano silver on apoptosis- related genes expression bcl-2 and bax on rat hippocampus, which is involved in memory and learning, was investigated. Materials & Methods: 28 male Wistar rats were divided into four groups of control and three groups of the treatment. The control group received saline and the treatment groups received intraperitoneal injections of silver nanoparticles at doses of 100, 200 and 400ppm. Ten days after the last injection, the hippocampal region was dissected and removed and then the expression of bcl-2 and bax genes was evaluated using semi-qualitative RT-PCR and Densitometry assay. Results: The expression of anti- apoptotic b-cl2 gene was reduced in the treatment groups compared to the control group. In comparison, the expression of pro- apoptotic bax gene was increased in the treatment groups compared to the control group. This apoptotic affects was increased at higher doses. Conclusion: The data suggest that silver nanoparticles may produce apoptosis by altering apoptosis- related genes expression, in rat brain hippocampus cells.http://ismj.bpums.ac.ir/browse.php?a_code=A-10-3-685&slc_lang=en&sid=1silver nanoparticles bcl-2 gene bax gene semi-qualitative RT-PCR Densitometry |
| spellingShingle | Maryam Ghoshcian Parvin Khodarahmi Farzaneh Tafvizi Expression of apoptosis-Related genes bcl-2 and bax in rat brain hippocampus, followed by intraperitoneal injection of nanosilver Iranian South Medical Journal silver nanoparticles bcl-2 gene bax gene semi-qualitative RT-PCR Densitometry |
| title | Expression of apoptosis-Related genes bcl-2 and bax in rat brain hippocampus, followed by intraperitoneal injection of nanosilver |
| title_full | Expression of apoptosis-Related genes bcl-2 and bax in rat brain hippocampus, followed by intraperitoneal injection of nanosilver |
| title_fullStr | Expression of apoptosis-Related genes bcl-2 and bax in rat brain hippocampus, followed by intraperitoneal injection of nanosilver |
| title_full_unstemmed | Expression of apoptosis-Related genes bcl-2 and bax in rat brain hippocampus, followed by intraperitoneal injection of nanosilver |
| title_short | Expression of apoptosis-Related genes bcl-2 and bax in rat brain hippocampus, followed by intraperitoneal injection of nanosilver |
| title_sort | expression of apoptosis related genes bcl 2 and bax in rat brain hippocampus followed by intraperitoneal injection of nanosilver |
| topic | silver nanoparticles bcl-2 gene bax gene semi-qualitative RT-PCR Densitometry |
| url | http://ismj.bpums.ac.ir/browse.php?a_code=A-10-3-685&slc_lang=en&sid=1 |
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