A Method for Whole Brain Ex Vivo Magnetic Resonance Imaging with Minimal Susceptibility Artifacts
Magnetic resonance imaging (MRI) is a non-destructive technique that is capable of localizing pathologies and assessing other anatomical features (e.g., tissue volume, microstructure, white matter connectivity) in postmortem, ex vivo human brains. However, when brains are removed from the skull and...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-11-01
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| Series: | Frontiers in Neurology |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fneur.2016.00208/full |
| _version_ | 1818561509256593408 |
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| author | Anwar S. Shatil Anwar S. Shatil Kant M. Matsuda Kant M. Matsuda Chase R. Figley Chase R. Figley Chase R. Figley Chase R. Figley Chase R. Figley |
| author_facet | Anwar S. Shatil Anwar S. Shatil Kant M. Matsuda Kant M. Matsuda Chase R. Figley Chase R. Figley Chase R. Figley Chase R. Figley Chase R. Figley |
| author_sort | Anwar S. Shatil |
| collection | DOAJ |
| description | Magnetic resonance imaging (MRI) is a non-destructive technique that is capable of localizing pathologies and assessing other anatomical features (e.g., tissue volume, microstructure, white matter connectivity) in postmortem, ex vivo human brains. However, when brains are removed from the skull and cerebrospinal fluid (i.e., their normal in vivo magnetic environment), air bubbles and air-tissue interfaces typically cause magnetic susceptibility artifacts that severely degrade the quality of ex vivo MRI data. In this report, we describe a relatively simple and cost-effective experimental set-up for acquiring artifact-free ex vivo brain images using a clinical MRI system with standard hardware. In particular, we outline the necessary steps, from collecting an ex vivo human brain to the MRI scanner setup, and have also described changing the formalin (as might be necessary in longitudinal postmortem studies). Finally, we share some representative ex vivo MRI images that have been acquired using the proposed setup in order to demonstrate the efficacy of this approach. We hope that this protocol will provide both clinicians and researchers with a straight-forward and cost-effective solution for acquiring ex vivo MRI data from whole postmortem human brains. |
| first_indexed | 2024-12-14T00:51:47Z |
| format | Article |
| id | doaj.art-574e1303a3274c5a92fafa49fa84a96d |
| institution | Directory Open Access Journal |
| issn | 1664-2295 |
| language | English |
| last_indexed | 2024-12-14T00:51:47Z |
| publishDate | 2016-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neurology |
| spelling | doaj.art-574e1303a3274c5a92fafa49fa84a96d2022-12-21T23:23:47ZengFrontiers Media S.A.Frontiers in Neurology1664-22952016-11-01710.3389/fneur.2016.00208222868A Method for Whole Brain Ex Vivo Magnetic Resonance Imaging with Minimal Susceptibility ArtifactsAnwar S. Shatil0Anwar S. Shatil1Kant M. Matsuda2Kant M. Matsuda3Chase R. Figley4Chase R. Figley5Chase R. Figley6Chase R. Figley7Chase R. Figley8University of ManitobaKleysen Institute for Advanced MedicineUniversity of ManitobaUniversity of ManitobaUniversity of ManitobaUniversity of ManitobaKleysen Institute for Advanced MedicineHealth Sciences CentreJohns Hopkins UniversityMagnetic resonance imaging (MRI) is a non-destructive technique that is capable of localizing pathologies and assessing other anatomical features (e.g., tissue volume, microstructure, white matter connectivity) in postmortem, ex vivo human brains. However, when brains are removed from the skull and cerebrospinal fluid (i.e., their normal in vivo magnetic environment), air bubbles and air-tissue interfaces typically cause magnetic susceptibility artifacts that severely degrade the quality of ex vivo MRI data. In this report, we describe a relatively simple and cost-effective experimental set-up for acquiring artifact-free ex vivo brain images using a clinical MRI system with standard hardware. In particular, we outline the necessary steps, from collecting an ex vivo human brain to the MRI scanner setup, and have also described changing the formalin (as might be necessary in longitudinal postmortem studies). Finally, we share some representative ex vivo MRI images that have been acquired using the proposed setup in order to demonstrate the efficacy of this approach. We hope that this protocol will provide both clinicians and researchers with a straight-forward and cost-effective solution for acquiring ex vivo MRI data from whole postmortem human brains.http://journal.frontiersin.org/Journal/10.3389/fneur.2016.00208/fullNeuroimaginghuman brainMRIex vivofixationpostmortem |
| spellingShingle | Anwar S. Shatil Anwar S. Shatil Kant M. Matsuda Kant M. Matsuda Chase R. Figley Chase R. Figley Chase R. Figley Chase R. Figley Chase R. Figley A Method for Whole Brain Ex Vivo Magnetic Resonance Imaging with Minimal Susceptibility Artifacts Frontiers in Neurology Neuroimaging human brain MRI ex vivo fixation postmortem |
| title | A Method for Whole Brain Ex Vivo Magnetic Resonance Imaging with Minimal Susceptibility Artifacts |
| title_full | A Method for Whole Brain Ex Vivo Magnetic Resonance Imaging with Minimal Susceptibility Artifacts |
| title_fullStr | A Method for Whole Brain Ex Vivo Magnetic Resonance Imaging with Minimal Susceptibility Artifacts |
| title_full_unstemmed | A Method for Whole Brain Ex Vivo Magnetic Resonance Imaging with Minimal Susceptibility Artifacts |
| title_short | A Method for Whole Brain Ex Vivo Magnetic Resonance Imaging with Minimal Susceptibility Artifacts |
| title_sort | method for whole brain ex vivo magnetic resonance imaging with minimal susceptibility artifacts |
| topic | Neuroimaging human brain MRI ex vivo fixation postmortem |
| url | http://journal.frontiersin.org/Journal/10.3389/fneur.2016.00208/full |
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