The transcriptome of Exophiala dermatitidis during ex-vivo kin model infection
The black yeast Exophiala dermatitidis is a widespread polyextremophile and humanpathogen, that is found in extreme natural habitats and man-made environments suchas dishwashers. It can cause various diseases ranging from phaehyphomycosis andsystemic infections, with fatality rates reaching 40%. Whi...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-10-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fcimb.2016.00136/full |
| _version_ | 1818360552369422336 |
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| author | Caroline Poyntner Barbara Blasi Elsa Arcalis Ursula Mirastschijski Ursula Mirastschijski Katja Sterflinger Hakim Tafer |
| author_facet | Caroline Poyntner Barbara Blasi Elsa Arcalis Ursula Mirastschijski Ursula Mirastschijski Katja Sterflinger Hakim Tafer |
| author_sort | Caroline Poyntner |
| collection | DOAJ |
| description | The black yeast Exophiala dermatitidis is a widespread polyextremophile and humanpathogen, that is found in extreme natural habitats and man-made environments suchas dishwashers. It can cause various diseases ranging from phaehyphomycosis andsystemic infections, with fatality rates reaching 40%. While the number of cases inimmunocompromised patients are increasing, knowledge of the infections, virulencefactors and host response is still scarce. In this study, for the first time, an artificialinfection of an ex-vivo skin model with Exophiala dermatitidis was monitoredmicroscopically and transcriptomically. Results show that Exophiala dermatitidis is ableto actively grow and penetrate the skin. The analysis of the genomic andRNA-sequencing data delivers a rich and complex transcriptome where circular RNAs,fusion transcripts, long non-coding RNAs and antisense transcripts are found. Changesin transcription strongly affect pathways related to nutrients acquisition, energymetabolism, cell wall, morphological switch, and known virulence factors. TheL-Tyrosine melanin pathway is specifically upregulated during infection. Moreover theproduction of secondary metabolites, especially alkaloids, is icreased. Our study is thefirst that gives an insight into the complexity of the transcriptome of Exophialadermatitidis during artificial skin infections and reveals new virulence factors. |
| first_indexed | 2024-12-13T21:02:36Z |
| format | Article |
| id | doaj.art-57517a1d560c4104a26f5788b59d0a7c |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-13T21:02:36Z |
| publishDate | 2016-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-57517a1d560c4104a26f5788b59d0a7c2022-12-21T23:31:33ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882016-10-01610.3389/fcimb.2016.00136219795The transcriptome of Exophiala dermatitidis during ex-vivo kin model infectionCaroline Poyntner0Barbara Blasi1Elsa Arcalis2Ursula Mirastschijski3Ursula Mirastschijski4Katja Sterflinger5Hakim Tafer6University of Natural Resources and Life SciencesUniversity of Natural Resources and Life SciencesUniversity of Natural Resources and Life SciencesKlinikum Bremen-MitteFaculty of Biology and ChemistryUniversity of Natural Resources and Life SciencesUniversity of Natural Resources and Life SciencesThe black yeast Exophiala dermatitidis is a widespread polyextremophile and humanpathogen, that is found in extreme natural habitats and man-made environments suchas dishwashers. It can cause various diseases ranging from phaehyphomycosis andsystemic infections, with fatality rates reaching 40%. While the number of cases inimmunocompromised patients are increasing, knowledge of the infections, virulencefactors and host response is still scarce. In this study, for the first time, an artificialinfection of an ex-vivo skin model with Exophiala dermatitidis was monitoredmicroscopically and transcriptomically. Results show that Exophiala dermatitidis is ableto actively grow and penetrate the skin. The analysis of the genomic andRNA-sequencing data delivers a rich and complex transcriptome where circular RNAs,fusion transcripts, long non-coding RNAs and antisense transcripts are found. Changesin transcription strongly affect pathways related to nutrients acquisition, energymetabolism, cell wall, morphological switch, and known virulence factors. TheL-Tyrosine melanin pathway is specifically upregulated during infection. Moreover theproduction of secondary metabolites, especially alkaloids, is icreased. Our study is thefirst that gives an insight into the complexity of the transcriptome of Exophialadermatitidis during artificial skin infections and reveals new virulence factors.http://journal.frontiersin.org/Journal/10.3389/fcimb.2016.00136/fullVirulenceFungal pathogensSkin InfectionSkin ModelBlack yeastRNA sequencing (RNA-Seq) |
| spellingShingle | Caroline Poyntner Barbara Blasi Elsa Arcalis Ursula Mirastschijski Ursula Mirastschijski Katja Sterflinger Hakim Tafer The transcriptome of Exophiala dermatitidis during ex-vivo kin model infection Frontiers in Cellular and Infection Microbiology Virulence Fungal pathogens Skin Infection Skin Model Black yeast RNA sequencing (RNA-Seq) |
| title | The transcriptome of Exophiala dermatitidis during ex-vivo kin model infection |
| title_full | The transcriptome of Exophiala dermatitidis during ex-vivo kin model infection |
| title_fullStr | The transcriptome of Exophiala dermatitidis during ex-vivo kin model infection |
| title_full_unstemmed | The transcriptome of Exophiala dermatitidis during ex-vivo kin model infection |
| title_short | The transcriptome of Exophiala dermatitidis during ex-vivo kin model infection |
| title_sort | transcriptome of exophiala dermatitidis during ex vivo kin model infection |
| topic | Virulence Fungal pathogens Skin Infection Skin Model Black yeast RNA sequencing (RNA-Seq) |
| url | http://journal.frontiersin.org/Journal/10.3389/fcimb.2016.00136/full |
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