Superhydrophilic Polyurethane/Polydopamine Nanofibrous Materials Enhancing Cell Adhesion for Application in Tissue Engineering
The use of nanofibrous materials in the field of tissue engineering requires a fast, efficient, scalable production method and excellent wettability of the obtained materials, leading to enhanced cell adhesion. We proposed the production method of superhydrophilic nanofibrous materials in a two-step...
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| Format: | Article |
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MDPI AG
2020-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/18/6798 |
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| author | Kamil Kopeć Michał Wojasiński Tomasz Ciach |
| author_facet | Kamil Kopeć Michał Wojasiński Tomasz Ciach |
| author_sort | Kamil Kopeć |
| collection | DOAJ |
| description | The use of nanofibrous materials in the field of tissue engineering requires a fast, efficient, scalable production method and excellent wettability of the obtained materials, leading to enhanced cell adhesion. We proposed the production method of superhydrophilic nanofibrous materials in a two-step process. The process is designed to increase the wettability of resulting scaffolds and to enhance the rate of fibroblast cell adhesion. Polyurethane (PU) nanofibrous material was produced in the solution blow spinning process. Then the PU fibers surface was modified by dopamine polymerization in water solution. Two variants of the modification were examined: dopamine polymerization under atmospheric oxygen (V-I) and using sodium periodate as an oxidative agent (V-II). Hydrophobic PU materials after the treatment became highly hydrophilic, regardless of the modification variant. This effect originates from polydopamine (PDA) coating properties and nanoscale surface structures. The modification improved the mechanical properties of the materials. Materials obtained in the V-II process exhibit superior properties over those from the V-I, and require shorter modification time (less than 30 min). Modifications significantly improved fibroblasts adhesion. The cells spread after 2 h on both PDA-modified PU nanofibrous materials, which was not observed for unmodified PU. Proposed technology could be beneficial in applications like scaffolds for tissue engineering. |
| first_indexed | 2024-03-10T16:17:31Z |
| format | Article |
| id | doaj.art-5752f696357a4788819768c19d9b252c |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T16:17:31Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-5752f696357a4788819768c19d9b252c2023-11-20T13:56:51ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-09-012118679810.3390/ijms21186798Superhydrophilic Polyurethane/Polydopamine Nanofibrous Materials Enhancing Cell Adhesion for Application in Tissue EngineeringKamil Kopeć0Michał Wojasiński1Tomasz Ciach2Faculty of Chemical and Process Engineering, Biomedical Engineering Laboratory, Warsaw University of Technology, Waryńskiego 1, 00-645 Warsaw, PolandFaculty of Chemical and Process Engineering, Biomedical Engineering Laboratory, Warsaw University of Technology, Waryńskiego 1, 00-645 Warsaw, PolandFaculty of Chemical and Process Engineering, Biomedical Engineering Laboratory, Warsaw University of Technology, Waryńskiego 1, 00-645 Warsaw, PolandThe use of nanofibrous materials in the field of tissue engineering requires a fast, efficient, scalable production method and excellent wettability of the obtained materials, leading to enhanced cell adhesion. We proposed the production method of superhydrophilic nanofibrous materials in a two-step process. The process is designed to increase the wettability of resulting scaffolds and to enhance the rate of fibroblast cell adhesion. Polyurethane (PU) nanofibrous material was produced in the solution blow spinning process. Then the PU fibers surface was modified by dopamine polymerization in water solution. Two variants of the modification were examined: dopamine polymerization under atmospheric oxygen (V-I) and using sodium periodate as an oxidative agent (V-II). Hydrophobic PU materials after the treatment became highly hydrophilic, regardless of the modification variant. This effect originates from polydopamine (PDA) coating properties and nanoscale surface structures. The modification improved the mechanical properties of the materials. Materials obtained in the V-II process exhibit superior properties over those from the V-I, and require shorter modification time (less than 30 min). Modifications significantly improved fibroblasts adhesion. The cells spread after 2 h on both PDA-modified PU nanofibrous materials, which was not observed for unmodified PU. Proposed technology could be beneficial in applications like scaffolds for tissue engineering.https://www.mdpi.com/1422-0067/21/18/6798polydopaminenanofiberspolyurethanehydrophilizationtensile propertiesfibroblasts |
| spellingShingle | Kamil Kopeć Michał Wojasiński Tomasz Ciach Superhydrophilic Polyurethane/Polydopamine Nanofibrous Materials Enhancing Cell Adhesion for Application in Tissue Engineering International Journal of Molecular Sciences polydopamine nanofibers polyurethane hydrophilization tensile properties fibroblasts |
| title | Superhydrophilic Polyurethane/Polydopamine Nanofibrous Materials Enhancing Cell Adhesion for Application in Tissue Engineering |
| title_full | Superhydrophilic Polyurethane/Polydopamine Nanofibrous Materials Enhancing Cell Adhesion for Application in Tissue Engineering |
| title_fullStr | Superhydrophilic Polyurethane/Polydopamine Nanofibrous Materials Enhancing Cell Adhesion for Application in Tissue Engineering |
| title_full_unstemmed | Superhydrophilic Polyurethane/Polydopamine Nanofibrous Materials Enhancing Cell Adhesion for Application in Tissue Engineering |
| title_short | Superhydrophilic Polyurethane/Polydopamine Nanofibrous Materials Enhancing Cell Adhesion for Application in Tissue Engineering |
| title_sort | superhydrophilic polyurethane polydopamine nanofibrous materials enhancing cell adhesion for application in tissue engineering |
| topic | polydopamine nanofibers polyurethane hydrophilization tensile properties fibroblasts |
| url | https://www.mdpi.com/1422-0067/21/18/6798 |
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