Identification of co-expressed central genes and transcription factors in atherosclerosis-related intracranial aneurysm
BackgroundNumerous clinical studies have shown that atherosclerosis is one of the risk factors for intracranial aneurysms. Calcifications in the intracranial aneurysm walls are frequently correlated with atherosclerosis. However, the pathogenesis of atherosclerosis-related intracranial aneurysms rem...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Neurology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fneur.2023.1055456/full |
| _version_ | 1811161906777423872 |
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| author | Quan Zhang Hengfang Liu Min Zhang Fang Liu Tiantian Liu |
| author_facet | Quan Zhang Hengfang Liu Min Zhang Fang Liu Tiantian Liu |
| author_sort | Quan Zhang |
| collection | DOAJ |
| description | BackgroundNumerous clinical studies have shown that atherosclerosis is one of the risk factors for intracranial aneurysms. Calcifications in the intracranial aneurysm walls are frequently correlated with atherosclerosis. However, the pathogenesis of atherosclerosis-related intracranial aneurysms remains unclear. This study aims to investigate this mechanism.MethodsThe Gene Expression Omnibus (GEO) database was used to download the gene expression profiles for atherosclerosis (GSE100927) and intracranial aneurysms (GSE75436). Following the identification of the common differentially expressed genes (DEGs) of atherosclerosis and intracranial aneurysm, the network creation of protein interactions, functional annotation, the identification of hub genes, and co-expression analysis were conducted. Thereafter, we predicted the transcription factors (TF) of hub genes and verified their expressions.ResultsA total of 270 common (62 downregulated and 208 upregulated) DEGs were identified for subsequent analysis. Functional analyses highlighted the significant role of phagocytosis, cytotoxicity, and T-cell receptor signaling pathways in this disease progression. Eight hub genes were identified and verified, namely, CCR5, FCGR3A, IL10RA, ITGAX, LCP2, PTPRC, TLR2, and TYROBP. Two TFs were also predicted and verified, which were IKZF1 and SPI1.ConclusionIntracranial aneurysms are correlated with atherosclerosis. We identified several hub genes for atherosclerosis-related intracranial aneurysms and explored the underlying pathogenesis. These discoveries may provide new insights for future experiments and clinical practice. |
| first_indexed | 2024-04-10T06:21:34Z |
| format | Article |
| id | doaj.art-57551489a3bd460c896a22a6efeb357f |
| institution | Directory Open Access Journal |
| issn | 1664-2295 |
| language | English |
| last_indexed | 2024-04-10T06:21:34Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neurology |
| spelling | doaj.art-57551489a3bd460c896a22a6efeb357f2023-03-02T04:56:11ZengFrontiers Media S.A.Frontiers in Neurology1664-22952023-03-011410.3389/fneur.2023.10554561055456Identification of co-expressed central genes and transcription factors in atherosclerosis-related intracranial aneurysmQuan Zhang0Hengfang Liu1Min Zhang2Fang Liu3Tiantian Liu4Department of Neurology, Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, ChinaDepartment of Neurology, Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, ChinaDepartment of Neurology, Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, ChinaDepartment of Neurology, Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, ChinaDepartment of Neurology, First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, ChinaBackgroundNumerous clinical studies have shown that atherosclerosis is one of the risk factors for intracranial aneurysms. Calcifications in the intracranial aneurysm walls are frequently correlated with atherosclerosis. However, the pathogenesis of atherosclerosis-related intracranial aneurysms remains unclear. This study aims to investigate this mechanism.MethodsThe Gene Expression Omnibus (GEO) database was used to download the gene expression profiles for atherosclerosis (GSE100927) and intracranial aneurysms (GSE75436). Following the identification of the common differentially expressed genes (DEGs) of atherosclerosis and intracranial aneurysm, the network creation of protein interactions, functional annotation, the identification of hub genes, and co-expression analysis were conducted. Thereafter, we predicted the transcription factors (TF) of hub genes and verified their expressions.ResultsA total of 270 common (62 downregulated and 208 upregulated) DEGs were identified for subsequent analysis. Functional analyses highlighted the significant role of phagocytosis, cytotoxicity, and T-cell receptor signaling pathways in this disease progression. Eight hub genes were identified and verified, namely, CCR5, FCGR3A, IL10RA, ITGAX, LCP2, PTPRC, TLR2, and TYROBP. Two TFs were also predicted and verified, which were IKZF1 and SPI1.ConclusionIntracranial aneurysms are correlated with atherosclerosis. We identified several hub genes for atherosclerosis-related intracranial aneurysms and explored the underlying pathogenesis. These discoveries may provide new insights for future experiments and clinical practice.https://www.frontiersin.org/articles/10.3389/fneur.2023.1055456/fullintracranial aneurysmsatherosclerosisbioinformaticsdifferentially expressed geneshub genestranscription factor |
| spellingShingle | Quan Zhang Hengfang Liu Min Zhang Fang Liu Tiantian Liu Identification of co-expressed central genes and transcription factors in atherosclerosis-related intracranial aneurysm Frontiers in Neurology intracranial aneurysms atherosclerosis bioinformatics differentially expressed genes hub genes transcription factor |
| title | Identification of co-expressed central genes and transcription factors in atherosclerosis-related intracranial aneurysm |
| title_full | Identification of co-expressed central genes and transcription factors in atherosclerosis-related intracranial aneurysm |
| title_fullStr | Identification of co-expressed central genes and transcription factors in atherosclerosis-related intracranial aneurysm |
| title_full_unstemmed | Identification of co-expressed central genes and transcription factors in atherosclerosis-related intracranial aneurysm |
| title_short | Identification of co-expressed central genes and transcription factors in atherosclerosis-related intracranial aneurysm |
| title_sort | identification of co expressed central genes and transcription factors in atherosclerosis related intracranial aneurysm |
| topic | intracranial aneurysms atherosclerosis bioinformatics differentially expressed genes hub genes transcription factor |
| url | https://www.frontiersin.org/articles/10.3389/fneur.2023.1055456/full |
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