Detection of Lipase Activity in Cells by a Fluorescent Probe Based on Formation of Self-Assembled Micelles
Summary: Reliable and sensitive detection of lipase activity is essential for the early diagnosis and monitoring of acute pancreatitis or progression of digestive diseases. However, the available fluorescent probes for detection of lipase activity are only implemented in a hexane-water two-phase sys...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-07-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004220304818 |
| _version_ | 1819289754372407296 |
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| author | Zhen Qiao Hongyi Zhang Yanru Zhang KeWei Wang |
| author_facet | Zhen Qiao Hongyi Zhang Yanru Zhang KeWei Wang |
| author_sort | Zhen Qiao |
| collection | DOAJ |
| description | Summary: Reliable and sensitive detection of lipase activity is essential for the early diagnosis and monitoring of acute pancreatitis or progression of digestive diseases. However, the available fluorescent probes for detection of lipase activity are only implemented in a hexane-water two-phase system due to the nature of heterogeneous catalysis of lipase, thus limiting their applications in direct imaging of lipase activity in living cells and tissues. Here we designed and synthesized a “turn on” fluorescent probe CPP based on self-assembled micelles for hydrolysis of lipase. The CPP probe exhibits high selectivity and excellent sensitivity for the detection of lipase in such a homogeneous system and is successfully applied for monitoring lipase activity in pancreatic AR42J cells, tissues, and serums. Taken together, the fluorescent CPP probe not only provides a tool for diagnostic potential in pancreatic disease but also demonstrates an application potential for micelle self-assembly-based development of biological probes. |
| first_indexed | 2024-12-24T03:11:52Z |
| format | Article |
| id | doaj.art-5755a2409c224c06bb8bb8b7464edfe2 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-12-24T03:11:52Z |
| publishDate | 2020-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-5755a2409c224c06bb8bb8b7464edfe22022-12-21T17:17:46ZengElsevieriScience2589-00422020-07-01237101294Detection of Lipase Activity in Cells by a Fluorescent Probe Based on Formation of Self-Assembled MicellesZhen Qiao0Hongyi Zhang1Yanru Zhang2KeWei Wang3Department of Medicinal Chemistry, Qingdao University School of Pharmacy, 308 Ningxia Road, Qingdao 266071, ChinaDepartment of Medicinal Chemistry, Qingdao University School of Pharmacy, 308 Ningxia Road, Qingdao 266071, ChinaDepartment of Medicinal Chemistry, Qingdao University School of Pharmacy, 308 Ningxia Road, Qingdao 266071, China; Institue of Innovative Drug Discovery, Qingdao University, 38 Dengzhou Road, Qingdao 266021, China; Corresponding authorDepartment of Pharmacology, Qingdao University School of Pharmacy, 308 Ningxia Road, Qingdao 266071, China; Institue of Innovative Drug Discovery, Qingdao University, 38 Dengzhou Road, Qingdao 266021, China; Corresponding authorSummary: Reliable and sensitive detection of lipase activity is essential for the early diagnosis and monitoring of acute pancreatitis or progression of digestive diseases. However, the available fluorescent probes for detection of lipase activity are only implemented in a hexane-water two-phase system due to the nature of heterogeneous catalysis of lipase, thus limiting their applications in direct imaging of lipase activity in living cells and tissues. Here we designed and synthesized a “turn on” fluorescent probe CPP based on self-assembled micelles for hydrolysis of lipase. The CPP probe exhibits high selectivity and excellent sensitivity for the detection of lipase in such a homogeneous system and is successfully applied for monitoring lipase activity in pancreatic AR42J cells, tissues, and serums. Taken together, the fluorescent CPP probe not only provides a tool for diagnostic potential in pancreatic disease but also demonstrates an application potential for micelle self-assembly-based development of biological probes.http://www.sciencedirect.com/science/article/pii/S2589004220304818Biophysical ChemistryBiocatalysisBiophysics |
| spellingShingle | Zhen Qiao Hongyi Zhang Yanru Zhang KeWei Wang Detection of Lipase Activity in Cells by a Fluorescent Probe Based on Formation of Self-Assembled Micelles iScience Biophysical Chemistry Biocatalysis Biophysics |
| title | Detection of Lipase Activity in Cells by a Fluorescent Probe Based on Formation of Self-Assembled Micelles |
| title_full | Detection of Lipase Activity in Cells by a Fluorescent Probe Based on Formation of Self-Assembled Micelles |
| title_fullStr | Detection of Lipase Activity in Cells by a Fluorescent Probe Based on Formation of Self-Assembled Micelles |
| title_full_unstemmed | Detection of Lipase Activity in Cells by a Fluorescent Probe Based on Formation of Self-Assembled Micelles |
| title_short | Detection of Lipase Activity in Cells by a Fluorescent Probe Based on Formation of Self-Assembled Micelles |
| title_sort | detection of lipase activity in cells by a fluorescent probe based on formation of self assembled micelles |
| topic | Biophysical Chemistry Biocatalysis Biophysics |
| url | http://www.sciencedirect.com/science/article/pii/S2589004220304818 |
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