Structural Characterization and Cytotoxic Activity Evaluation of Ulvan Polysaccharides Extracted from the Green Algae <i>Ulva papenfussii</i>
Ulvan, a sulfated heteropolysaccharide with structural and functional properties of interest for various uses, was extracted from the green seaweed <i>Ulva papenfussii</i>. <i>U. papenfussii</i> is an unexplored <i>Ulva</i> species found in the South China Sea alo...
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MDPI AG
2023-10-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/21/11/556 |
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| author | Vy Ha Nguyen Tran Maria Dalgaard Mikkelsen Hai Bang Truong Hieu Nhu Mai Vo Thinh Duc Pham Hang Thi Thuy Cao Thuan Thi Nguyen Anne S. Meyer Thuy Thu Thi Thanh Tran Thi Thanh Van |
| author_facet | Vy Ha Nguyen Tran Maria Dalgaard Mikkelsen Hai Bang Truong Hieu Nhu Mai Vo Thinh Duc Pham Hang Thi Thuy Cao Thuan Thi Nguyen Anne S. Meyer Thuy Thu Thi Thanh Tran Thi Thanh Van |
| author_sort | Vy Ha Nguyen Tran |
| collection | DOAJ |
| description | Ulvan, a sulfated heteropolysaccharide with structural and functional properties of interest for various uses, was extracted from the green seaweed <i>Ulva papenfussii</i>. <i>U. papenfussii</i> is an unexplored <i>Ulva</i> species found in the South China Sea along the central coast of Vietnam. Based on dry weight, the ulvan yield was ~15% (<i>w</i>/<i>w</i>) and the ulvan had a sulfate content of 13.4 wt%. The compositional constitution encompassed L-Rhamnose (Rha<i>p</i>), D-Xylose (Xyl<i>p</i>), D-Glucuronic acid (GlcA<i>p</i>), L-Iduronic acid (IdoA<i>p</i>), D-Galactose (Gal<i>p</i>), and D-Glucose (Glc<i>p</i>) with a molar ratio of 1:0.19:0.35:0.52:0.05:0.11, respectively. The structure of ulvan was determined using High-Performance Liquid Chromatography (HPLC), Fourier Transform Infrared Spectroscopy (FT-IR), and Nuclear Magnetic Resonance spectroscopy (NMR) methods. The results showed that the extracted ulvan comprised a mixture of two different structural forms, namely (“A3s”) with the repeating disaccharide [→4)-β-D-GlcA<i>p</i>-(1→4)-α-L-Rha<i>p</i> 3S-(1→]n, and (“B3s”) with the repeating disaccharide [→4)-α-L-IdoA<i>p</i>-(1→4)-α-L-Rha<i>p</i> 3S(1→]n. The relative abundance of A3s, and B3s was 1:1.5, respectively. The potential anticarcinogenic attributes of ulvan were evaluated against a trilogy of human cancer cell lineages. Concomitantly, Quantitative Structure–Activity Relationship (QSAR) modeling was also conducted to predict potential adverse reactions stemming from pharmacological interactions. The ulvan showed significant antitumor growth activity against hepatocellular carcinoma (IC<sub>50</sub> ≈ 90 µg/mL), human breast cancer cells (IC<sub>50</sub> ≈ 85 µg/mL), and cervical cancer cells (IC<sub>50</sub> ≈ 67 µg/mL). The QSAR models demonstrated acceptable predictive power, and seven toxicity indications confirmed the safety of ulvan, warranting its candidacy for further in vivo testing and applications as a biologically active pharmaceutical source for human disease treatment. |
| first_indexed | 2024-03-09T16:40:02Z |
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| language | English |
| last_indexed | 2024-03-09T16:40:02Z |
| publishDate | 2023-10-01 |
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| series | Marine Drugs |
| spelling | doaj.art-5756c2edc56c4d2d8af155563786aba92023-11-24T14:53:07ZengMDPI AGMarine Drugs1660-33972023-10-01211155610.3390/md21110556Structural Characterization and Cytotoxic Activity Evaluation of Ulvan Polysaccharides Extracted from the Green Algae <i>Ulva papenfussii</i>Vy Ha Nguyen Tran0Maria Dalgaard Mikkelsen1Hai Bang Truong2Hieu Nhu Mai Vo3Thinh Duc Pham4Hang Thi Thuy Cao5Thuan Thi Nguyen6Anne S. Meyer7Thuy Thu Thi Thanh8Tran Thi Thanh Van9NhaTrang Institute of Technology Research and Application, Vietnam Academy of Science and Technology, 02 Hung Vuong Street, Nhatrang 650000, VietnamSection for Protein Chemistry and Enzyme Technology, DTU Bioengineering-Department of Biotechnology and Biomedicine, Technical University of Denmark, 2800 Kongens Lyngby, DenmarkOptical Materials Research Group, Science and Technology Advanced Institute, Van Lang University, 69/68 Dang Thuy Tram Street, Ward 13, Binh Thanh District, Ho Chi Minh City 70000, VietnamNhaTrang Institute of Technology Research and Application, Vietnam Academy of Science and Technology, 02 Hung Vuong Street, Nhatrang 650000, VietnamNhaTrang Institute of Technology Research and Application, Vietnam Academy of Science and Technology, 02 Hung Vuong Street, Nhatrang 650000, VietnamNhaTrang Institute of Technology Research and Application, Vietnam Academy of Science and Technology, 02 Hung Vuong Street, Nhatrang 650000, VietnamNhaTrang Institute of Technology Research and Application, Vietnam Academy of Science and Technology, 02 Hung Vuong Street, Nhatrang 650000, VietnamSection for Protein Chemistry and Enzyme Technology, DTU Bioengineering-Department of Biotechnology and Biomedicine, Technical University of Denmark, 2800 Kongens Lyngby, DenmarkInstitute of Chemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Street, Hanoi 10000, VietnamNhaTrang Institute of Technology Research and Application, Vietnam Academy of Science and Technology, 02 Hung Vuong Street, Nhatrang 650000, VietnamUlvan, a sulfated heteropolysaccharide with structural and functional properties of interest for various uses, was extracted from the green seaweed <i>Ulva papenfussii</i>. <i>U. papenfussii</i> is an unexplored <i>Ulva</i> species found in the South China Sea along the central coast of Vietnam. Based on dry weight, the ulvan yield was ~15% (<i>w</i>/<i>w</i>) and the ulvan had a sulfate content of 13.4 wt%. The compositional constitution encompassed L-Rhamnose (Rha<i>p</i>), D-Xylose (Xyl<i>p</i>), D-Glucuronic acid (GlcA<i>p</i>), L-Iduronic acid (IdoA<i>p</i>), D-Galactose (Gal<i>p</i>), and D-Glucose (Glc<i>p</i>) with a molar ratio of 1:0.19:0.35:0.52:0.05:0.11, respectively. The structure of ulvan was determined using High-Performance Liquid Chromatography (HPLC), Fourier Transform Infrared Spectroscopy (FT-IR), and Nuclear Magnetic Resonance spectroscopy (NMR) methods. The results showed that the extracted ulvan comprised a mixture of two different structural forms, namely (“A3s”) with the repeating disaccharide [→4)-β-D-GlcA<i>p</i>-(1→4)-α-L-Rha<i>p</i> 3S-(1→]n, and (“B3s”) with the repeating disaccharide [→4)-α-L-IdoA<i>p</i>-(1→4)-α-L-Rha<i>p</i> 3S(1→]n. The relative abundance of A3s, and B3s was 1:1.5, respectively. The potential anticarcinogenic attributes of ulvan were evaluated against a trilogy of human cancer cell lineages. Concomitantly, Quantitative Structure–Activity Relationship (QSAR) modeling was also conducted to predict potential adverse reactions stemming from pharmacological interactions. The ulvan showed significant antitumor growth activity against hepatocellular carcinoma (IC<sub>50</sub> ≈ 90 µg/mL), human breast cancer cells (IC<sub>50</sub> ≈ 85 µg/mL), and cervical cancer cells (IC<sub>50</sub> ≈ 67 µg/mL). The QSAR models demonstrated acceptable predictive power, and seven toxicity indications confirmed the safety of ulvan, warranting its candidacy for further in vivo testing and applications as a biologically active pharmaceutical source for human disease treatment.https://www.mdpi.com/1660-3397/21/11/556ulvan<i>Ulva papenfussii</i>structural determinationanti-tumorigenicQSAR modeling |
| spellingShingle | Vy Ha Nguyen Tran Maria Dalgaard Mikkelsen Hai Bang Truong Hieu Nhu Mai Vo Thinh Duc Pham Hang Thi Thuy Cao Thuan Thi Nguyen Anne S. Meyer Thuy Thu Thi Thanh Tran Thi Thanh Van Structural Characterization and Cytotoxic Activity Evaluation of Ulvan Polysaccharides Extracted from the Green Algae <i>Ulva papenfussii</i> Marine Drugs ulvan <i>Ulva papenfussii</i> structural determination anti-tumorigenic QSAR modeling |
| title | Structural Characterization and Cytotoxic Activity Evaluation of Ulvan Polysaccharides Extracted from the Green Algae <i>Ulva papenfussii</i> |
| title_full | Structural Characterization and Cytotoxic Activity Evaluation of Ulvan Polysaccharides Extracted from the Green Algae <i>Ulva papenfussii</i> |
| title_fullStr | Structural Characterization and Cytotoxic Activity Evaluation of Ulvan Polysaccharides Extracted from the Green Algae <i>Ulva papenfussii</i> |
| title_full_unstemmed | Structural Characterization and Cytotoxic Activity Evaluation of Ulvan Polysaccharides Extracted from the Green Algae <i>Ulva papenfussii</i> |
| title_short | Structural Characterization and Cytotoxic Activity Evaluation of Ulvan Polysaccharides Extracted from the Green Algae <i>Ulva papenfussii</i> |
| title_sort | structural characterization and cytotoxic activity evaluation of ulvan polysaccharides extracted from the green algae i ulva papenfussii i |
| topic | ulvan <i>Ulva papenfussii</i> structural determination anti-tumorigenic QSAR modeling |
| url | https://www.mdpi.com/1660-3397/21/11/556 |
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