Antimicrobial resistance profiles and virulence genotyping of Salmonella enterica serovars recovered from broiler chickens and chicken carcasses in Egypt
Abstract Background This study aimed to survey the prevalence, antimicrobial resistance, and virulence-associated genes of Salmonella enterica recovered from broiler chickens and retail shops at El-Sharkia Province in Egypt. Salmonella virulence factors were determined using the polymerase chain rea...
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| Format: | Article |
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BMC
2019-04-01
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| Series: | BMC Veterinary Research |
| Online Access: | http://link.springer.com/article/10.1186/s12917-019-1867-z |
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| author | Rasha Elkenany Mona Mohieldin Elsayed Amira I. Zakaria Shimaa Abd- El-Salam El-sayed Mohamed Abdo Rizk |
| author_facet | Rasha Elkenany Mona Mohieldin Elsayed Amira I. Zakaria Shimaa Abd- El-Salam El-sayed Mohamed Abdo Rizk |
| author_sort | Rasha Elkenany |
| collection | DOAJ |
| description | Abstract Background This study aimed to survey the prevalence, antimicrobial resistance, and virulence-associated genes of Salmonella enterica recovered from broiler chickens and retail shops at El-Sharkia Province in Egypt. Salmonella virulence factors were determined using the polymerase chain reaction assays targeting the invA, csgD, hilC, bcfC, stn, avrA, mgtC, ompF, sopE1 and pefA genes. Results One hundred tweenty out of 420- samples from broiler chickens’ cloacal swabs, farm environmental samples, and freshly dressed whole chicken carcasses were positive Salmonella species. The isolates were serotyped as S. Enteritidis as the most dominant serotypes. Interestingly, none of the isolates were resistant to imipenem. The multidrug resistance was determined in 76.7% of the isolates with multidrug antibiotic resistance index of 0.2–0.6. Eight virulence genes (invA, csgD, hilC, stn, bcfC, mgtC, avrA, and ompf) were characterized among 120 S. enterica isolates with variable frequencies, while sopE1and pefA genes that were completely absent in all isolates. Based on the combination of presence and absence of virulence genes, the most common genetic profile (P7, 30%) was invA and csgD genes. Conclusion S. Enteritidis and S. Typhimurium were the most common identified serotypes in the examined sources. Circulation of such strains in broiler farms required introducing special biosecurity and biocontrol measures for control of Salmonella. Such measures might limit the adverse effects of antibiotics and ensure the safety of the environment and animal-derived food. |
| first_indexed | 2024-12-13T15:44:02Z |
| format | Article |
| id | doaj.art-5757d9c5dcb74981b437bb873f006eb0 |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-12-13T15:44:02Z |
| publishDate | 2019-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-5757d9c5dcb74981b437bb873f006eb02022-12-21T23:39:45ZengBMCBMC Veterinary Research1746-61482019-04-011511910.1186/s12917-019-1867-zAntimicrobial resistance profiles and virulence genotyping of Salmonella enterica serovars recovered from broiler chickens and chicken carcasses in EgyptRasha Elkenany0Mona Mohieldin Elsayed1Amira I. Zakaria2Shimaa Abd- El-Salam El-sayed3Mohamed Abdo Rizk4Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Mansoura UniversityDepartment of Hygiene and Zoonosis, Faculty of Veterinary Medicine, Mansoura UniversityDepartment of Food Hygiene and Control, Faculty of Veterinary Medicine, Mansoura UniversityDepartment of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, Mansoura UniversityDepartment of Internal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Mansoura UniversityAbstract Background This study aimed to survey the prevalence, antimicrobial resistance, and virulence-associated genes of Salmonella enterica recovered from broiler chickens and retail shops at El-Sharkia Province in Egypt. Salmonella virulence factors were determined using the polymerase chain reaction assays targeting the invA, csgD, hilC, bcfC, stn, avrA, mgtC, ompF, sopE1 and pefA genes. Results One hundred tweenty out of 420- samples from broiler chickens’ cloacal swabs, farm environmental samples, and freshly dressed whole chicken carcasses were positive Salmonella species. The isolates were serotyped as S. Enteritidis as the most dominant serotypes. Interestingly, none of the isolates were resistant to imipenem. The multidrug resistance was determined in 76.7% of the isolates with multidrug antibiotic resistance index of 0.2–0.6. Eight virulence genes (invA, csgD, hilC, stn, bcfC, mgtC, avrA, and ompf) were characterized among 120 S. enterica isolates with variable frequencies, while sopE1and pefA genes that were completely absent in all isolates. Based on the combination of presence and absence of virulence genes, the most common genetic profile (P7, 30%) was invA and csgD genes. Conclusion S. Enteritidis and S. Typhimurium were the most common identified serotypes in the examined sources. Circulation of such strains in broiler farms required introducing special biosecurity and biocontrol measures for control of Salmonella. Such measures might limit the adverse effects of antibiotics and ensure the safety of the environment and animal-derived food.http://link.springer.com/article/10.1186/s12917-019-1867-z |
| spellingShingle | Rasha Elkenany Mona Mohieldin Elsayed Amira I. Zakaria Shimaa Abd- El-Salam El-sayed Mohamed Abdo Rizk Antimicrobial resistance profiles and virulence genotyping of Salmonella enterica serovars recovered from broiler chickens and chicken carcasses in Egypt BMC Veterinary Research |
| title | Antimicrobial resistance profiles and virulence genotyping of Salmonella enterica serovars recovered from broiler chickens and chicken carcasses in Egypt |
| title_full | Antimicrobial resistance profiles and virulence genotyping of Salmonella enterica serovars recovered from broiler chickens and chicken carcasses in Egypt |
| title_fullStr | Antimicrobial resistance profiles and virulence genotyping of Salmonella enterica serovars recovered from broiler chickens and chicken carcasses in Egypt |
| title_full_unstemmed | Antimicrobial resistance profiles and virulence genotyping of Salmonella enterica serovars recovered from broiler chickens and chicken carcasses in Egypt |
| title_short | Antimicrobial resistance profiles and virulence genotyping of Salmonella enterica serovars recovered from broiler chickens and chicken carcasses in Egypt |
| title_sort | antimicrobial resistance profiles and virulence genotyping of salmonella enterica serovars recovered from broiler chickens and chicken carcasses in egypt |
| url | http://link.springer.com/article/10.1186/s12917-019-1867-z |
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